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成纤维细胞

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RESULTS: BMP-2 was mainly found in the plasma of fibroblast, osteoblast, cementocyte, odontoblast separately.

结果:牙周膜成纤维细胞、成骨细胞、成牙骨质细胞及胶原纤维中均可见BMP-2阳性染色。

AFGF shows greatpotential for clinical applications for therapy of a variety of diseases, such as Parkinson'sdisease, spinal cord contusion injury, neural regeneration in reimplantation of broken-offfinger, brain ischemia, renal ischemia, myocardial infarction, occlusive vascularitis, retinalischemia, gastric ulcer and nonhealing wound and so on. We expressed human acid fibroblast growth factor gene transiently in tobacco byAgro-bacterium mediated vacuum infiltration and studied the factors, which influenced theexpression level.

人类酸性成纤维细胞生长因子(aFGF,FGF-1)是成纤维细胞生长因子超家族成员,来自于三个胚层的多种细胞都可以表达aFGF.aFGF在治疗帕金森综合症、急性脊柱扭曲性损伤、断指中神经功能重建、脑缺血、肾缺血、心肌梗塞、闭塞性脉管炎、视网膜缺血、胃溃疡及难愈合性伤口等多种临床应用方面具有巨大潜力。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM;(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原;(3)用0.02%戊二醛溶液浸泡ADM5-10min,制成交联型ADM;另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h,制成胶原包埋型ADM,冰冻保存;(4)用酶消化法培养原代成纤维细胞,取第三代增殖期细胞,将其调整至浓度为2×105/ml的成纤维细胞悬液。

AIM: To investigate the effects of dibairen decoctum on the proliferation of keratinocytes and fibroblasts as well as the potential effects of DBRD on fibroblast contraction.

目的:观察地白忍煎剂对角朊细胞和成纤维细胞增殖的影响以及对成纤维细胞收缩功能的潜在作用。

Adding dividedly the solution of two drugs with different concentration into the heperplastic scar fibroblasts and keloid fibroblast which were in logarithmic growth phase. After 48 hours in vitro culture, hydroxyproline colorimetric test was conducted, then statistics comparison was carried out in accordance with hydroxyproline content which was calculated based on OD value.

向对数生长期的增生性瘢痕成纤维细胞及瘢痕疙瘩成纤维细胞中加入不同浓度的两种药液,培养48小时后进行羟脯氨酸比色试验,并将 OD 值依公式换算成羟脯氨酸含量,进行统计学比较。

Extraperiosteal ossification of bone injury induced by fluoride is closely associated with fibroblasts which belong to inducible osteogenic precursor cell in bone, they have osteogenic function under special conditions.

氟性骨损伤骨周化骨与成纤维细胞密切相关,在骨周组织中,成纤维细胞(fibroblast, FB)属于诱导性骨祖细胞范畴(inducible osteogenic precursor cell, IOPC),在特殊条件下具有成骨功能。

The study was done to compare the induction efficiency of human embryonic stem cells to neuroepithelial progenitor cells in human embryonic fibroblasts,mouse embryonic fibroblasts and feeder free systems.

中文摘要:[摘要]比较人胚胎干细胞在人胚胎成纤维细胞、鼠胚胎成纤维细胞饲养层和无饲养层3个诱导体系下形成神经上皮祖细胞的能力。

To observe the influence of hirudin on the cultured sclera flbroblast and extracellular matrix, and research the mechanism of hirudin treating the diseases caused by the proliferous fibroblast.

实验目的通过观察水蛭素对体外培养的巩膜成纤维细胞及细胞外基质的影响,从而探讨水蛭素防治由成纤维细胞增生引起的疾病的机制。

Coculturing of the transfected fibroblasts with neonatal rat ventricular myocyte cultures resulted in a significant reduction (68%) in the spontaneous beating frequency of the cultures compared with baseline values and cocultures seeded with naive fibroblasts.

与基线值和原始的成纤维细胞的再复制相比,乳鼠心室肌细胞培养基里转录的成纤维细胞的再繁殖,导致了培养基里自律性频率的显著下降(68%)。

Coculturing of the transfected fibroblasts with neonatal rat ventricular myocyte cultures resulted in a significant reduction (68%) in the spontaneous beating frequency of the cultures compared with baseline values and cocultures seeded with naive fibroblasts.

结果发现,与基线、与幼稚成纤维细胞共培养相比,转染的成纤维细胞和新生大鼠心室肌细胞共培养导致培养物自发搏动频率的下降(68%)。

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