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It has several kinds of functions : It could accelerate blood coagulation; has chemotaxis to monocyte and neutrophil , could appeal to fibroblast and cartilage cell to move to the region of the injury, takes part in the signal transduction and activation in cell , and takes part in the growth and differentiation of cell and repair in trauma.

它的功能多样,能加速血液凝固,对单核细胞和中性粒细胞具有化学趋化作用,能吸引成纤维细胞和软骨细胞向损伤区域移动,参与细胞的信号传导与活化,细胞的生长及分化,创伤修复等一系列重要生理和病理过程。

Effect of negative electrets enhancing cell growth and increasing cell surface charge was in proportional to the surface potential of electret.

2成纤维细胞表面负电荷和S期内细胞数的增加量与驻极体的表面电位成正比。

Objective: To study the influence of electret on surface charge of fibroblast cells (3T3 cells) and to probe the relationship between cell growth, apoptosis and cell surface charge.

目的:研究驻极体对成纤维细胞表面电荷的影响及其细胞表面电荷与细胞生长、细胞凋亡的关系。

At 18 and 24 hours after culture, types of cell death were quantitatively detected using fluorescein isothiocyanate and propidium iodide staining.

25%,50%,100%浓度的3种材料浸提液体外培养小鼠成纤维细胞L929 24 h后,采用流式细胞术碘化丙啶染色法检测L929细胞的凋亡率及细胞周期分布情况。

The basic pathological change of silicosis is the lung interstitial fibrosis and the formation of silicotic nodule.

与矽肺的发病相似,特发性肺纤维化也是以肺成纤维细胞大量增殖为主要特征的肺纤维化疾病。

RESULTS: hADSCs presented fibroblast-like morphological feature with a flocked array. The 3rd passage of hADSCs had unique immunophenotypes and they were positive for CD73, CD44, CD166, CD105 and CD29, but negative for CD31, CD34, CD45 and HLA-DR. Cell cycle result showed that they had the typical growth characteristics of stem cells, namely, 83.81% cells stayed at G0/G1 stage, only 16.19% cells were stayed at S+G2/M stage; The latent phase of the primary culture cells was 2 days prior to and after incubation, followed by 3-6 days of logarithmical proliferation, reached a peak at day 6, and entering the growth platform phase with lower growth speed.

结果:分离培养的人脂肪间充质干细胞为成纤维细胞样,呈漩涡状排列。P3代人脂肪间充质干细胞呈CD73,CD105,CD166,CD44及CD29阳性,而CD31,CD34,CD45及HLA-DR阴性;具有典型的干细胞增殖特点,83.81%细胞处于G0/G1期,仅16.19%细胞处于S+G2/M期;细胞在接种后前2 d处于生长潜伏期,第3~6天处于对数生长期,第6天达峰值,以后细胞生长速度减慢,进入生长平台期,平均五六天传代1次。

METHODS By means of cell culture and flow cy-tometry we studied the effect of fluid nitrogen cryopreser-vation on the expression of MHCⅠantigen of smooth mus-cle cells and fibroblasts of aortic homografts of rats.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

METHODS By means of cell culture and flow cytometry we studied the effect of fluid nitrogen cryopreservation on the expres sion of MHC Ⅰ antigen of smooth muscle cells and fibroblasts of aortic homografts of ra ts.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用,按美国ASTM标准制备NiTi、317L合金的金属浸提液;&组织块培养法&原代培养食管壁疤痕的成纤维细胞,传代后以金属浸提液进行培养,分组后分别培养4、24、48、72 h,MTT法检测不同培养时间后Fb增殖功能的变化;按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验,即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间,术后2、12周取出包埋组织,分析试件周围组织的病理变化,并进行胶原纤维染色,观察纤维形成状况。

Basic fibroblast growth factor ; lymphokine activated killer cells ; cell division ; interleukin 2; bladder neoplasms ; immunologic cytotoxicity

碱性成纤维细胞生长因子;淋巴因子激活的杀伤细胞;细胞分裂;白细胞介素-2;膀胱癌;免疫细胞毒性

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