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成纤维细胞

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Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞,G418抗性筛选3-4周后,经PCR扩增和报告基因EGFP表达检测,得到稳定整合外源基因的转基因供体细胞系,为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

Objective To investigate the role of endothelin-1 and its receptors on hypertrophy or proliferation of cultured cardial cells.

目的 观察内皮素-1(ET-1)及其受体对心肌细胞肥大和心脏成纤维细胞增殖的作用。

It was found that the extract of Ficus carica leaves showed remarkable inhibitory and killing effects on the Newcastle disease virus.

采用组织细胞培养技术,充分利用无花果叶提取物中的有效成分,在人上皮样癌(Hep-2)细胞株、地鼠肾(BHK21)细胞株和鸡胚成纤维细胞上研究其抗新城疫病毒的作用。

Objective: To observe the attachment and growth of fibroblasts on a new porcine collagenic dermal matrix and investigate a method to improve the cell compatibility of the porcine dermal scaffold.

目的:观察成纤维细胞在一种新型的猪胶原基真皮支架上的粘附生长特性,探讨提高异种真皮支架细胞相容性的方法。

Objective To evaluate the cytotoxicity and cell affinity of a porcine collagenic dermal matrix to guide the improvement of the porcine dermal scaffold.

目的 通过对一种新型的猪胶原基真皮支架的细胞毒性和与成纤维细胞的亲和性进行评价,为这种新型生物材料的安全应用及工艺改良提供依据。

2The distribution of HIF-1 a and COX-2 was unequal;.(3)These findings indicate that HIF-1a and COX-2 may be two important factors in pulpal lesions and there is a significantly hypoxia condiction in inflamed pulp .(4)The increase of HIF-1 a is a possible mechanism for hypoxia preconditioning .(5)Our results suggested that pulpal fibroblasts as well as macrophages might be involved in the pathogenesis of irreversible pulpitis.

和COX一2的表达均有不同程度的变化,在牙髓炎病程中可能发挥着重要的生理和病理作用,③在牙髓炎进程中和牙髓自身修复过程中存在着缺氧环境和缺氧耐受机制,④牙髓成纤维细胞有可能与单核巨噬细胞一同参与牙髓炎的病理进程,且具有修复和免疫功能。

Objective To investigate the best method of culture conjunctival epithelial cells and fibroblasts in vitro , and further more to provide a foundation for investigating its biologic feature and reconstruction.

目的 探讨体外培养兔眼结膜上皮细胞和成纤维细胞的最佳方法,为进一步研究结膜的生物学特性和结膜重建提供基础。

Results After used the SGS , the arrange of collagen tend to unifying , micrangiums are rich and in opening condition, the polymeride of pre-collagen accumulate in cytochylema in fibroblast, the total content of collagen is decreased, but the content of hPcⅢ is kept in a high level, the content of HA is increased gradually,which closes to the normal skin level.

结果 应用硅凝胶膜后,胶原排列趋于一致,微血管丰富而呈开放状态,成纤维细胞的前胶原聚合体蓄积在细胞浆内;胶原总的含量减少,但Ⅲ型前胶原含量维持在一个较高水平;透明质酸含量逐渐升高,接近正常皮肤水平。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象,采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法,研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

Basic fibroblast growth factor ; Endothelia cell ; NF-κB ; ki-67; Proliferation

碱性成纤维细胞生长因子;内皮细胞; NF-κB ; ki-67;增生

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Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

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