成纤维细胞

MSC induced by 5-aza could be identified by the positive staining for Troponin I and Connexin43. The number of cells stained positively in the 10umol L-1 and 20umol L-1 5-aza was visually greater than that of other groups , but more than 50% the cells necrosed in 20umolL -1 5-aza. Electron microscopy revealed sarcomeric organization and gap junction.

结果MSCs体外贴壁生长，呈成纤维细胞样形态，经5一aza诱导后细胞体积增大，变为球状或者长杆状，4w后荧光免疫细胞化学方法检测肌钙蛋白、肌动蛋白及Connexin43表达阳性，10umol·L一'5一aza组及20umoz·L一'5一aza组阳性表达较高，但20umol·L一'5一aza组约50%细胞死亡。

In a word, mad1 gene or mad1 combining with c-myc gene could inhibit TCCB cell proliferation but not normal fibrocytes in vitro and in vivo, which targeted hTERT, suppressed its transcription and expression, and down-regulated the expression of cyclin D1 and TGF-β1, to further block the cell cycle, change cell morphology and suppress proliferation.

综上所述，mad1基因或mad1联合c-myc基因能在体内外抑制TCCB细胞的增殖，但对正常成纤维细胞无明显影响，二者以hTERT为作用靶点，抑制其转录和表达，并下调cyclin D1和TGF-β1的表达，进而阻止细胞周期、改变细胞形态和抑制癌细胞的增殖。

METHODS By means of cell culture and flow cy-tometry we studied the effect of fluid nitrogen cryopreser-vation on the expression of MHCⅠantigen of smooth mus-cle cells and fibroblasts of aortic homografts of rats.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

METHODS By means of cell culture and flow cytometry we studied the effect of fluid nitrogen cryopreservation on the expres sion of MHC Ⅰ antigen of smooth muscle cells and fibroblasts of aortic homografts of ra ts.

采用细胞培养技术和流式细胞仪技术研究液氮低温保存对大鼠主动脉平滑肌细胞和成纤维细胞主要组织相容性抗原Ⅰ类表达的影响。

Basic fibroblast growth factor ; lymphokine activated killer cells ; cell division ; interleukin 2; bladder neoplasms ; immunologic cytotoxicity

碱性成纤维细胞生长因子；淋巴因子激活的杀伤细胞；细胞分裂；白细胞介素-2；膀胱癌；免疫细胞毒性

RESULTS: Positive immunohistochemical signals of MMPs and TIMPs could be found in fetal skin at different gestational periods.

结果 MMP -2、MMP -3、MMP -9、TIMP -1和TIMP -2在不同时期的胎儿皮肤内均有表达，它们主要分布于表皮细胞、成纤维细胞、毛囊和汗腺上皮细胞以及血管内皮细胞的胞浆中。

Mouse fetal tissues could be cut into uniform small pieces by scissors cutting and needle grinding, which grew well in tissue culture and great amounts of cells grew from the attached small tissue clump to form primary mouse embryonic fibroblasts monolayer.

将这些组织块培养48h，大量的梭形细胞从贴壁小组织块的边缘游离出的，细胞密度大，细胞生长旺盛，很快形成原代小鼠胎儿成纤维细胞单层。

Pue group is more formation of cartilage cells,cartilage cells,necrotic degeneration of the surface than the model group and the Sodium Chloride group with light rare exudation of inflammatory cells by microscope,we could see new blood vessels and fibroblasts.

光镜观察见葛根素组软骨细胞较平整，表层软骨细胞坏死变性较模型组和生理盐水组轻，炎性细胞渗出物少见，可见新生血管和成纤维细胞增生。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中，待细胞生长融合后在培养板上划线，造成无细胞的裸露区，分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液，每6h观察记录移行至裸露区的细胞。

In the present study, CHL cells were cultured routinely in MEM medium for 3 days. Then the cells were treated with sham exposure , 0.4mT 50 Hz sinusoidal MF , 0.4mT noise MF and the combined noise and 50Hz MF for 3 min and 15min, respectively. After exposure, the cells were lysed, and the proteins were extracted.

实验中，将中国仓鼠肺成纤维细胞分别经0.4mT工频磁场、等强度的噪声磁场、工频磁场与噪声磁场的复合磁场辐照及假辐照处理3min和15min后，裂解细胞，提取细胞蛋白质，以Western印迹技术，测定并比较细胞内SAPK磷酸化的变化。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐