成熟卵泡

17 To 12 * 12 * 11 The result is, most great to see a doctor to eat Pills Queensland, on BC-month follicle is a very good round, but did not succeed failed to grasp the opportunity this month to eat do not know the drugs are of no use, there is no chance of immature follicles, the largest non-up may be due to washing foot massage, doctors said he would let the弄散follicle, it is no wonder there are many follicles but not dominant follicle, what I eat now Pills follicle-kun will you grow up, week 2 to BC, I hope it will be great together.

5月4号的月经，15号去BC卵泡是12*10，17号去结果是12*11，大都大不起来，去看了医生开了昆灵丸吃，上个月BC卵泡是很好很圆的，但没有把握机会没能成功，这个月不知道吃了这个药有没有用，卵泡不成熟就没机会了，这次大不起来的原因可能是去洗脚，按摩，医生说会吧卵泡弄散了，难怪卵泡有很多个但没优势卵泡，请问现在我吃坤灵丸卵泡还会长大吗，星期2去BC，希望会大起来吧。

The rates of apoptotic granulosa cells in all kinds of follicles in immature gilts were ashigh as atretic follicles;it decreased significantly after injection of PMSG,but it was higherand lasted shorter than that of mature pig.

性未成熟仔母猪卵巢中各类卵泡颗粒细胞凋亡比例很高，接近闭锁卵泡的凋亡比例；注射PMSG后则各类卵泡颗粒细胞凋亡比例显著下降，但高于性成熟母猪注射PMSG后的凋亡比例，并且维持的时间也较短。

Generally, most of the follicles undergo atresia during their development in mammals, and only a few grow to maturation and ovulation. For many years, the mechanisms of follicular atresia have been one of the foci of study. Recently, with the development in studies on cell apoptosis, it has been proved that follicular atresia is a process of cell apoptosis. However, the endocrinological and molecular evens underlying follicular development and atresia and granulosa cell apoptosis in the goat has not been studied.

中文题名山羊卵泡发育、闭锁及其调控机理的研究副题名外文题名 Studies on follicular development and atresia and their regulatory mechanism in goat 论文作者于元松导师谭景和教授学科专业基础兽医学研究领域\研究方向动物胚胎学与胚胎生物工程学位级别博士学位授予单位东北农业大学学位授予日期2002 论文页码总数71页关键词山羊卵泡发育馆藏号BSLW /2003 /S827 /3 哺乳动物卵泡在发育过程中绝大多数闭锁退化，只有少数卵泡发育成熟至排卵。

Finally, the experimental animal symptom distinct improvement, the ovary obvious increase, the ovary obviously primitive graafian follicle and the massive primaries follicle, the secondary follicle and approach the mature graafian follicle, Huang Tijiao many, nature few, the nature gland is rare.

结果，实验动物症状明显改善，卵巢湿重明显增加，卵巢可见原始卵泡及大量初级卵泡、次级卵泡和接近成熟的卵泡，黄体较多，间质少，间质腺少见。

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF。

Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平对卵丘扩展有影响，但随培养时间的延长，对激素的要求有逐步减少的趋势；猪卵丘扩展不依赖于卵母细胞，卵母细胞核成熟也与卵丘扩展无关；GV期卵母细胞、小腔卵泡内颗粒细胞和成熟卵的卵丘细胞都有较高的分泌CEEF能力；3-6mm卵泡的壁颗粒细胞不能分泌CEEF，但3-6mm卵泡的卵泡液中有CEEF。

With the great progress in embryo engineering during the past decade, the maturing oocytes with high qualities and quantities are increasedly required.

然而由于目前对卵泡发生和发育的调节机制仍知之甚少，因此大大制约了利用卵泡体外培养和体外成熟技术生产成熟卵母细胞的成功率。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后，分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h，研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

(1)FM with an incidence of 27.0% might be an important cause of infertility.(2) FM results finally in follicular atresia or multiple immature luteinized follicles.

(1)FM是不孕的一个重要原因；(2)FM结局有二，一为卵泡闭锁，另一为未成熟卵泡黄素化。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母细胞时，将同周期成熟卵丘复合体切出部分卵丘细胞，用1 mL注射器抽打分散细胞，贴壁培养。113个治疗周期中，298枚生发泡期卵母细胞经3种不同培养液体外成熟培养(同一病人的生发泡期卵被随机分到某同一组中)：第1组28个周期中73枚：基础培养液+卵泡液；第2组40个周期中115枚：A液+分散贴壁的卵丘细胞；第3组45个周期中110枚：A液+分散贴壁的卵丘细胞+促卵泡生成激素+表皮生长因子。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。