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Objective To determine the content change of vascular endothelial growth factor and basic fibroblast growth factor in the wound fluid and their effect on angiogenesis in the wound healing during healing of maxillofacial soft tissue blast injury. Methods The improved rabbit model of maxillofacial blast injury was made by KTY-04 blasting cap.

摘 要:目的探讨颌面部软组织爆炸伤愈合过程中血管内皮细胞生长因子和碱性成纤维细胞生长因子在伤口液中含量的动态变化及其对创伤愈合中血管再生过程的刺激作用。

Embryonic germ cell lines were isolated and cloned from gonad,gonadal ridge,analogue or the surrounding tissues of 9.5~13.5 days postcoitum mouse fetuses.Primordial germ cells were co-cultured with mouse embryonic fibroblasts obtained from the same conceptus.

自交配后9.5~13.5 d小鼠胎儿生殖嵴/腺或其类似物及周围组织,采用与其同源胎儿成纤维细胞共培养的方式分离克隆到具小鼠ES细胞诸多特征的胚胎生殖细胞系。

AIM: To study the effects of basic fibrolast growth factor on the proliferation of lymphokine activated killer cells from patients with bladder cancer and LAK cells cytolysis against bladder tumor cells.

目的:探讨白细胞介素Ⅱ(IL2)和碱性成纤维细胞生长因子对膀胱癌患者淋巴因子激活的杀伤细胞的作用。

After 24 h, a little culture medium supplied until fragmental tissue were just submerged.

对成纤维细胞采取了消化的方法,经2~3次传代后获得较为纯净的肿瘤细胞,现在已经连续传代12次以上,建立了有限细胞系,命名为SPA-02。

Kase S, Sugio K, Yamazaki K, et al. Expression of Ecadherin and betacatenin in human nonsmall cell lung cancer and the clinical significance[J].

CD44是分布极为广泛的细胞表面跨膜糖蛋白,在淋巴细胞,成纤维细胞表面均能检测到它的表达。

Objective: To investigate the nephrotoxicity of plumbagin on human embryonic kidney cell 293 in vitro and protective effects of acid fibroblast growth factor on the cells.

目的:观察白花丹素在体外对人胚肾细胞293的毒性,以及酸性成纤维细胞生长因子对白花丹素所致肾损伤的保护作用。

Recent work has shown that mouse and human fibroblasts can be to a pluripotent state with a combination of four transcription factors.

最近的工作显示人和鼠的成纤维细胞可以通过转入4个因子而重新编程为多功能细胞。

ConclusionPressure force modulates the cell cycle time and the PI of fibroblast cells in fibrous tissues restrainedly, which may be one of the possible cytobiological principles of that dredging the channels and eliminating stagnation submitted by massage therapy.

结论压力刺激对筋膜组织成纤维细胞周期与PI的抑制性调节,可能是推拿"舒筋散结"的细胞生物力学原理之一。

Objective To observe the effects of IL-1α derived from keratinocytes on proliferation and collagen secretion of fibroblasts.

目的:研究角质形成细胞分泌的IL-1α对成纤维细胞生物学行为的影响。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4/brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸,干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4/brn4的表达,免疫印迹检测反义干扰细胞POU3F4/brn4蛋白条带的强弱,RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化,观察POU3F4/brn4对COCH基因的转录调控作用。

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