成纤维细胞

We also confirmed in this paper that it is injected somatic cells but not broken cytoplasm formed multi blastomere through mitosis in the reconstructed embryos.

在本实验中还通过用DNA染料（Hoechst-33342）对核移植体进行染色的方法，对融合后成纤维细胞在核移植体内不同时间的运行及发育模式进行了观察，确认了是成纤维细胞经有丝分裂形成多个卵裂球而不是由于细胞质碎裂形成的两个或多个碎块。

Periostin may affect the function of fibroblasts of hyperplasic scars. periostin may be a cicatrix specific gene.

periostin在过度增生性瘢痕成纤维细胞中的表达增高，氢化可的松可能抑制其基因的表达；periostin可能影响过度增生性瘢痕成纤维细胞的功能，它可能是一种瘢痕相关基因。

MethodsPhotoaging skin fibroblast models were induced by 8-MOP/UVA. Cordyceps polysaccharides was administrated before 8-MOP/UVA .HE stained,MTT, hydroxyproline, MDA and SOD quantitant were used to test the effects of cordyceps polysaccharides. ResultsCell crimple,condensation of nuclear chromatin in 8-MOP/UVA model group were observed.

方法用8-甲氧补骨脂素联合UVA（8-MOP/UVA）诱导皮肤成纤维细胞光老化，用HE染色、MTT、羟脯氨酸含量、丙二醛含量检测及SOD活力分析等方法观察虫草多糖对成纤维细胞光老化过程中的形态、活力、胶原合成及抗氧化能力的影响。

After then, the phenol and croton oil chemical peeling agents were used to the UVA/B-irradiated skin of hairless mice and untreated group as controls.

同时本实验分别应用酚和巴豆油作用于体外培养原代成纤维细胞，检测成纤维细胞中前体胶原蛋白的表达。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

The microstructure of the scaffold was observed under photomicroscope and scanning electron microscopy,and the in vitro degradation time,permeability,tensile-strength,porosity,shrinka...

实验结果显示：支架中的成纤维细胞、脂肪细胞及组织纤维间质完全去除，胶原纤维得到了松散，并维持其原有的天然三维网络多孔结构；该材料透水汽性处于3000g.m-2.d-1左右，适合创面恢复；体外降解时间处于25～50h之间，并可根据需要调整工艺条件控制降解时间；拉伸强度介于10.20～11.50MPa之间，具有良好的拉伸强度；收缩温度介于70～85℃之间。上述结果表明该材料已解决了猪脱细胞真皮基质渗透性差、降解速度过慢的缺点，并且其透气性和拉伸强度高、降解性优良且可控，符合组织工程支架材料的要求。

Methods: Fibroblast cell were treated with -300,-500 and -1 000V PTFE electrets for 24, 48 and 72 h, respectively, and the influence of negative electrets on cell apoptosis was studied by means of flow cytometry and transmission electron microscope.

选用-300、-500和-1 000 V PTFE驻极体作用于成纤维细胞24、48和72 h，利用流式细胞仪和透射电子显微镜研究负极性驻极体对成纤维细胞凋亡的影响。

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素（bleomycin，BLM）复制大鼠肺纤维化模型，并分离培养肺成纤维细胞，用阿托伐他汀对其进行干预，分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组，用倒置纤维镜观察细胞的形态结构，MTT计数法观察各组细胞的增殖情况，免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ，α-SMA)的表达情况。

RESULTS: In the verapamil group, the fibroblast were round or oval, and there were tiny cell process, less alpha substance, atrophic and decreased rough endoplasmic reticulum in kytoplasm and abundant vesica. In control group, there were fusiform or polygon fibroblast, and much matrix among cells, abundant rough endoplasmic reticulum and ribosome in cytoplasm and less vesica were found.

结果：维拉帕米组神经瘢痕成纤维细胞呈球形或椭圆形，细胞突起细小，胞间网状物质少，胞浆中粗面内质网萎缩且数量减少，囊泡丰富；对照组成纤维细胞呈梭形或多角形，细胞间基质较多，细胞浆内粗面内质网及核糖体丰富，囊泡少见。

In contrast, hepatocyte growth factor has shown therapeutic effects on injured renal tubules in animal models.

此项研究中，采用人肾脏成纤维细胞培养系统，探讨肝细胞生长因子对高糖诱导的肾间质成纤维细胞损伤产生的作用。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。