成纤维细胞的

The histopathologic observations of corneas after penetrating keratoplasty in dogs: Thestructure of wholly transparent graft was the same as that of host cornea apart from the upperdonor-host junction where there was minimal irregularity of the corneal lamellae. However, thecorneal lamellae at the lower donor-host junction were neat. The graft with its central part beingtransparent only showed poor coaptation with recipient, in which the graft shifted inward and had marked wrinkles and folds in Descemet"s membrane. On the back of Descemet"s membranethere was hyperplastic stromal fibers that thickened the graft and partially adhered to iris.

5犬穿透性角膜移植术后角膜病理组织学观察完全透明的植片与植床结构一致，仅两者对合处上部纤维紊乱，而下方纤维排列整齐；中央透明的植片与植床对合不良，植片向植床方向嵌入，后弹力层皱缩、折叠，植片下方因有来自植床的基质纤维而增厚，部分对合处与虹膜粘连；浑浊的植片有比较完整的上皮层，植片约2／3深度的基质丧失原来纤维整齐排列结构，代以大量成纤维细胞增生。

It's also where cells called fibroblasts produce collagen and elastin fibers.

它的细胞，也是所谓的成纤维细胞产生胶原蛋白和弹性蛋白纤维。

Results:Wound healing rate in group A was higher than that in group B and the wound healing time was 1～3 days earlier in group A.More active proliferation of epidermic cells around wounds showed in group A.The function of fibroblasts in wounds,collagen synthesis and secretion,were more vigorous in group A than those in group B.

结果：A组创面愈合率高于B组，其创面愈合时间较B组提前1～3 d；创周表皮细胞增殖也较B组活跃，创面成纤维细胞较B组呈现出更旺盛的合成与分泌胶原纤维的功能。

Many factors may be involve in the course. To investigate the regulation activity of mesenchymal cells to differentiation of epithelial cells from hair follicle and to study its differentiation property, mesenchymal cells gel was made by nubby dermal papilla cells, free dermal papilla cells, skin fibroblasts. Skin keratinocytes and epithelial cells from hair follicle were inoculated on the gel surface and cultured in air-liquid interface. Three-dimensional model of DPC using to induce epithelial cells differentiation is built in vitro.

为了进一步研究毛囊细胞间的相互作用，探讨毛囊间质细胞对毛囊上皮细胞分化的调节作用，研究毛囊上皮细胞的分化特性，我们利用团块状的毛乳头细胞，游离分散的毛乳头细胞或皮肤成纤维细胞制成间质细胞胶原凝胶，表面接种皮肤角质形成细胞或毛囊上皮细胞，进行气-液界面培养，在体外建立了毛乳头细胞诱导毛囊上皮细胞分化的立体模型。

Skin keratinocytes and epithelial cells from hair follicle organized into epidermoid cyst-like spheroids when cultured on nubby dermal papilla cells gel and epidermoid layer-like structure was formed when they were cultured on free dermal papilla cells and skin fibroblasts gel.

团块状的毛乳头细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成球形结构；而游离分散的毛乳头细胞和皮肤成纤维细胞诱导毛囊上皮细胞和皮肤角质形成细胞形成表皮样层化结构。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

The conclusions were that the inhibitory effect of SM was the result of inhibiting the mitosis of cells and the cellular cycle be at a standstill in G2-M stage.

应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化，发现药物在一定浓度作用下，DNA指数无明显变化；丹参使C2-M期细胞分布增多，C2-M期延长；川芎嗪使C2-M期细胞分布增多，C2-M期、S期延长；药物使细胞群体倍增时间延长，与浓度呈明显直线正相关。

Specific inhibition of Hh signalling using small molecule inhibitors, a neutralizing anti-Hh antibody or genetic deletion of smoothened in the mouse stroma results in growth inhibition in xenograft tumour models.

由癌细胞分泌的Hedgehog配体，在肿瘤表皮细胞中未能激活信号作用，而是在&基质&上发挥作用（&基质&由细胞外基质、成纤维细胞、内皮细胞和微血管组成，恶性细胞着床于其中）。

Since an excessive cell growth is considered as the main pathological event, cell proliferation model occupy most of the animal models, and these cells include retinal pigment epithelial cell, fibroblast cell, cartilage cell and vascular endothelial cell.

由于PVR的主要病理变化是细胞的过度增生，因而动物模型多以细胞增生模型为主，细胞种类有视网膜色素上皮细胞、成纤维细胞、软骨细胞、血管内皮细胞等。

The methods of sticking tissues piece to culture primary hFCECs and mouse 3T3 fibroblast conditioned medium to culture passage hFCECs were established;2.The Proliferative capacity of corneal limbus hFCECs is better than central hFCECs.3.3T3 conditioned medium could markedly promote the proliferation of epithelial cells

结论一、组织块贴壁法原代培养人胎儿角膜上皮细胞以及小鼠3T3成纤维细胞条件培养液传代培养方法基本建立；二、人胎儿角膜缘部上皮细胞增殖能力优于中央部；三、3T3细胞条件培养液能促进角膜上皮细胞的增殖。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新