成纤维细胞

Methods HPDLFs were primary cultured from tissue explants, and the cells of the 5th to 8th passages were used after immunohistochemical identification of keratin and vimentin expressions. The cells were divided into 5 groups and treated with TP at 1, 0.5, 0.25, 0.125, and 0.0625 mg/ml, respectively, with another group without TP treatment as the blank control group. Cell counting and MTT colorimetric assay were performed to assess the cell proliferation, and flow cytometry was employed to determine the DNA content of the HPDLFs.

采用组织块培养法培养原代人牙周膜成纤维细胞并传代，经免疫组化SABC法检测角蛋白和波形丝蛋白鉴定，取5~8代细胞用于实验；按茶多酚不同浓度分1mg/ml、0.5mg/ml、0.25mg/ml、0.125mg/ml、0.0625mg/ml组和空白对照组，采用细胞计数法、MTT法检测细胞增殖情况，流式细胞术检测细胞DNA含量。

It's also where cells called fibroblasts produce collagen and elastin fibers.

它的细胞，也是所谓的成纤维细胞产生胶原蛋白和弹性蛋白纤维。

Tumor cells grew actively in the tumor tissues of the control group. Prodrug therapy group: small amount of tumor cells were denatured vacuously,others were infiltrated by lympholeukocyte,and the growth of tumor cells were surspressed.Prodrug themochemotherapy group: what we can see that tumor cells were denatured vacuously, mesoplasts crinkled, cellular boundary disappered, only a small number of tumor cells remained, fibroblast were seen scatteredly, tumor cells were invaded by a lot of lympholeukocyte and eosinophilic granulocyte. Normal liver tissues, stomach tissues, lung tissues, pancreas tissues, small intestine tissues, large intestine tissues showed normal shape.

对照组肿瘤组织见肿瘤细胞生长活跃；前药治疗组肿瘤组织见有少量细胞空泡变性，少量淋巴细胞浸润，肿瘤细胞生长受到抑制；前药热疗组肿瘤组织可见肿瘤细胞空泡变性，细胞核皱缩，边集甚至消失，仅残留少量肿瘤细胞，并可见散在的成纤维细胞，大量淋巴细胞和嗜酸性粒细胞浸润；3组裸鼠正常肝组织、胃、肺、胰腺、小肠、大肠组织均呈正常形态学，无病理性损伤改变。

Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology.

背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组，给药组涂抹红鹿制剂，对照组涂抹化妆品基质，连续涂抹60 d；应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。

Results:Wound healing rate in group A was higher than that in group B and the wound healing time was 1～3 days earlier in group A.More active proliferation of epidermic cells around wounds showed in group A.The function of fibroblasts in wounds,collagen synthesis and secretion,were more vigorous in group A than those in group B.

结果：A组创面愈合率高于B组，其创面愈合时间较B组提前1～3 d；创周表皮细胞增殖也较B组活跃，创面成纤维细胞较B组呈现出更旺盛的合成与分泌胶原纤维的功能。

We hypothesized that MCP-1 may mediate fibrotic remodeling through recruitment of mononuclear cells and direct effects on fibroblasts.

我们假设MCP-1 可能通过单核细胞的复原和成纤维细胞的影响造成间接的纤维化重构。

After 21 days, the fibroblasts were less, capillary was hyperemic and expansive, with the collagen began to reconstruct between the acupotomology intervention group and the blockade control group.

造模后21 d针刀干预组与封闭对照组大鼠组织内成纤维细胞较少，毛细血管增生扩张，胶原重建开始；模型对照组成纤维细胞较多，胶原分泌增多。

HE staining revealed that collagen fiber, fibroblasts and granulation tissue and synechia belt around tendons and stomas were apparently less in contrast to control group.

术后4周苏木精－伊红染色结果表明，实验组腱吻合口被胶原纤维连接，腱周及吻合口周围胶原纤维、成纤维细胞及肉芽组织明显较对照组少，腱周纤维组织较对照组粘连轻，粘连带稀少。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用，按美国ASTM标准制备NiTi、317L合金的金属浸提液；&组织块培养法&原代培养食管壁疤痕的成纤维细胞，传代后以金属浸提液进行培养，分组后分别培养4、24、48、72 h，MTT法检测不同培养时间后Fb增殖功能的变化；按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验，即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间，术后2、12周取出包埋组织，分析试件周围组织的病理变化，并进行胶原纤维染色，观察纤维形成状况。

Since an excessive cell growth is considered as the main pathological event, cell proliferation model occupy most of the animal models, and these cells include retinal pigment epithelial cell, fibroblast cell, cartilage cell and vascular endothelial cell.

由于PVR的主要病理变化是细胞的过度增生，因而动物模型多以细胞增生模型为主，细胞种类有视网膜色素上皮细胞、成纤维细胞、软骨细胞、血管内皮细胞等。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。