成纤维细胞

The endothelial cells cocultured with fibroblasts from scar had greater proliferation rate than that from normal skin.

另外，Transwell 共培养结果发现，增生性瘢痕来源的成纤维细胞具有显著促进血管内皮细胞增殖的作用。

To explore the reciprocal effects of host-tumor interaction, we developed a system to assess the gene expression patterns and the diversity of gene expression of ERK and PI3K pathway in CAOV3 human ovarian cancer cells and primary carcinoma-associated fibroblasts cocultured with each other for 48 hours with RT-PCR.

方法1。采用逆转录聚合酶链反应的方法分别检测交互作用48小时后的卵巢癌相关成纤维细胞与卵巢癌细胞中基因表达的改变，CAFs及CAOV3细胞外信号调节激酶通路的ERK，PI3K基因表达的变化；2。

RESULTS: Stable telomerase activity could be detected in the transfected fibroblasts and no signs of cell senescence were found in the fibroblasts cultured for more than 50 doublings.

结果 转染 h TERT的人成纤维细胞能稳定表达端粒酶活性，培养超过 5 0代后细胞仍保持β-半乳糖苷酶阴性。

To further elucidate the mechanism of BMP in regulating cell differentiation and to provide basis for BMP gene therapy, this study centers on the effects of BMP2 gene transfection on fibroblasts and contains the following works:① A 1.2kb fragment containing full length human BMP2 coding region was inserted into pBK-CMV (expressed both in protocaryon and eukaryon) by using predetermined orientation methods, resulting in phagemid expression vector for BMP2 (pBK-B2).② pBK-B2 was transfected into NIH3T3 cells by using LipofectAMINE.

为了进一步从分子水平探讨BMP对细胞分化的调控作用，并为BMP基因疗法的建立提供依据，本实验围绕BMP2基因转染对成纤维细胞的生物学作用进行了以下几个方面的工作：①将含有人BMP2全长编码序列的基因片段(1.2kb)定向克隆于噬菌粒表达载体pBK-CMV中，成功构建了人BMP2噬菌粒表达载体pBK-B2。

We used the NIH3T3 fibroblast cells ascarrier because the cell clone is easy to expand,to betransfected and to express the exogenous gene.In the presentstudy,a model of fibro blast cell-mediated human IFN-〓 genetherapy was established and its anti-tumor activity was observed.

为此，我们选择了易于扩增、易于转染并能稳定表达外源目的基因的成纤维细胞为载体细胞，建立了人〓基因疗法的实验模型，并观察了其体内抗肿瘤作用。

Healing wounds in Lsp1 mice had higher densities of neutrophiles, macrophages, and fibrocytes.

LSP1缺乏小鼠的愈合创面中有更高密度的欧天芥菜碱、巨噬细胞以及循环成纤维细胞的积聚。

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞，原代培养2d后改变培养基，添加含20%胎牛血清的DMEM（含25ml/L葡萄糖，100U/ml青链霉素，pH值7.6），添加烟酰胺10mmol/L，胰岛素1mg/L，N2添加剂，碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

Fluid nitrogen cryopreservation has no obvious effect on the MHC antigeniacity of aortic homografts of rats.

液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞MHC类的抗原性没有明显的影响。

In the rat carotid injury model, SM16 dosed once daily orally at 15 or 30 mg/kg SM16 for 14 days caused significant inhibition of neointimal thickening and lumenal narrowing. SM16 also prevented induction of adventitial smooth muscle alpha-actin-positive myofibroblasts and the production of intimal collagen, but did not decrease the percentage of proliferative cells.

在大鼠颈动脉损伤模型中，每日口服15或30mg/kgSM16持续14天能够显著抑制新生内膜增厚及管腔狭窄，预防对外膜α-SMA阳性肌成纤维细胞的诱导和内膜胶原的合成，但并不降低增殖细胞的比例。

BACKGROUND: Basic fibroblast growth factor can accelerate the bone marrow mesenchymal stem cells proliferation and differentiation into nerve cells, which is considered as a mitogen of glial cells.

背景：碱性成纤维细胞生长因子可以促进骨髓间充质干细胞的增殖和向神经细胞方向分化，并被认为是胶质细胞的分裂原。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。