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成纤维细胞

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It has several kinds of functions : It could accelerate blood coagulation; has chemotaxis to monocyte and neutrophil , could appeal to fibroblast and cartilage cell to move to the region of the injury, takes part in the signal transduction and activation in cell , and takes part in the growth and differentiation of cell and repair in trauma.

它的功能多样,能加速血液凝固,对单核细胞和中性粒细胞具有化学趋化作用,能吸引成纤维细胞和软骨细胞向损伤区域移动,参与细胞的信号传导与活化,细胞的生长及分化,创伤修复等一系列重要生理和病理过程。

Proliferation of fibroblastic cells in bone marrow,caus in g anemia and sometimes enlargement of the spleen and liver.

骨髓纤维化,骨髓纤维变性在骨髓中成纤维细胞的扩散,常引起贫血症,而且有时也会导致脾和肝的增大

Result The flexibility of the scaffold material was powerful. The load-elongation curve of the mechanical testing was similar to that of ACL, the maximum load, the ultimate stress and the Young's modulus of the scaffold materials were 52.61 N, 14.96 MPa and 202.08 MPa respectively. Human ACL cells displayed the representative characteristercs of the ligamentous fibroblasts, and synthesized extracellular matrix, such as type Ⅰand Ⅲ collagen protein. The scaffold materials had no cytotoxicity. Human ACL cells adhered, grew and proliferated well both on the surface and in the holes of the scaffold materials.

结果]韧带支架材料的柔韧性强,拉力测试的负荷-拉伸曲线与韧带的拉伸曲线相似,其最大负荷、极限应力和弹性模量分别为52.61N、14.96MPa和202.08MPa;体外分离培养的人前交叉韧带细胞呈典型的成纤维细胞特征,能在体外分泌Ⅰ、Ⅲ型胶原等细胞外基质;支架材料无细胞毒性,人前交叉韧带细胞可在支架材料上黏附、生长并分泌细胞外基质。

Fig 4 Few of rough endoplasmic reticula, mitochondrid and co llagen can be seen in the cytoplasm of fibroblast, the ribosome of rER is degran ulated, the nucleous is abnormal, there are a lot of granule (maybe the antisens e oligodeoxynucleotide) in the cytoplasm TEM×5200

图4反义核酸组的瘢痕成纤维细胞粗面内质网较少、有脱颗粒现象,线粒体少,胞核畸形,胞内有吞噬颗粒,细胞周围胶原纤维较少TEM×5200

In order to study the mechanism of the inhibitory effect of salvia miltiorrhiza and tetramethyl pyrazine on scar fibroblast, the DNA content of fibroblast and the all distribution in cellular cycle was measured by FCM. The hypertrophic scar tissue of chest was chosen for primary culture of fibroblast.

摘 要 应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化,发现药物在一定浓度作用下,DNA指数无明显变化;丹参使C2-M期细胞分布增多,G2-M期延长;川芎嗪使G2-M期细胞分布增多,G2-M期、S期延长;药物使细胞群体倍增时间延长,与浓度呈明显直线正相关。

Results Most OECs were bipolar or tripolar, and the purity was estimated to be over 88.7%. The non-OECs cells consists of astrocytes, neurons, oligodendrocytes and fibroblas.

结果 体外培养的新生小鼠嗅鞘细胞主要为双极或三极细胞,其纯度可以达到88.7%以上,污染细胞中有星形胶质细胞、神经元、少突胶质细胞和成纤维细胞

The conclusions were that the inhibitory effect of SM was the result of inhibiting the mitosis of cells and the cellular cycle be at a standstill in G2-M stage.

应用流式细胞光度分析法测定丹参和川芎嗪对体外培养的瘢痕成纤维细胞DNA相对含量和细胞周期时相变化,发现药物在一定浓度作用下,DNA指数无明显变化;丹参使C2-M期细胞分布增多,C2-M期延长;川芎嗪使C2-M期细胞分布增多,C2-M期、S期延长;药物使细胞群体倍增时间延长,与浓度呈明显直线正相关。

Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养;测定细胞计数,细胞活性(WST-1分解)和DNA合成,并以流式细胞仪测定细胞周期等以表示细胞增殖。

Methods: Cardiac myofibroblasts derived from heart of New Zealand rabbit were cultured and identified. Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养;测定细胞计数,细胞活性(WST-1分解)和DNA合成,并以流式细胞仪测定细胞周期等以表示细胞增殖。

METHODS: We assessed the number of fibrocytes in bronchial biopsy specimens and peripheral blood from subjects with mild-to-severe refractory asthma versus healthy control subjects.

我们评估了轻-重度难治性哮喘患者气道活检标本和外周血中成纤维细胞的数量,同时通过体外实验,探讨了调控纤维细胞向气道平滑肌移行的可能机制。

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