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成纤维细胞

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Recently, a newly identified cell type, the fibrocyte, has been reported to contribute to wound healing and fibrotic conditions such as hypertrophic scarring.

近年来,循环成纤维细胞作为一种新型细胞被发现并鉴定,有报道表明这种细胞对创伤愈合和类似瘢痕肥大的纤维变性具有促进作用。

Aminoglycosides suppress termination at nonsense codons and result in functional improvement in a cystic fibrosis bronchial epithelial cell line, in muscle cells from mdx mice and in fibroblasts from individuals with Hurler syndrome.

氨基糖苷类物质可以通过抑制无意义密码子的终止作用,改善囊性纤维化的支气管上皮细胞、患肌营养不良的肌细胞和Hurler综合征病人的成纤维细胞的功能。

Methods Epithelial cells and fibroblast from ameloblastoma, keratocyst, dentigerous cyst, radicular cyst and oral mucosa were cultured and observed by phase-contrast microscope.

原代体外培养成釉细胞瘤细胞、牙源性角化囊肿、根端囊肿及口腔粘膜的上皮细胞和成纤维细胞,倒置光相差显微镜观察。

RESULTS:①The cells showed fusiform shape, reticulate at low density and fasciculate at high density under inverted phase contrast microscope. Mouse anti-Vimentin was positive.

结果:①巩膜成纤维细胞鉴定:倒置相差显微镜下观察,细胞透亮呈梭形,在细胞密度较低时细胞排列成网状,密度较高时呈束状;波形蛋白染色阳性。

Results ⑴ The cell proliferation was inhibited by TET. It showed thatdivision index and cell number during G0-G1 were decreased, while S and G2-M phases were lengthened with dosage and time dependency in dosages of TET from 2mg/L to 8mg/L; besides, oval-shap cells, karyopyknosis and fragmentation were observed under light microscope. The inhibitory effect in T2 group was statistically similar to that in triamcinolone.

结果⑴ TET在2mg/L~8mg/L之间呈剂量及时间依赖性地抑制瘢痕成纤维细胞的增殖,使生长曲线不同程度的压低、分裂指数降低、G0-G1期细胞数减少、G2-M期增多、S期与G2-M期延长,光镜下见细胞突起变短,细胞由梭形变成椭圆形,核固缩、碎裂。P.C的改变近似于T2组(4mg/L TET)。

In addition, we have found other cellular sources like periportal fibroblasts or bone derived fibrocells.

另外,我们发现还有其他细胞来源例如门静脉周围成纤维细胞或者骨源性纤维细胞。

The first passage cells were initially pured by enzyme digestion, then pured by enzyme digestion and repeated strapping alternatively in the second course of transferation and inoculation, the third period cultured cells showed typical clonali growth and were long fusiform fibroid cells with short growth cycle.

首次传代时应用胰酶消化法进行成纤维细胞的初次纯化,第2次转种时先后应用酶消化法和反复贴壁法再次进行细胞纯化,第3代以后基本可获得呈典型克隆性生长、长梭形纤维样细胞,生长周期短。

P<0.01. 28d, q=4. 0452, p<0.05 . Corresponding pathologic result shows that the corneal stromal of height edema increase thickness to have infiltration of inflammation cells with hyperplasia of fibroblast cell and large vessel. The collagen fiber in stromatic layer is dissolved and destroyed seriously, suggesting that in addition to HSV-1's direct destructuring effect, inflammatory cells that might play an important role in the pathology of HSK induce the immune-pathological reaction.

相应的组织病理学研究结果显示(UV-B 14天):病毒对照组角膜基质层高度水肿、增厚,并有大量中性粒细胞等炎性细胞浸润、成纤维细胞和新生血管增生,胶原纤维肿胀、分解等,说明除HSV-1对角膜组织直接破坏外,炎性细胞介导的免疫病理反应在复发性HSK发病机理是重要的因素。

In this study, we observed the effect of mast cell tryptase on cultured human renal interstitial fibroblasts, so as to further investigate the effect of tryptase on renal fibrosis.

本实验研究肥大细胞类胰蛋白酶及蛋白水解酶抑制剂苯甲脒(benzamidine hydrochloride hydrate,BZ)对人肾成纤维细胞的影响,以探讨肥大细胞类胰蛋白酶在肾纤维化过程中的作用。

Immunoelectron microscopy showed that the distinct ultrastructure of fibroblast and the group, spot gold particle specificity distribution shape. The positive markers of CTGF were mainly located in the RER,while there are expression in RER ribosome, Cf, ECM, desmosome connection, euchromatin,et al.

免疫电镜标记显示成纤维细胞细胞超微结构清晰,金颗粒呈团状、点灶状分布,特异性强,CTGF蛋白阳性标记主要位于粗面内质网,粗面内质网核糖体、胶原纤维、细胞外基质、桥粒连接、常染色质等部位也有表达。

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