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The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P<0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10%FBS,细胞浓度为100个/400μl,发育培养体系为NCSU-23+BSA;用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v/20μs/2次+600μMγ-BLI;以活力为0.1左右的冷冻精液进行IVF时,精子浓度为10~7,共孵时间为6h,并在成熟处理时只部分去除卵丘细胞;MⅡ卵母细胞冷冻时,方法为移液枪头法+离心法,冷冻后激活的2-细胞卵裂率比IVF高。

Effects of EGF on the in vitro maturation and parthenogenetic development of bovine oocytes were studied.

在卵母细胞体外成熟液中分别添加10,30,50ng/ml表皮生长因子,24h检查成熟率,并进行孤雌激活。

Adolencence is the transmit peried for individuals from maturity to maturity .whether on psycolog , phys or mind, adolencence has different characteristics,the youngsters consumption depends upon the inherited phycological factor.compared to other group consumers,theypwns their owen clear psychological characterictics.the youth is active in mind,enthusiastic and open ,imaginative , easy to accept new things , apt to seek cuiosityes.

青年期是个体从不成熟走向成熟的过渡时期。无论在心理还是在生理及思想上都有其不同的特点。青年人的消费,受其内在的心理因素支配,同其他消费群体相比,具有鲜明的心理特征。青年人思维活跃,热情奔放,富于幻想,容易接受新事物,喜欢猎奇,反映在消费心理和消费行为方面,表现为追求新颖与时尚,追求美的享受,喜欢代表潮流和富于时代精神的商品。

The effect in the shoot is caused by the increased influx, mediated by HKT1;1, of Na+ into stelar root cells, which is demonstrated in planta and leads to a reduction of root-to-shoot transfer of Na+.

本研究介绍了拟南芥成熟根部Na+运输体HKT1;1的表达降低了37%到60%Na+在芽部的累积量,HKT1;1在成熟根部专一性的表达是通过增强子诱导表达系统过量表达来实现的。

In oocytes matured in vitro for 48h, polyspermy ratio is 73%, and lower than that in 36h(83%)and 24h(80%).

结果显示,体外成熟48h的卵母细胞其多精受精率为73%,比体外成熟36h(83%)、24h(80%)的卵母细胞要低。

In oocytes matured in vitro for 48h, polyspermy ratio is 73%, and lower than that in 36h(83%)and 24h(80%).

结果显示,体外成熟48h的卵母检测细胞其多精受精率为73%,比体外成熟36h(83%)、24h(80%)的卵母检测细胞要低。

Results showed that contents of IAA and ABA in peduncle, bourse and the tissues around abscission zone among several cultivars were very different, but their trend of change was similar, IAA content decreased and ABA content increased toward harvest time for all the cultivars. Ethylene evolution in fruit of the cultivars with higher preharvest drop was evidently higher than the cultivars with less preharvest drop. Cellulase activity in the tissues around abscission zone of the cultixars with higher prehanest drop rapidly increased toward harvest time.

结果表明:不同品种果柄、果台和离层部位组织中IAA和ABA含量变化有差异,但它们变化的总趋势相似,都是随着果实成熟IAA含量下降,而ABA含量上升;不同品种的成熟果实中乙烯发生量有很大差异,以落果多的品种显著大于落果少的品种;采前落果重的品种离层部位组织中细胞壁分解酶的活性在果实成熟期急剧增加。

Functionally known genes were obtained after Blastx analysis, 237 of them were from the subtractive library of stage of proembryo mass and 279 from that of somatic embryo maturation.

成功构建了Y35体细胞胚原胚团阶段和成熟阶段的差异表达基因文库,获得了868个差异表达的UniESTs,其中原胚团阶段400个,体细胞胚成熟阶段468个。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后,分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h,研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

Objective To study the mechanism in which the personality, copying style influenced survival time by changing immunity function in the patients with gastric cancer Methods The Minnesota Multiphase Personality Inventory, coping style questionnaires and immunity function were measured in patients with gastric cancer on late stage, gastric cancer patients with longer survival time, and normal control Results Psychopathic deviate, social introversion subscale scores of MMPI in groupⅡ were significantly lower than that in the other two groups ( P 05) Statistical difference were found between groupⅠand groupⅡ in problem saving factor and help seeking factor of CSQ ( P 05) Natural killer cell level in groupⅡ was significantly higher than in groupⅠ( P 05) There was negative correlation between the NKC and depression, psychopathic deviate, paranoia, psychasthenia, schizophrenia subscale scores of MMPI, there was positive correlation between NKC and SP, coping type in patients with gastric cancer Conclusion The gastric cancer patients with longer survival time are more extroversive and have more mature coping styles Their NKC level is relatively higher The results may be advantageous to improve the prognosis of gastric cancer

作者中文名:王健;邹义壮;唐丽丽;李杰;张东;金山;刘娟摘要:目的研究胃癌患者的生存期与个性特征、应付方式等心理因素,以及免疫功能的关系。方法对胃癌晚期患者、胃癌较长生存期患者及正常健康人分别进行明尼苏达多相个性测验、应对方式问卷、免疫功能测试,并做相关分析。结果(1)MMPI:胃癌Ⅱ组人格障碍评分(42.0±10.5)及社会内向评分(42.2±11.6),低于其他两组[胃癌Ⅰ组为(49.3±11.2)分和(48.3±9.4)分,对照组为(48.6±10.9)分和(39.9±11.5)分;P均<0.05];(2)CSQ:解决问题和求助因子分,胃癌Ⅰ组[分别为(0.68±0.27)分和(0.42±0.18)分]均低于胃癌Ⅱ组[分别为(0.82±0.18)分和(0.65±0.21)分;P<0.05和P<0.01],胃癌Ⅱ组更多采取成熟的应对方式;(3)自然杀伤细胞:胃癌Ⅱ组水平(21.4±9.2)高于胃癌Ⅰ组[(14.2±8.8);P<0.05];而NK细胞水平与MMPI的抑郁(r=-0.35)、人格障碍(r=-0.39)、偏执(r=-0.29)、精神衰弱(r=-0.30)及精神分裂(r=-0.30)等量表呈负相关,与CSQ的解决问题因子(r=0.35)及应付方式类型(r=0.34)呈正相关。结论与胃癌Ⅰ组比较,胃癌Ⅱ组更为外向,负性情绪较少,更多采取成熟的应对方式,其NK细胞水平较高,对胃癌的预后产生了积极的影响。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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