成纤维细胞的

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

Objective: To observe the attachment and growth of fibroblasts on a new porcine collagenic dermal matrix and investigate a method to improve the cell compatibility of the porcine dermal scaffold.

目的：观察成纤维细胞在一种新型的猪胶原基真皮支架上的粘附生长特性，探讨提高异种真皮支架细胞相容性的方法。

2The distribution of HIF-1 a and COX-2 was unequal;.(3)These findings indicate that HIF-1a and COX-2 may be two important factors in pulpal lesions and there is a significantly hypoxia condiction in inflamed pulp .(4)The increase of HIF-1 a is a possible mechanism for hypoxia preconditioning .(5)Our results suggested that pulpal fibroblasts as well as macrophages might be involved in the pathogenesis of irreversible pulpitis.

和COX一2的表达均有不同程度的变化，在牙髓炎病程中可能发挥着重要的生理和病理作用，③在牙髓炎进程中和牙髓自身修复过程中存在着缺氧环境和缺氧耐受机制，④牙髓成纤维细胞有可能与单核巨噬细胞一同参与牙髓炎的病理进程，且具有修复和免疫功能。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象，采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法，研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

Fibroblast growth factor 21（FGF21）is a novel fatty metabolic regulator. FGF21 mRNA is most abundantly expressed in liver. FGF21 contribute to fat metabolism, as well as ketogenic responses in liver,and it can promote the uptake of glucose, promote insulin secretion, delaying the development of tumors and other function.

成纤维细胞生长因子21（fibroblast growth factor 21，FGF21）是一种主要的脂肪代谢调节因子，主要在肝脏中表达；FGF21有助于肝脏的脂肪代谢以及生酮反应，可以促进脂肪细胞摄取葡萄糖，促进胰岛素分泌，延缓肿瘤的发展等功能。

Objective The expression of iNOS bFGF CyclinD1 erbB-2 were studied by immunohistochemistry to investigate the relationships between the expression of iNOS and proliferative index of breast carcinoma such as bFGF CyclinD1 and erbB-2.The relationship between expression of iNOS and ages menstruous status tumor size histological stage TNM stage venous invasion ER PR lymph-node metastasis distant metastasis and survival rate in 5 years.

目的 研究诱导型一氧化氮合酶(Inducible Nitric Oxide，iNOS)在乳腺癌中的表达及与乳腺癌增殖指标碱性成纤维细胞生长因子、细胞周期素D_1(CyclinD_1)、癌蛋白(erbB-2)的关系，同时研究iNOS与年龄、月经状况、肿瘤大小、肿瘤学分级、TNM分期、脉管侵袭、ER、PR、淋巴结转移、远处转移、预后的关系。

tissue engineering ; osseocartilaginous ; adipose-derived stem cells

近年来，学者们在多个物种的脂肪组织中发现具有多向分化潜能的成纤维细胞样细胞，在体外特定的诱导条件下可以分化为

AIM: To observe the effect of diterpenoid compound 5F of Pteris semipinnate L on apoptosis in cultured human pterygium body fibroblasts. METHODS: Fibroblasts collected from human pterygium body were cultured in vitro with 5F at different time point.

目的：观察中药半边旗二萜类化合提取物5F对体外培养的人翼状胬肉成纤维细胞周期的影响及5F诱导细胞凋亡过程超微结构的变化，初步探讨5F对HPFs作用的机制。

Copper levels in skin fibroblasts of the involved vitiliginous skin were much lower than those of the uninvolved, but slighty lower than or the same with those of controls.

近年来发现白瘫风患者的血清铜和铜蓝蛋白含量降低囚，作者对白瘫风患者的皮损区、周围区的皮肤成纤维细胞进行了细胞培养和铜含量测定。

ZHAO Yede*,HE Qinglian.Department of Plastic Surgery, Navy 411 Hospital, Shanghai 200081Objective To investigate the expression level and intracellular distribution of calreticulin in hypertrophic scar-derived fibroblastsand normal skin-derived fibroblasts.

增生性瘢痕(Hypertrophic scar， HS)作创伤及外科手术后常见且十分棘手的皮肤组织疾病，瘢痕组织内成纤维细胞异常活化和细胞外间质过度沉积既是HS的重要组织学特征，也是HS发生、发展的物质基础。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。