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At 12w after operation, the cells in control group still behaved as fibroblasts and fibrocartilage like cells were predominant in grafts of cell and gene groups , the grafts in these two groups were similar to normal ligament morphologically.

手术后12周,对照组显现移植物内有大量的成纤维细胞,甲苯胺兰染色轻度异染;而细胞组和基因组细胞量减少,显现类软骨性状,细胞排列与正常前交叉韧带接近。

And, normal keratinization can be observed by scanning electron microscape on the surface of the PCS.

通过冻干法制备的蛋白改性壳聚糖多孔支架对细胞没有毒性,L929细胞和人成纤维细胞可以在支架表面和内部正常生长和增殖,角质细胞在材料表面可以正常老化、角质化。

RESULTS: Otocyst cells differentiated and proliferated and showed several morphotypes, mainly including epitheliallike cells, fibroblastlike and neuronlike cells.

结果:培养的细胞在体外进行分化和增殖,细胞形态主要有上皮细胞形态、成纤维细胞形态和神经细胞形态3种。

RESULTS摘要: Otocyst cells differentiated and proliferated and showed several morphotypes, mainly including epitheliallike cells, fibroblastlike and neuronlike cells.

结果摘要:培养的细胞在体外进行分化和增殖,细胞形态主要有上皮细胞形态、成纤维细胞形态和神经细胞形态3种。

The first passage of YS-MSCs which grew in good condition was obtained, and adipogenic differentiation of YS-MSCs was induced by dexamethasone and insulin, and angioblast was induced by vascular endothelial growth factor and basic fibroblast growth factor.

取生长状态良好的第1代细胞,以地塞米松、胰岛素定向诱导卵黄囊间充质干细胞向脂肪细胞方向分化;以血管内皮生长因子和碱性成纤维细胞生长因子体外诱导其向血管内皮细胞方向分化。

Objective To explore the effect of myogenic transcription factor myogenic determination gene on the differentiation of fibroblasts into myoblasts in rats in vitro.

目的 探讨MyoD在体外诱导大鼠成纤维细胞分化为成肌细胞中的作用。

MethodsThe transplanted tumor model of the mouse S180 sarcoma was established.Fifty mice were randomly divided into five groups,the control group,the CTX group,the Gecko group.They were treated respectively with oral administration of saline,and intraperitoneal injection of CTX 100 mg/kg only one time,oral administration of Gecko in doses of 13.5,9,4.5 g/kg,one time a day.After 14 days,the anti-tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected by accounting thymus index, spleen index and the number of peritoneal macrophage which phagocytose chicken red blood cells.The protein express of vascular endothelin growth factor and basic fibroblast growth factor were detected by SABC immunohistochemistry.

方法建立移植瘤小鼠S180肉瘤模型,将50只雌鼠随机分为对照组、环磷酰胺组、壁虎高组、中组、低组共5组,分别给于生理盐水灌胃1次/d,CTX(100 mg/kg)腹腔注射1次,壁虎高、中、低组(13.5,9,4.5 g/kg),每天灌胃1次。14 d后,称取荷瘤小鼠瘤重、胸腺重、脾脏重,计算抑瘤率、胸腺指数、脾脏指数,观察腹腔巨噬细胞吞噬鸡血红细胞实验指标;用SABC免疫组化法检测血管内皮生长因子、碱性成纤维细胞生长因子的蛋白表达,TUNEL方法检测细胞凋亡率。

The reverse transcription-polymerase chain reaction was used to seek the expressions of a specific fibroblast marker, prolyl 4-hydroxylase β, and a specific marker of epithelial cells, cytokeratin-4, in the cells.

采用逆转录多聚酶链反应检测hEFs表达成纤维细胞特异性标志(脯氨酰4-羟化酶β亚单位)和上皮细胞特异性标志(细胞角蛋白4)的情况,对该细胞进行鉴定。

In both inflammatory and healthy gingival epithelium, CD14 mRNA positive cells, which are mainly located at thornlike cell layer, were found.

另一方面,牙髓组织除树突状细胞外CD14表达细胞很少,牙龈组织皮下的成纤维细胞和未分化细胞也不表达CD14。

He expression of TLR4 is also increased in the peripheral blood monocytes from the patients with ACS.

LR4在动脉粥样斑块的内皮细胞、巨噬细胞、血管平滑肌细胞和血管外膜成纤维细胞表达均增高,在急性冠脉综合征患者外周血单核细胞表达增高。

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