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成纤维细胞

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Results In model group, alveolitis increases (much inflammatory cell infiltration), pulmonary interstitial fibroblast increases.

结果]模型组7d时肺泡腔内大量炎性细胞浸润,可见少量成纤维细胞,Ⅰ型细胞受损,Ⅱ型细胞明显增生;28d时肺泡炎减轻,肺间质和胸膜层胶原纤维明显增多。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组),HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显,在第三组二者的变化大致呈平行关系;在第四组基本呈反向变化关系。

Results The endothelial cell and fibroblast of valves and vessel walls in experimental group were completely decellularized, no cell fragments were retained within the matrix scaffold; collagen fiber and elastin fiber had been preserved with intact structure and wavily arrayed; deoxyribonucleic acid content of valves and vessel walls in experimental group were decreased by 97.58%, 97.25% compared with that of control group.

结果 实验组瓣膜及血管壁中内皮细胞、成纤维细胞去除完全,无细胞核碎片;瓣膜及血管壁胶原纤维和弹力纤维呈波浪状排列、整齐,结构完整;瓣膜、血管壁的基因组脱氧核糖核酸含量分别较对照组下降97.58%和97.25%。

Results: The cultured cells showed typical shape and features of fibroblast cell ,which were successfully dissociated and purified.

结果:原代培养的成纤维细胞特征典型,并通过传代培养得到了纯化的成纤维细胞

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果:①多分化潜能细胞的原代培养情况:胎盘组织消化后获得细胞中仅有少量的贴壁细胞,经两周后逐渐形成扁平单层细胞,呈漩涡状生长或成簇生长,随着细胞密度的增加,胞体变得细长,形态类似成纤维细胞

Methods: 24 SD rats were randomly divided into four groups: sham operation group, AD model group, AD model+fibroblasts transplantation groups, AD model+NT4-fibroblasts transplantation group. AD models were made by transecting bilateral fimbria-fornix.

24只雌性SD大鼠随机分为4组:假手术组、AD模型组、AD模型+成纤维细胞移植组和AD模型+NT-4-成纤维细胞移植组。

RESULTS Expression rate of MHCI antigen of cultured smooth muscle cells and fibroblast was(88.7±4.3)%,(84.8±2.0)%respectively;and that of the revived smooth muscle cells and fibroblasts of aortic homografts after cryopreservation was(89.4±3.5)%,(81.9±2.7)%respectively.

结果 正常培养的大鼠主动脉平滑肌细和成纤维细胞MHCⅠ类抗原表达的阳性率分别为(88.7±4.3)%和(84.8±2.0)%;液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHCⅠ类抗原表达的阳性率分别为(89.4±3.5)%和(81.9±2.7)%。

RESULTS Expression rate of MHCI antigen of cult ured smooth muscle cells and fibroblast was (88.7±4.3)%,(84.8±2.0)%respectively; and th at of the revived smooth muscle cells and fibroblasts of aortic homografts after cryoprese rvation was (89.4±3.5)%,(81.9±2.7)%respectively.

结果正 常培养的大鼠主动脉平滑肌细和成纤维细胞MHC Ⅰ类抗原表达的阳性率分别为(88.7±4.3)%和(84.8±2.0)%;液氮低温保存的大鼠主动脉平滑肌和成纤维细胞在复苏后MHC Ⅰ类抗原表达的阳性率分别为(89.4±3.5)%和(81.9±2.7)%。

Materials and Methods: the HPDLFs were isolated and cultured in vitro from healthy premolars of orthodontic patients, which were 10~14 years old.

材料和方法:取材自临床上10~14岁青少年需要拔除的健康的正畸牙,在体外分离、培养并纯化人牙周膜成纤维细胞,建立人牙周膜成纤维细胞有限细胞系。

MATERIALS: Basic fibroblast growth factor freeze-dry powder (50 mg/ampoule) was thoroughly dissolved with ddH2O to prepare 5 g/L bFGF solution.

材料:取碱性成纤维细胞生长因子冻干粉(50 mg/支),ddH20彻底溶解,制成含碱性成纤维细胞生长因子 5 g/L的溶液。

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