成纤维细胞

METHODS: Detection of the cell proliferation by MTT method and ALP expression by enzyme dynamics method.

原代培养牙髓成纤维细胞、牙周膜成纤维细胞，用MTT法测定细胞增殖，酶动力学方法测定ALP活性。

Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中，我们在相同条件下，将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells，HEL)和3株传代细胞株：人肝癌细胞(Human hepatic carcinoma 3B cells，Hep-3B)；人肺腺癌细胞(Human lung carcinoma A549 cells，A549)；鼠正常成纤维细胞(Normal mouse fibroblastscells，NIH 3T3)。

Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

A strain of new virus was isolated from fledgling budgerigars by authors in 1995. Cytopathic effect became visible after the isolate was passaged 4 generations on budgerigar embryo fibroblast and 5 generations on Chicken embryo fibroblast.

摘 要 1995年作者从湖北濒死鹦鹉体内分离到一株新病毒，经鹦鹉胚成纤维细胞盲传四代过渡到鸡胚成纤维细胞，再传至第五代出现细胞病变。

OBJECTIVE: To study the regulation of bFGF on the blast-colony-forming cell by adding bFGF in the medium of embryoid body generation.

目的：在拟胚体培养阶段施加碱性成纤维细胞生长因子，验证碱性成纤维细胞生长因子对集落形成细胞产生的调控作用。

Methods: CF were isolated and cultured from Neonatal SD rats.The cells were divided into different groups and treated with Ang-(1-7), TGF-β1, TGF-β1+ Ang-(1-7), and the acceptor inhibiter of TGF-β1 respectively.

分离培养SD仔鼠心脏成纤维细胞，以Ang-(1-7)、TGF-β1、Ang-(1-7)+TGF-β1组、TGF-βⅠ型受体抑制剂等干预，通过WST-1法检测细胞增殖，RT-PCR测定心脏成纤维细胞Smad3、Smad 7mRNA的表达。

By the cooperation of 8-MOP/UVA, the photoaging-characteristic biological markers of the dermic fibroblasts in vivo and in intro were changed as follows:① with a permanent switch of mitotic to stably postmitotic phentypes, fibroblasts displayed growth suppression and morphological changes of cell senescence;② increasing expression of SA-β-galactosidase;③ increasing expression of p16 protein;④ continuous up-regulation of mRNA of MMP-1 and MMP-3, while the protein of TIMP-1 was only slightly induced;⑤ high levels of the 4977bp deleted mtDNA accumulated in dermis and cultured fibroblasts, and large accumulation of a A→C base transversion of 414 position of 〓 of human mtDNA control region for replication of cultured fibroblasts also.

2.8-MOP/UVA作用下，体内外真皮成纤维细胞生物学特性出现具有光老化特征性的改变：①细胞由具有分裂活性的分裂表型转化为不具有分裂能力的分裂后表型，形态学出现细胞衰老的相应改变；②SA-β-Gal表达增加；③p16蛋白表达增加；④MMP-1、MMP-3 mRNA持续表达而TIMP蛋白表达仅被轻微诱导；⑤mtDNA 4977bp缺失大量累积，培养成纤维细胞mtDNA复制控制区〓片段414碱基迅速出现大量A→C的点突变。

OBJECTIVE: To compare the differences of gene expression and the response to bFGF of human umbilical cord CD34+ and CD133+ cells, and to explore gene expression changes of bFGF-induced umbilical cord CD34+ and CD133+ hematopotic stem cells/hemapoietic progenitor cells in vitro.

目的：利用基因芯片比较脐血CD34+和CD133+细胞基因表达的差异，并探讨碱性成纤维细胞生长因子体外诱导脐血CD34+和CD133+造血干/祖细胞分化的基因表达变化，及其对碱性成纤维细胞生长因子反应性差异。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果：从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs，该细胞可传25代以上，且经过传代及冻存复苏后生物学特性无改变；hEFs表达脯氨酰4-羟化酶β亚单位，不表达细胞角蛋白4，确定其为成纤维细胞；该成纤维细胞表达bFGF和LIF。

Following successful modeling, rats of bFGF group were intratracheally injected with 400 U bFGF and rats of VEGF group with 2 μg VEGF, once a week for three times. MSCs group was injected 1 mL suspension of 4×109/L MSCs into tail vein. MSCs+VEGF group was injected MSCs into tail vein and intratracheally injected VEGF (2 ug, three times) at the same time. Model control and normal control groups were intratracheally injected with equal volume of sodium chloride.

成功造模后，碱性成纤维细胞生长因子组气管内注入400 U碱性成纤维细胞生长因子，血管内皮生长因子组气管内注入2 μg血管内皮生长因子，1次/周，共3次；单纯细胞移植组于尾静脉注入4×109 L-1骨髓间充质干细胞悬液1 mL；血管内皮生长因子+细胞移植组气管内注入血管内皮生长因子的同时，尾静脉注入骨髓间充质干细胞；模型对照组、正常对照组给予相同体积的生理盐水。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。