成神经细胞

Objective To study the influence of intrauterine hypoxia to offspring rat Ethology change and nerve cells in brain hippocampus degeneration and apoptosis.

目的研究宫内缺氧(intrauterine hypoxia ，IH)对子代成鼠行为学改变及神经细胞变性、调亡的影响。

Therefore, the study of Hes1 expression in neural stem cells and its relationship with adult neurogenesis is put on the agenda.

因此研究Hes1在成体神经干细胞中的表达及其与成体神经细胞生成的关系也就被提上日程。

RESULTS: Otocyst cells differentiated and proliferated and showed several morphotypes, mainly including epitheliallike cells, fibroblastlike and neuronlike cells.

结果：培养的细胞在体外进行分化和增殖，细胞形态主要有上皮细胞形态、成纤维细胞形态和神经细胞形态3种。

RESULTS摘要: Otocyst cells differentiated and proliferated and showed several morphotypes, mainly including epitheliallike cells, fibroblastlike and neuronlike cells.

结果摘要：培养的细胞在体外进行分化和增殖，细胞形态主要有上皮细胞形态、成纤维细胞形态和神经细胞形态3种。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

Dr Yamanaka's team has, for instance, been able to persuade its stem cells to turn into nerve cells and heart cells.

比如，Yamanaka博士的团队已经可以让这些制造出来的干细胞转换成神经细胞，心脏细胞。

The outer neuroblastic layer before it divided to inner and outer nuclear layer and outer complexiform layer and gonglion cell layer showed a temporal PCNA positive which was related to proliferation. The three cell layers had another PCNA positive when finished time changed from outer to inner after their morphologic development basically completed( outer nuclear layer:11～15 days,inner nuclear layer: 8～16 days, gonglion cell layer:16～21 days).

PCNA显示在外成神经细胞层分化为内、外核层和外丛状层之前，和节细胞各有一次和增殖相关的阳性表达。3个细胞层在形态学的分化基本完成后，又出现了一次结束时间是由外向内变化的阳性过程（外核层：第11天至15天：内核层：第8天至16天，神经节细胞层： 16天到21天，这一次阳性表达过程可见到细胞形态向成年形态的转化。

RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

结果：用诱导分化液作用后，未见小的、伴有突起的放射状形态的神经细胞；抗β Tubulin Ⅲ对经神经元胶质细胞诱导分化液作用后的人成肌细胞、增殖培养液培养后的各代人成肌细胞及仅含20 mL/L HS分化液分化形成的肌管细胞染色均为阳性； C2C12细胞β Tubulin Ⅲ抗体染色阴性；上述所有细胞抗Neurofilament Mr 68×103和抗GFAP染色均为阴性。

Electronic microscopic observations showed that the swollen mitochondria with cristae blurred appeared in some pyramidal cells. Some pyramidal cells showed degenerative changes with some disappeared endoplastic reticula and ribose nuclear protein and increased lysosomes. The amount of extends endothelial projections going to the lumen of capillaries were increased.

同时伴有超微结构的改变，如神经细胞线粒体肿胀，内质网扩张，染色质凝集成块状，毛细血管外间隙扩大，髓鞘变性坏死，微管排列紊乱，小胶质细胞有吞噬变性坏死的神经纤维成分等。

MyosinⅩ, a vertebrate-specific member of myosin super-family, can be detected in most tissues and cells, such as kidney, liver, brain, and so on. Full-length MyosinⅩcan stimulate the formation or stabilization of filopodia. As an actin-based motor protein, MyosinⅩplays a key role connecting cell membrane proteins and cytoskeleton.

肌球蛋白Ⅹ是脊椎动物所特有的细胞骨架蛋白，在脑、肝、肾等大多数组织中都有表达，它的显著特点是定位于细胞丝足的顶端和片状伪足的边缘，MyosinⅩ作为一个动力蛋白分子，起到连接细胞膜蛋白与细胞内骨架蛋白的桥梁作用，因此推测它可能在SVZa成神经细胞的迁移过程中起到重要作用。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。