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The goat fetal fibroblast cells were transfected by linear GBCt-PAm with electroporation or lipidosome, and after the identification of PCR, we got 7 lines of progenitive cells that examined positive.The two cell line (M9, M10) had been used for the somatic nuclear transfer for the first time.

将pGBCt-PAm去除原核序列后回收线性片段,采用电击和脂质体转染两种方式转染山羊胎儿成纤维细胞,经G418筛选培养基筛选及PCR检测,最终得到了7株能有效扩增的PCR检测阳性的细胞克隆。

Mast cells w ere isolated and purif ied from human embryo oral mucosa and were co-cultured with the fibrobl asts from human embryo oral mucosa.The rough endoplasmic reticula a nd mitchondria of OMFb were observed and analyzed.

采用分离纯化人胚口腔粘膜肥大细胞的介质溶液与体外培养的人胚口腔粘膜成纤维细胞共同培养,对OMFb进行形态计量分析。

However, it is still difficult to find out the real reason of the increase of the keratinized gingiva during the orthodontic extrusion whether it is due to the proliferation of the gingival tissue or to its elastic nature, that is because the proliferation of the fibroblasts as well as the connective tissue changes in volume and the intra-fibers spaces were all immeasurable in most of the studies.

然而,仍然难以找到真正的原因增加的角化牙龈在正畸挤压它是否是由于扩散的牙龈组织或其弹性性质,这是因为增殖的成纤维细胞以及作为结缔组织的变化量和内部纤维空格都是不可估量的,大多数的研究。

The hEFs derived from gonadal ridges and dorsal mesenteries of human embryos are successfully isolated and cultivated,and the cells express the cytokines essential for the growth of hEG in vitro.

成功地分离和培养了人胚胎生殖腺嵴和肠背系膜来源的成纤维细胞,证明其表达对于hEG体外生长起重要作用的细胞因子。

The endothelial cells cocultured with fibroblasts from scar had greater proliferation rate than that from normal skin.

另外,Transwell 共培养结果发现,增生性瘢痕来源的成纤维细胞具有显著促进血管内皮细胞增殖的作用。

We used the NIH3T3 fibroblast cells ascarrier because the cell clone is easy to expand,to betransfected and to express the exogenous gene.In the presentstudy,a model of fibro blast cell-mediated human IFN-〓 genetherapy was established and its anti-tumor activity was observed.

为此,我们选择了易于扩增、易于转染并能稳定表达外源目的基因的成纤维细胞为载体细胞,建立了人〓基因疗法的实验模型,并观察了其体内抗肿瘤作用。

Fluid nitrogen cryopreservation has no obvious effect on the MHC antigeniacity of aortic homografts of rats.

液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞MHC类的抗原性没有明显的影响。

SEM showed that after the absence of HA granules from the surface, fibroblast started ingrowing and fresh callus came into being. This indicated that HA/PDLIA composite material had certain bioactivity and bone connectivity.

通过SEM研究表明:HA颗粒从材料表面脱落后,成纤维细胞向组织内长入,并伴有少量新生骨痂的形成,显示HA/PDLLA复合材料具有一定的成骨性和骨连接性。

BACKGROUND: Basic fibroblast growth factor can accelerate the bone marrow mesenchymal stem cells proliferation and differentiation into nerve cells, which is considered as a mitogen of glial cells.

背景:碱性成纤维细胞生长因子可以促进骨髓间充质干细胞的增殖和向神经细胞方向分化,并被认为是胶质细胞的分裂原。

METHODS: Keloid tissues with prominences and redness were harvested. Subsequent to primary cultures, dermal fibroblasts at passages 3 to 4 were used in this study.

取瘢痕疙瘩边缘突起发红的部分,按组织块法培养原代成纤维细胞,取第三四代的细胞用于实验。

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