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成纤维细胞

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Objective: To observe the effects of Cinnamyl aldehyde on NIH3T3 cell cycle and explore the possible mechanism further.

中文摘要:目的:观察肉桂主要成分桂皮醛对小鼠成纤维瘤细胞株NIH3T3细胞周期及相关蛋白的影响。

In order to test this hypothesis, we isolated and developed stable self-renewable cell lines of mesenchymal stem cells from human fetal articular cartilage and identified the cell phenotype by FACS and we found that their phenotypic markers were identical to those of mesenchymal stem cells from bone marrow.

我们从胚胎的关节软骨中获得成纤维样细胞,在体外进行培养和扩增,并用流式分析的方法对其表面标志进行鉴定,发现其表面标志与文献报道的骨髓来源的间充质干细胞的表面标志相同。

Pathological diagnosis as follows: Chon- droblastoma (3 cases), giant cell tumor of bone (3 eases), fibrous dysplasia (2 cases), non-ossifying fibroma (1 case), and esosinophilic granuloma of bone (1 case).

材料和方法:经手术病理和临床活检证实共10例,其中成软骨细胞瘤和骨巨细胞瘤各3例,骨纤维异常增殖症2例,非骨化性纤维瘤和嗜酸性肉芽肿各1例。

Recombinant apoE〓 protein could inhibit the death of the neurocyte cell of mice brain induced by beta amyloidal peptide 25-35 fragment and the death of the SK-N-SH-neuroblastoma cell induced by beta amyloidal peptide 25-35 fragment and 1-40 fragment.

重组apoE〓蛋白对Aβ 25—35多肽诱导的胎鼠大脑皮质神经细胞凋亡及Aβ25-35和A β1-40多肽诱导的神经成纤维胶质瘤细胞生存力下降均具有明显的拮抗作用,表明apoE对神经退化性疾病中的细胞死亡具有拮抗作用。

Fibrillar collagen(Collagen typeⅠand Ⅲ) in sinusoids of normal liver was successfully observed through second harmonic generation microscopy.

分别用前向二次谐波及背向双光子荧光对肝脏标本的成纤维胶原和肝细胞浆进行成像,将结果与传统的Masson's三染色法进行比较。

Objective To study the role of fibroblastic stromal cells in aseptic loosening.

目的 研究成纤维母细胞样基质细胞在人工关节置换术后无菌性松动中的作用。

Many hES cell lines have been established under different conditions in the world. The firstly established hES cell lines were cultured on mouse embryonic fibroblast cells with medium containing many undefined animal components such as fetal bovine serum, which may cause cross-transfection with animal pathogen and mycoplasm.

目前,世界上已经建立了多株hES 细胞系,最早建立的hES 细胞系是生长在小鼠胚胎成纤维(mouse embryonic fibroblast, MEF)细胞上的,培养体系中含血清等动物源性成分,这些成分可能引起动物源性病原体或支原体的污染,从而限制了 hES 细胞的临床应用。

The totipotency of ESCs was identified by the observation of cells' morphology and the formations of teratoma in immunocomprised mice.

结果:(1)体外培养的小鼠ESC-D3细胞在胚胎成纤维饲养层细胞上连续传代培养,仍保持向3胚层分化的能力。

Methodshexamethyldisiloxane polymer film was obtained by plasma polymerization method on coverslips. oxygen (o2) plasma treatment was employed to modify the surfaces with different time so that a series of surface with different wettability were produced. l929 cell were cultured on these surfaces to observe the cell attachment rate and cell spreading in 6 and 12 hours.

使用薄膜等离子体聚合法在圆形盖玻片表面生成六甲基二硅氧烷(hexamethyldisiloxane, hmdso)薄膜聚合体,然后用低温氧等离子体轰击聚合体表面,根据轰击时间的不同形成一系列的不同润湿性表面,将成纤维细胞系l929接种于不同润湿性表面,观察6h、12h时细胞的贴附率,并观察细胞的形态。

Methods Hexamethyldisiloxane polymer film was obtained by plasma polymerization method on coverslips. Oxygen (O2) plasma treatment was employed to modify the surfaces with different time so that a series of surface with different wettability were produced. L929 cell were cultured on these surfaces to observe the cell attachment rate and cell spreading in 6 and 12 hours.

使用薄膜等离子体聚合法在圆形盖玻片表面生成六甲基二硅氧烷(Hexamethyldisiloxane, HMDSO)薄膜聚合体,然后用低温氧等离子体轰击聚合体表面,根据轰击时间的不同形成一系列的不同润湿性表面,将成纤维细胞系L929接种于不同润湿性表面,观察6h、12h时细胞的贴附率,并观察细胞的形态。

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