成纤维细胞

ZHAO Yede*,HE Qinglian.Department of Plastic Surgery, Navy 411 Hospital, Shanghai 200081Objective To investigate the expression level and intracellular distribution of calreticulin in hypertrophic scar-derived fibroblastsand normal skin-derived fibroblasts.

增生性瘢痕(Hypertrophic scar， HS)作创伤及外科手术后常见且十分棘手的皮肤组织疾病，瘢痕组织内成纤维细胞异常活化和细胞外间质过度沉积既是HS的重要组织学特征，也是HS发生、发展的物质基础。

The third passage of both HCF and HPF were measured by 3-(4,5-dimethyl-2thiazol)-2,5-diphenyl-2H-tetrazolium bromid at various days of culture.

对传第3代的两种成纤维细胞，分别于接种后2、5、8d行噻唑蓝[3-(4，5-dimethyl-2thiazol)-2，5-diphenyl-2H-tetrazolium bromid， MTT]比色试验，检测细胞增殖活力。

To isolate, cultivate and identify the human embryonic fibroblasts derived from the gonadal ridges and dorsal mesenteries of human embryos, and to detect the expression by hEFs of cytokines crucial for the growth of human embryonic germ cells in vitro.

目的：从人胚胎生殖腺嵴和肠背系膜中分离、培养及鉴定人胚胎成纤维细胞，检测hEFs表达人胚胎生殖细胞体外生长所需的重要细胞因子。

The hEFs derived from gonadal ridges and dorsal mesenteries of human embryos are successfully isolated and cultivated,and the cells express the cytokines essential for the growth of hEG in vitro.

成功地分离和培养了人胚胎生殖腺嵴和肠背系膜来源的成纤维细胞，证明其表达对于hEG体外生长起重要作用的细胞因子。

Primordial germ cells derived from gonadal ridges and mesenteries of 30-70-day post menopause human ernbiyos, when cultured on mouse MEF or STO fibroblast feeder layers in presence of hrLW , hrbFGF, hrSCF, and forskolin could survive and give rise to multicellular colonies, resembLing mouse ES-like colonies .

从停经30-70天流产的早期胚胎后肠系膜中或生殖嵴部位分离到的原始生殖细胞，在小鼠的原代MEF或STO成纤维细胞等饲养层细胞上培养，能在hrLIF、hrFGF、hrSCF、forskolin等的共同作用下存活并增殖。

Ultrastructure revealed that endotheliocytes were tumefied and destructured, some blood vessels lost functions and proliferation and secretion of fibroblast reduced markedly.conclusion ad-meth1 has marked inhibitory function on the form of hypertrophic scar.

结果：ad-meth1注射后30天，兔耳瘢痕组织学染色显示微血管分布明显减少，超微结构显示内皮细胞肿胀、变性，部分血管丧失功能，成纤维细胞增殖及分泌活性明显降低。

METHODS: BMSCs were obtained from Japanese big-eared rabbits, and in vitro cultured. Then the subcultured BMSCs were transfected by pCDNA3.1 plasmid, followed by incubation on swine SIS to construct the tissue-engineered skin. The growth of cells and phenotype of BMSCs were detected by flow cytometry.

日本大耳兔骨髓间充质干细胞进行体外培养扩增后，通过pCDNA3.1质粒将碱性成纤维细胞生长因子基因转染至生长状态良好的骨髓间充质干细胞，并将转染后的细胞接种于制备好的猪小肠黏膜下层上，进行体外联合培养，构建组织工程皮肤。

Fibroblast growth factor 2; cell hypoxia ; cobalt chloride

成纤维细胞生长因子2；细胞低氧；氯化钴

On base of this, we investigated the effect of the physical property of scaffolds and fibroblast coculture on hepatic differentiation of hADSCs.

在此基础上进一步探讨PLGA支架物理特性和成纤维细胞共培养对人脂肪干细胞向肝细胞分化的影响。

Effect of tubular epithelial cells activated by aristolochic acid on renal interstitial fibroblasts in coculture system.

通过共培养观察被马兜铃酸活化后的肾小管上皮细胞对成纤维细胞的作用。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。