成纤维细胞的

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Recently, a newly identified cell type, the fibrocyte, has been reported to contribute to wound healing and fibrotic conditions such as hypertrophic scarring.

近年来，循环成纤维细胞作为一种新型细胞被发现并鉴定，有报道表明这种细胞对创伤愈合和类似瘢痕肥大的纤维变性具有促进作用。

Results With different concentration of sodium arsenite 0.5 ～ 5.0 μmol/L, the skin fibroblasts were markedly stimulated, and a dose effect relationship was observed.

结果与对照组比较，皮肤成纤维细胞经不同剂量的亚砷酸钠诱导后，吸光度值均有改变，存在剂量效应关系（P〈0.01）。0.5～5.0μmol／L浓度的亚砷酸钠对皮肤成纤维细胞有明显的增殖作用，而10μmol／L浓度的亚砷酸钠产生明显的毒性作用。

Hyacinth bletilla decocted water have inhibition effect on fibroblastic activity from rabbit biliary duct.

白及胶对日本大耳白兔胆管成纤维细胞形态的影响及白及胶对日本大耳白兔胆管成纤维细胞活性的抑制作用，可能是白及胶防治日本大耳白兔腹腔粘连的机制之一。

There was obvious difference on fibroblastic activity between control group and Hyacintn bletilla group at 0.1,1.0,10.0 mg/mL on 72 h(P＜0.01,P＜0.05).Conclusion Hyacinth bletilla decocted water have inhibition effect on fibroblastic activity from rabbit biliary duct.

白及胶对日本大耳白兔胆管成纤维细胞形态的影响及白及胶对日本大耳白兔胆管成纤维细胞活性的抑制作用，可能是白及胶防治日本大耳白兔腹腔粘连的机制之一。

On the other hand, extract of cemental matrix was separated and clarified from healthy teeth which were extracted because of orthodontic treatment.

从因正畸需拔除的健康牙体表面获得牙骨质基质提取物，分别观察不同作用时间、不同浓度的牙骨质基质提取物对牙龈成纤维细胞和牙周膜成纤维细胞在牙体表面附着的影响。

Methods Epithelial cells and fibroblast from ameloblastoma, keratocyst, dentigerous cyst, radicular cyst and oral mucosa were cultured and observed by phase-contrast microscope.

原代体外培养成釉细胞瘤细胞、牙源性角化囊肿、根端囊肿及口腔粘膜的上皮细胞和成纤维细胞，倒置光相差显微镜观察。

The cooperation of 8-MOP/UVA could induced dermis of the non- lesion back skin of vulgar psoriatic patients and the cultured dermic fibroblasts showed up photoaging-characteristic changes of histology and biology rapidly, it is feasible to establish human skin photoaging models by selected the human skin of sun-protected sites treatment with PUVA and cultured dermic fibroblasts treatment with 8-MOP/UVA as studied objects.

1.8-MOP/UVA可诱导寻常型银屑病患者背部非皮损区真皮及培养人类真皮成纤维细胞迅速出现具有光老化特征性的组织学及生物学特性改变，以PUVA治疗的非暴露部位皮肤及8-MOP/UVA作用的培养真皮成纤维细胞作为研究对象建立人类皮肤光老化模型是可行的。

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果：①多分化潜能细胞的原代培养情况：胎盘组织消化后获得细胞中仅有少量的贴壁细胞，经两周后逐渐形成扁平单层细胞，呈漩涡状生长或成簇生长，随着细胞密度的增加，胞体变得细长，形态类似成纤维细胞。

RESULTS Expression rate of MHCI antigen of cultured smooth muscle cells and fibroblast was（88.7±4.3）%,（84.8±2.0）%respectively;and that of the revived smooth muscle cells and fibroblasts of aortic homografts after cryopreservation was（89.4±3.5）%,（81.9±2.7）%respectively.

结果 正常培养的大鼠主动脉平滑肌细和成纤维细胞MHCⅠ类抗原表达的阳性率分别为（88.7±4.3）%和（84.8±2.0）%；液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHCⅠ类抗原表达的阳性率分别为（89.4±3.5）%和（81.9±2.7）%。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。