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Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ~, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞,经传代纯化后,脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞,500μg/mLG418筛选2周,G418减半继续培养2~3代,然后对转染筛选后的细胞进行PCR检测及核型分析。

After then, the phenol and croton oil chemical peeling agents were used to the UVA/B-irradiated skin of hairless mice and untreated group as controls.

同时本实验分别应用酚和巴豆油作用于体外培养原代成纤维细胞,检测成纤维细胞中前体胶原蛋白的表达。

Adult male foreskin subcutaneous tissues apart from epidermis and hypodermis were taken by surgery operation and human foreskin fibroblasts were isolated and cultured, then enhanced green fluorescence protein in human fibroblasts were expressed by lipofectamine and lentiviral vectors, respectively.

取成年男性包皮皮肤的皮下组织,分离培养人皮肤成纤维细胞,分别采用脂质体和慢病毒载体介导转染人皮肤成纤维细胞表达绿色荧光蛋白。

Objective: To Compare the differences in the level of proliferation and apoptosis in keloid fibroblasts and normal skin fibroblasts.

目的:比较瘢痕疙瘩成纤维细胞与正常皮肤成纤维细胞在增殖-凋亡方面的差异。

An attenuated vaccine strain of Muscovy Duck Reovirus, which was suitable for propagating in chicken embryo fibroblast, was obtained by serial passage alternately between Muscovy duck embryo fibroblast and CEF.

应用番鸭胚成纤维细胞和鸡胚成纤维细胞交替传代选育出适应CEF繁殖的番鸭呼肠孤病毒弱毒疫苗株。

Results: The staining of VG, HE show that the subepidermal thickness were reduced , the arrange of collagen tended to uni...

结果:兔耳腹侧面增生性瘢痕局部注射苦参碱后,按时间段取材;VG和HE染色显示真皮层明显变薄,成纤维细胞数量明显减少,胶原排列趋于一致;12周后,对照组苦参碱组的增生指数、成纤维细胞数密度、胶原密度分别为3.25±0.89/2.15±0.12,4517.5±879.6/3551.2±234.1,39.89±14.12/28.75±10.87。

Objective To investigate the expression and significance of basophilic fibroblast growth factor during hepatocarcinigenesis in the rats.

目的 探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)在肝癌发生过程中的表达及意义方法采用免疫组织化学ABC法对二乙基亚硝胺诱发大鼠肝癌发生过程中碱性成纤维细胞生长因子表达情况进行观察。

3There were fusiform cells on the surface of the matrix by SEM, and the cells tightly attach into the membrane.

3扫描电镜下可见PEG/PBU膜表面有梭形的成纤维细胞出现,且成纤维细胞紧密贴附于PEG/PBT膜上。

To observe the influence of hirudin on the cultured sclera flbroblast and extracellular matrix, and research the mechanism of hirudin treating the diseases caused by the proliferous fibroblast.

实验目的通过观察水蛭素对体外培养的巩膜成纤维细胞及细胞外基质的影响,从而探讨水蛭素防治由成纤维细胞增生引起的疾病的机制。

In contrast, hepatocyte growth factor has shown therapeutic effects on injured renal tubules in animal models.

此项研究中,采用人肾脏成纤维细胞培养系统,探讨肝细胞生长因子对高糖诱导的肾间质成纤维细胞损伤产生的作用。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。