成纤维细胞的

This study investigated the mechanisms that induce EMT and the significance of extracellular signal-regulated kinase cascade in this course in vitro.

本文旨在研究酸性成纤维细胞因子对上皮-间质转分化的影响，探讨有丝分裂原激动蛋白激酶的ERK信号传导途径在此过程中的作用，为肾小管-间质纤维化的防治提供理论依据。

Objective:To study the effect of spironolactone inhibiting proliferation on cultured myocardiac myofibroblasts and explore its possible mechanisms.

目的：研究螺内酯对培养的心肌成纤维细胞增殖的作用，揭示螺内酯抑制心肌纤维化的可能机制。

On base of this, we investigated the effect of the physical property of scaffolds and fibroblast coculture on hepatic differentiation of hADSCs.

在此基础上进一步探讨PLGA支架物理特性和成纤维细胞共培养对人脂肪干细胞向肝细胞分化的影响。

RESULTS: Stable telomerase activity could be detected in the transfected fibroblasts and no signs of cell senescence were found in the fibroblasts cultured for more than 50 doublings.

结果 转染 h TERT的人成纤维细胞能稳定表达端粒酶活性，培养超过 5 0代后细胞仍保持β-半乳糖苷酶阴性。

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞，原代培养2d后改变培养基，添加含20%胎牛血清的DMEM（含25ml/L葡萄糖，100U/ml青链霉素，pH值7.6），添加烟酰胺10mmol/L，胰岛素1mg/L，N2添加剂，碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

The multiplication conditions of bone marrow stromal cells were respectively detected by fibroblast colony forming in vitro, tritiated thymidine {[3H]TdR} incorporation and MTT.

分别应用成纤维细胞体外集落形成法、[3H]TdR掺入法和四甲基偶氮唑盐法检测骨髓基质细胞的增殖情况。

Methods The guts of embryonic mice removed and dissociated were plated into serum-free DMEM/F12 medium. The mitogen-free DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Methods The guts of embryonic mice removed and dissociated were plated into serumfree DMEM/F12 medium. The mitogenfree DMEM/F12 medium supplementd with 10% fetal bovine serum was used to induce differentiation of GNCSCs. Neurospheres and their derivations were determined with immunocytochemical and immunofluorescent staning.

分离胚鼠肠管，消化后接种于添加N2、B27和碱性成纤维细胞生长因子的DMEM/F12培养基，贴壁培养；连续传代后用血清促进GNCSCs分化；最后用免疫细胞化学方法染色鉴定。

Results:bFGF is a biological factor,which can induce cellular proliferation of bone tissue and vascular tissue.

结果：碱性成纤维细胞生长因子是一种生物因子，其可以诱导骨组织及血管组织细胞的分裂。

Fibroblasts have been extensively used for a wide range of cellular and molecular studies.

成纤维细胞被广泛地用于细胞和分子领域的研究。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。