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成纤维细胞

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In recent years, bioresearch about ECM indicated that there some kinds of matrix effector cells play key roles in the formation of organic fibrosis and the activation of them is the main source of collagenation cells.

近年来关于ECM的生物学研究表明,在器官纤维化的形成过程中,往往有一些间质效应细胞发挥关键作用,它们的激活已成为胶原生成细胞的主要来源。

Result show:the solution,extracted from rat rail tendons,consist mainly of type I collagen;No growth of bacteria in 37℃ thermostatic culture box for 72 hours;Absorbing the height of ultraviolet-spectrophotometry is 230 nm by using flicker photometer examination;Amino acid in type I collagen consist of aminoacetic acid(40 per cent) and proline(14.3 per cent);Under microscope:collagen gel netted constraction consist of original collagen fibril that had been fairly well-distributed and had a quite regular striate lines ;The collagen solution prepared collagen gel matrixes in 37℃ thermostasis for culture cells and also prepared collagen solid film by washing collagen gel with a phosphate solution for plasty surgery materials.

结果:提取液主要为Ⅰ型胶原蛋白;72小时37℃恒温培养箱内培养无细菌生长;紫外分光光度计测定,吸收高峰为230nm;氨基酸成分主要为甘氨酸:占40%,脯氨酸:14.3%。显微镜下见:相互交织的胶原原纤维结构组成的网状结构;纤维均匀,有规则横纹。胶原蛋白液在37℃恒温下,可制成凝胶,用于培养细胞;用磷酸液冲洗胶原蛋白凝胶可成固体片状,制成胶原膜,用作医用材料。

Histological analysis From day 14 after the operation, osteoblast-like cells appeared in clusters adjacent to vascular sinuses on either side of the fibrous interzone.

组织学分析术后第十四天,在延长部的纤维组织血管窦附近,可见成簇存在的成骨细胞样细胞,并可观察到类骨组织样基质的出现。

Results Fibronectin existed in dental pulp and predentin, and also in space between the wall of dentinal tubule and process of odontoblast in mature dentin. They were mostly distributed in odontoblast layer and round blood vessels and nerves.

结果 纤维粘连蛋白免疫反应普遍存在于牙髓、前期牙本质和成熟牙本质的牙本质小管与成牙本质细胞突起,以成牙本质细胞层,牙髓血管与神经周围最为丰富。

During EMT, the originally cuboidal epithelial cells transform into unanchored, mesenchymal, spindle-shaped, fibroblast-like cells.

当EMT发生时,原来呈立方体的表皮细胞会转变成游离且型态梭状类似纤维组织母细胞的细胞。

Results :①The MVs were formed by osteoblast of the secret type、dentinoblast and process;② There was a mature process of the MVs in the extracellular matrix.The appearance of the hydroxyapatite was a major mature feature;③ HAP formed initially in the MVs entered into collage matrix and eventually mineralized;④ The shifting sign of HAP from MVs to the surface of collage fibril was observed.

结果:①MVs是由分泌型成骨细胞、成牙本质细胞及其突起芽生进入胶原基质;②MVs在细胞外胶原基质中有一个逐渐成熟的过程,并以羟基磷灰石(hydroxyapatite,HAP)晶体的出现为其主要特征;③HAP在MVs内形成、增多,最终进入胶原基质并进一步沉积、钙化;④部分胶原原纤维表面沉积的HAP 晶体与MVs分泌的晶体有明显的移形迹象。

Results Microscopically, the tumor cells were epithelioid or short spindled and special nests of tumor cells separated by arboring fibrovascular septa. Such morphological variants of CCSK as myxoid degeneration, microcysts formation and sclerosing pattern of extracellular hyalined collagen simulating osteoid were present in some regions.

结果 镜下见瘤细胞为上皮样或短梭形,被分枝状纤维血管间质分隔成巢团状,部分区域见黏液样变性微囊肿和细胞外胶原玻璃样变类似骨样组织的硬化型等形态变异。

We describe a case of NLPHL showing a variant histologic pattern characterized by small and large clusters of lymphocyte predominance cells within nodules defined by follicular dendritic cell meshworks, associated with numerous neutrophils and areas of internodular fibrosis. Focally, the neoplastic cells had lacunar cell-like cytologic features.

在此,我们描述一例组织形态特殊的NLPHL,其形态学表现为以淋巴细胞为主的细胞排列成大小不等的结节,结节均由滤泡树突状细胞网网罗,结节内见中性粒细胞浸润,部分结节间见纤维化,局灶瘤细胞呈陷窝样。

Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达:取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例,其中骨旁骨肉瘤4例,纤维母细胞型骨肉瘤13例,软骨母细胞型骨肉瘤12例,骨母细胞型骨肉瘤26例,切成3μm的组织切片备用。

Results Fibronectin existed in dental pulp and predentin, and also in space between the wall of dentinal tubule and process of odontoblast in mature dentin. They were mostly distributed in odontoblast layer and round blood vessels and nerves. In the crown pulp, integral optical density, length density and volume density were 0.49±0.33,38.44±20.7 and 24.56±10.8 respectively.

结果 纤维粘连蛋白免疫反应普遍存在于牙髓、前期牙本质和成熟牙本质的牙本质小管与成牙本质细胞突起,以成牙本质细胞层,牙髓血管与神经周围最为丰富。

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