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The surface antigen expressions of MNC were detected by flow cytometry. MNC were induced with DMEM medium, containing HGF alone, FGF4 alone, HGF+FGF4 or no growth factor, respectively. The markers of hepatic linear cells were examined before induction, and 7, 14, 21 and 28 d after induction by RT-PCR, immunocytochemistry and periodic acid-schiff in every group.

分别用肝细胞生长因子、成纤维细胞生长因子-4(FGF4)、HGF+FGF4、无生长因子4种处理因素进行诱导培养,于诱导前及诱导后的第7、14、21、28天采用RT-PCR检测AFP、白蛋白及C-met、FGFR2 mRNA的表达,免疫细胞化学法检测肝细胞抗原和CK18的表达,PAS 法进行糖原染色。

To study the effect of basic fibroblast growth factor and nerve growth factor on immune function in mice. Methods: Through the assays of Phagocyte, PFC, MTT and ELISA, the action of Mφ, B lymphocyte and T lymphocyte of mice was observed.

细胞生长因子是多功能的生物活性多肽,包括成纤维细胞生长因子、神经生长因子和表皮生长因子等,作为在细胞间传递信息的物质,细胞生长因子在机体内发挥着极其广泛的生物学作用,参与多种病理生理过程,如生长、免疫、肿瘤、创伤愈合等。

RESULTS: Persistent positive expression of vascular endothelial growth factor was seen in the epidermal cells and sebaceous gland cells.

阳性细胞数量随损伤时间的延长而增多,于伤后96 h达峰值,伤后1周和2周仍见少量巨噬细胞和成纤维细胞表达阳性。

To explore the potential mechanism of cigarette smoke extract -induced cell injury, primary cultured skin keratinocytes and fibroblasts from rat were selected as target cells in Part Ⅰ study compared with the effect of UVA.

本研究第一部分以原代培养的大鼠皮肤角质形成细胞和成纤维细胞为对象,采用UVA损伤作用为参照,探索香烟雾提取物(Cigarette SmokeExtract,CSE)对皮肤细胞的损伤作用,重点观察CSE所致的皮肤细胞毒作用、功能损伤及可能作用机制。

Basic fibroblast growth factor is a potent mitogen for a variety of mesoderm- and neuroectoderm-derived cells and has been found in aqueous humor, vitreous and other ocular tissues.

碱性成纤维细胞生长因子(basic fibroblast growth factor bFGF)是对广泛来源于中胚层和神经外胚层细胞的有效促细胞分裂因子,已在房水、玻璃体和其它眼组织中发现。

One eye in patients was selected to do the tear film breakup time, Schirmer test and cornea fluorescein staining, and the expression of NFκB and TGFβ1 on bulbar conjunctiva endepidermis was detected by immunohistochemistry.

TGFβ1是多功能细胞因子,对上皮细胞具有较明显的抑制作用,对成纤维细胞及其他间叶组织来源的细胞均具有明显的刺激作用,表现为胶原合成增加,有利于组织修复及伤口愈合[3,4]。

BACKGROUND: Chondrocytes in vitro cultured with traditional culture methods tend to dedifferentiate into fibroblasts through long-term serial subcultivation, which results in a terrible decrease in the number and activity of cells.

背景:传统培养方法体外培养的软骨细胞经长时间传代培养后往往去分化为成纤维细胞,导致细胞数量及活性下降。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中,地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长;实验组创面愈合时间较对照组提前了4天;第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多,上皮细胞分裂象也高于对照组,第4天的间皮细胞分裂象也高于对照组;自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

These nonmalignant cells, such as stromal fibroblasts and bone marrow-derived cells, have been shown to be capable of promoting tumor growth and metastasis.

这些非恶性细胞如基质成纤维细胞和骨髓源性细胞已被证明能够促进肿瘤的生长和转移。

The therapeutic mechanism of the method of nourishing qi, activating blood, dispersing phlegm and promoting the collaterals was supposed as that it intervened the airway remodeling and alleviated the progress of airflow obstruction of COPD by suppressing the chemo taxis of cytokines, regulating the proliferation of fibroblast cell and smooth muscle cell and preventing the proliferation and plerosis of extracellular matrix.

推测益气活血化痰通络法防治COPD的机制可能为:通过抑制炎症细胞的趋化、影响细胞因子的活性、调节成纤维细胞和平滑肌细胞的增殖、阻止细胞外基质的增生修复,从而达到干预COPD患者气道结构重塑,减缓COPD气流受限进展的作用。

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