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成纤维细胞

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"You can transfect a gene into a fibroblast," he said.

你可以将基因转染进成纤维细胞&。&

Objective To search for the best approaches to transfect plasmid pEGFP-N1 into human fibroblasts.

目的 采用3种不同的方法将质粒pEGFP-N1转染人成纤维细胞,从中寻找最佳的稳定转染的方法。

Results: it was efficient to transfect chicken embryonic fibroblast cells by the method of lipofectamine mediation. the expression levels of protein were significantly different.

结果 脂质体介导的方法能有效转染鸡胚胎成纤维细胞,实验中egfp蛋白相对表达量随质粒量的增加而明显升高(p.01)。

AIM: To observe the influence of recombinant human relaxin on cardiac fibroblast functions under angiotensin II.

目的: 观察在重组人松弛素及血管紧张素Ⅱ作用下对心脏成纤维细胞功能的影响。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代,取第5、10、15和20代重组病毒进行以下检测:用蓝斑试验鉴定各代重组病毒纯度;用PCR方法扩增猪瘟病毒E0、E2基因,进行基因序列分析;用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

"Second, both solid tumors and ascular anomalies (e.g., hemangiomas, port-wine stains) are known to hae increased expression of angiogenic proteins, including ascular endothelial growth factor and basic fibroblast growth factor."

&第二,已知实体瘤和脉管异常(例如血管瘤、葡萄酒色痣)存在血管原的蛋白表达增加,包括血管内皮生长因子和碱性成纤维细胞生长因子。&

OBJECTIVE: To observe the effect of acupotomology intervened third lumbar transverse process syndrome rat on levels of the fibroblast proliferation.

目的:观察针刀疗法对第三腰椎横突综合征模型大鼠组织中成纤维细胞增殖的影响。

Objective: To establish a 3D culture model of fibroblasts.

目的建立一种成纤维细胞的三维培养方法。

OBJECTIVE: To observe the influence of nerve growth factor on the biological characteristics of scar fibroblasts.

目的:观察神经生长因子对瘢痕成纤维细胞生物学特征的影响。

Objective: To establish a simple method for isolation and culture of mouse tail-tip fibroblasts .

目的:建立一种简单的鼠尾成纤维细胞分离、培养的方法。

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