成纤维细胞的

Nerve repair at the level of spinal cord appeared as a new but challenging technique. The better understanding of regeneration from neurons and further refinement of instruments may push the brachial plexus surgery to a new level. Sensory and motor differentiated nerve coaptation plays an important role in the free neurovascular muscle transplantation due to the significance of motor axons for the flap reinnervation.

脊髓水平的神经修复是一个新而具有挑战性的技术，对神经元再生的深入理解（包括神经元误向生长与存活问题）与实验手段的完善（包括基因调控的成纤维细胞和雪旺细胞种植等），将使臂丛外科进入一个新的水平。

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

A virus strain was isolated from the arthrosis of broiler chickens.The virus was shown to have spherical particles with isometric symmetry,no envelope,double deck capsids,with outer capsid of 75nm,inner one of 50nm in diameter by electron microscopy.The virus was not sensitive to aether,acid and heat.The virus could multiply on the chick embryo fibroblastcell,and could cause syncytial pathologic change with the appearance of cytoplasm inclusion bodies.The challenged chickens of one-day old by nasal,oral,intramuscular and pulvillus injection displayed obvious clinical symptom of severe arthritis and accompanied with pathological changes of malabsorption and necrosis.Meanwhile,four avian reovirus isolates CL,S1133,YH and YB were examined in the cross-neutralization test.

从临床患病鸡关节病料中分离到一株病毒，在电镜下观察到病毒粒子无囊膜，有双层衣壳，外衣壳直径约75nm，内核约50nm；分离病毒株对酸、热有较强的抵抗力，对乙醚不敏感；病毒感染鸡胚成纤维细胞上能产生以融合细胞为特征的CPE；爪垫接种1日龄雏鸡表现出明显的病毒性关节炎临床症状，并伴有吸收障碍型和坏死型的病理变化；血清中和交叉试验表明，该病毒分离株与国内的2株番鸭源呼肠孤病毒分离株和国外S1133呈不同程度的交叉反应。

Primordial germ cells derived from gonadal ridges and mesenteries of 30-70-day post menopause human ernbiyos, when cultured on mouse MEF or STO fibroblast feeder layers in presence of hrLW , hrbFGF, hrSCF, and forskolin could survive and give rise to multicellular colonies, resembLing mouse ES-like colonies .

从停经30-70天流产的早期胚胎后肠系膜中或生殖嵴部位分离到的原始生殖细胞，在小鼠的原代MEF或STO成纤维细胞等饲养层细胞上培养，能在hrLIF、hrFGF、hrSCF、forskolin等的共同作用下存活并增殖。

METHODS: BMSCs were obtained from Japanese big-eared rabbits, and in vitro cultured. Then the subcultured BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry.

日本大耳兔骨髓间充质干细胞进行体外培养扩增后，通过pCDNA3.1质粒将碱性成纤维细胞生长因子基因转染至生长状态良好的骨髓间充质干细胞，并将转染后的细胞接种于制备好的猪小肠黏膜下层上，进行体外联合培养，构建组织工程皮肤。

Fibroblast growth factor is a kind of the polypeptide growth factors, which is known to be a mitogen for a wide variety of mesoderm-and neuroectoderm-derived cells and a neurotrophic factor for several neuronal cell types.

成纤维细胞生长因子是一种对中胚层及神经外胚层源的细胞都有促分裂活性的多肽生长因子，也是一种重要的神经营养因子。

11 guinea pigs which were successfully induced myopias were extracted eyeballs, and the scleras near posterior poles were observed by immunohistochemistry methods. The positive rates of TGF-β〓 and bFGF in scleral fibroblast cytoplasma and the area percentageratio of positive cells were calculated and contrasted to controlled eyes.

将透镜诱导近视成功的11只豚鼠处死，摘除眼球，取后极部巩膜分别进行TGF-β〓和bFGF的免疫组织化学染色，观察它们在巩膜组织成纤维细胞胞浆中中表达的阳性率和阳性细胞所占面积的百分比，并与对照眼进行比较。

11 guinea pigs which were successfully induced myopias were extracted eyeballs, and the scleras near posterior poles were observed by immunohistochemistry methods. The positive rates of MMP-2 and TIMP-2 in scleral fibroblast cytoplasma and the area percentageratio of positive cells were calculated and contrasted to controlled eyes.

将透镜诱导近视成功的11只豚鼠处死，摘除眼球，取后极部巩膜分别进行MMP-2和TIMP-2的免疫组织化学染色，观察它们在巩膜组织成纤维细胞胞浆中表达的阳性率和阳性细胞所占面积的百分比，并与对照眼进行比较。

Screened with cOnidia of hauIthe mp, the bo of myce1iageednated frOm cOnidia of Prtubo mp was Observd Whe the mixtUr of CS-l l andcs-l2, aS well aS the COmPoUnd cs-7 were Presot. And the mtw of Cs-1l and cs-12shOwed grOWth-inhibitory activity ofendothlial cells and NIH-3T3 fibrobast in the.

稻瘟霉活性筛选结果显示CS-11和CS-12的混合物、CS-7具有使稻瘟霉孢子菌丝体变形的作用；体外药理实验显示，CS-11和CS-12的混合物具有显著的体外抑制血管内皮细胞生长作用及抑制血管成纤维细胞生长作用。

Immunohistochemical staining revealed that the reactivities of mAbs to the lymphocyte cells in tonsil and colon tissues, to fibroblast cells in foreskin tissues, to macrophages in lung tissues were observed.

免疫组化染色表明，mAb与人扁桃体、结肠组织中的淋巴细胞、包皮组织中的成纤维细胞、肺组织中的巨噬细胞反应呈阳性。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。