成纤维细胞

The transformed cell could be agglutinated by lower concentration of ConA and could grow in semisolid agar.

为了探讨三氧化二镍(Ni2O3)诱导人肺成纤维细胞恶性转化作用以及β-胡萝卜素和维生素C的保护作用，用不同浓度的Ni2O3在体外多次处理HLF细胞，利用刀豆凝集素A凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定；同时添加β-胡萝卜素(5.4mg/L)和维生素C的食物(1.8mg/L)，观察对Ni2O3转化作用的影响。

The results showed that 4-5 weeks after infection, only minimal granulomas were observed which consisted largely of eosinophils and lymphocytes.

结果发现，在感染后4～5周的未成熟虫卵周围，主要组成细胞是嗜酸性白细胞，其次是淋巴细胞、巨噬细胞和成纤维细胞。

After natural purification and three passaging, we detected CD90 expression in fibroblasts grown on cover glass by SABC immuocytochemistry techniques.

经过3次传代、自然纯化后得到性质稳定的成纤维细胞，冻存后复苏传1-3代，制成细胞爬片，免疫细胞化学SABC法染色检测CD90的表达情况。5。

Methods Human and pig dermal fibroblasts were isolated from the skin by enzymation and cultured in vitro. The second passage of human and pig dermal fibroblasts were collected and seeded at a density of 1.0×10^6 cells/mL onto cylinder-shaped polyglycolic acid scaffolds.

酶消化法获取人与猪皮肤成纤维细胞，培养扩增，取第2代细胞，以1×10^6/mL的细胞密度接种至聚羟基乙酸圆柱形支架上体外培养。

the possible mechanism of antiaging effect of fructus corni polysaccharides is to regulate the expression of cyclind1 and cdk4 in hdf senescence model cell.

山茱萸多糖可能通过改变细胞周期调控因子的表达而发挥其抗hdf细胞衰老作用。山茱萸多糖；cyclind1；cdk4；人二倍体成纤维细胞

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

Reverse transcription polymerase chain reaction and western blot were used to detect the COCH and POU3F4/brn4 gene's expression. To investigate in vitro characteristics of COCH gene's expession, two factors were introduced in the culture medium of spiral ligament type Ⅰ fibrocytes respectively. Firstly, phosphorothionate-modified antisense oligodeoxynucleiotides to POU3F4/brn4 mRNA was applied to study the regulation effects of POU3F4/brn4 to COCH gene transcription.

引入人工合成的针对POU3F4／brn4基因mRNA的反义硫代磷酸寡聚脱氧核苷酸，干扰耳蜗螺旋韧带Ⅰ型成纤维细胞POU3F4／brn4的表达，免疫印迹检测反义干扰细胞POU3F4／brn4蛋白条带的强弱，RT-PCR检测反义寡核苷酸干扰细胞COCH基因转录水平的变化，观察POU3F4／brn4对COCH基因的转录调控作用。

For the purpose of constructing mammary gland specific expressional vector, we use the updream regulation sequence of bovine BLG to regulate lacS gene to express in mammary gland. Positive clone cells are selected and purified by the expression Neo gene and report gene EGFP. So the cells can be used as sources to create transgenic animals by nuclear transfer techniques.

本研究以牛β-乳球蛋白基因上游调控成分作为启动子，嗜热菌糖苷酶基因lacS为目的基因，构建了lacS基因乳腺特异表达载体，并通过脂质体介导法转染体外培养的牛胎儿成纤维细胞，借助新霉素抗性基因和增强型绿色荧光蛋白报告基因来筛选、纯化阳性克隆细胞，为利用核移植技术制备转基因动物提供细胞来源。

Objective: Basic Fibroblast Growth Factor is a mitogen for many cell types, including osteoblasts and chondrocytes. It can stimulate angiogenesis and oateoblast gene expression.

目的：碱性成纤维细胞生长因子是许多种细胞的丝裂原，其可促进血管的生成及成骨细胞基因的表达。

With respect to cellular involvements, broblasts are the most well-studied cell population.

相关细胞，成纤维细胞是被深入研究的细胞群。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐