成纤维细胞的

We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料（Hoechst-33342）对核移植体进行染色的方法，对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察，确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

MethodsPhotoaging skin fibroblast models were induced by 8-MOP/UVA. Cordyceps polysaccharides was administrated before 8-MOP/UVA .HE stained,MTT, hydroxyproline, MDA and SOD quantitant were used to test the effects of cordyceps polysaccharides. ResultsCell crimple,condensation of nuclear chromatin in 8-MOP/UVA model group were observed.

方法用8-甲氧补骨脂素联合UVA（8-MOP/UVA）诱导皮肤成纤维细胞光老化，用HE染色、MTT、羟脯氨酸含量、丙二醛含量检测及SOD活力分析等方法观察虫草多糖对成纤维细胞光老化过程中的形态、活力、胶原合成及抗氧化能力的影响。

GM-CSF had dual effects on the cell growth,it could inhibit the fibroblasts proliferation at concentration abov e 1mg/L while stimulate the cell growth at concentration between 1mg/L and 0 .001mg/L.

GM-CSF的浓度在0.001～1mg/L之间对牙周膜成纤维细胞、牙髓成纤维细胞有明显的增殖效应，大于1mg/L时对增殖有明显的抑制作用。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示：支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除，胶原纤维得到了松散，并维持其原有的天然三维网络多孔结构；该材料透水汽性处于3000g.m-2.d-1左右，适合创面恢复；体外降解时间处于25～50h之间，并可根据需要调整工艺条件控制降解时间；拉伸强度介于10.20～11.50MPa之间，具有良好的拉伸强度；收缩温度介于70～85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点，并且其透气性和拉伸强度高、降解性优良且可控，符合组织工程支架材料的要求。

Objective To observe the ultrastructure of supra interspinous ligament of ankylosing spondilitis,to evaluate the role of fibroblasts in ossification of ligament,and to discuss if disorderly arrangement of collagen and sedimentation of calcium granule in AS are induced by fibroblasts.

目的 观察强直性脊柱炎棘上棘间韧带的超微结构和探讨成纤维细胞在韧带骨化中的作用。找出AS韧带中胶原紊乱排列和钙颗粒沉积是否由成纤维细胞活动引起。

Results The endothelial cell and fibroblast of valves and vessel walls in experimental group were completely decellularized, no cell fragments were retained within the matrix scaffold; collagen fiber and elastin fiber had been preserved with intact structure and wavily arrayed; deoxyribonucleic acid content of valves and vessel walls in experimental group were decreased by 97.58%, 97.25% compared with that of control group.

结果 实验组瓣膜及血管壁中内皮细胞、成纤维细胞去除完全，无细胞核碎片；瓣膜及血管壁胶原纤维和弹力纤维呈波浪状排列、整齐，结构完整；瓣膜、血管壁的基因组脱氧核糖核酸含量分别较对照组下降97.58％和97.25％。

Results: The cultured cells showed typical shape and features of fibroblast cell ,which were successfully dissociated and purified.

结果：原代培养的成纤维细胞特征典型，并通过传代培养得到了纯化的成纤维细胞。

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素（bleomycin，BLM）复制大鼠肺纤维化模型，并分离培养肺成纤维细胞，用阿托伐他汀对其进行干预，分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组，用倒置纤维镜观察细胞的形态结构，MTT计数法观察各组细胞的增殖情况，免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ，α-SMA)的表达情况。

Materials and Methods: the HPDLFs were isolated and cultured in vitro from healthy premolars of orthodontic patients, which were 10~14 years old.

材料和方法：取材自临床上10～14岁青少年需要拔除的健康的正畸牙，在体外分离、培养并纯化人牙周膜成纤维细胞，建立人牙周膜成纤维细胞有限细胞系。

MATERIALS: Basic fibroblast growth factor freeze-dry powder (50 mg/ampoule) was thoroughly dissolved with ddH2O to prepare 5 g/L bFGF solution.

材料：取碱性成纤维细胞生长因子冻干粉(50 mg/支)，ddH20彻底溶解，制成含碱性成纤维细胞生长因子 5 g/L的溶液。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新