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To date,it has been documented after transfusion of unirradiated blood components to at least 87 patients:in patients with severe combined immunodeficiency,thymic hypoplasia,and Wiskott Aldrich syndrome premature newborns and those with erythroblastosis fetalis; patients with hematologic malignancies including Hodgkin\'s and non-Hodgkin\'s lymphomas,acute myelocytic and lymphoblastic leukemias,chronic lymphocytic leukemia,and aplastic patients with solid tumors including neuroblastomas, glioblastoma,rhabdomyosarcoma,cervical carcinoma,small cell lung cancer,and germ cell tumor;patients after cardiac surgery and cholecystectomp-60;and in an apparently healthy 22-year-old woman.

目前为止,输注未辐照血液成分后,至少引起了87例TA-GVHD的发生,主要见于以下病人:严重的免疫缺陷病人、胸腺发育不全、Wiskott Aldrich综合症、早产儿、胎儿红细胞增多症、何杰金与非何杰金氏淋巴瘤等恶性血液病人、急性粒细胞性和淋巴细胞性白血病、慢性淋巴细胞性白血病、成神经细胞瘤等实体瘤病人、横纹肌赘瘤、宫劲癌、小细胞肺癌、微细胞瘤、心脏和胆囊手术病人。

Results: Among 38 transgenic mice surviving beyond one year.12(9 myc and 3 myc+ ras)mice developed multiple neoplasms.Most tumors were observed as lymphoblastoma and fibrosarcoma in histological types.

结果:在存活一年以上的38只转基因小鼠中有12只(9myc和3myc+ras)发生多种肿瘤,其组织类型以成淋巴细胞瘤和纤维肉瘤居多。

Context Pediatric acute lymphoblastic leukemia is the prototype for a drug-responsive malignancy. Although cure rates exceed 80%, considerable unexplained interindividual variability exists in treatment response.

根据文章的背景资料,儿科的急性成淋巴细胞性白血病的治愈率已经从1960年代的不到10%增加至如今的80%以上,但是这些病儿对治疗的反应却存在着相当大的且无法解释的个体差异。

Tumor cells grew actively in the tumor tissues of the control group. Prodrug therapy group: small amount of tumor cells were denatured vacuously,others were infiltrated by lympholeukocyte,and the growth of tumor cells were surspressed.Prodrug themochemotherapy group: what we can see that tumor cells were denatured vacuously, mesoplasts crinkled, cellular boundary disappered, only a small number of tumor cells remained, fibroblast were seen scatteredly, tumor cells were invaded by a lot of lympholeukocyte and eosinophilic granulocyte. Normal liver tissues, stomach tissues, lung tissues, pancreas tissues, small intestine tissues, large intestine tissues showed normal shape.

对照组肿瘤组织见肿瘤细胞生长活跃;前药治疗组肿瘤组织见有少量细胞空泡变性,少量淋巴细胞浸润,肿瘤细胞生长受到抑制;前药热疗组肿瘤组织可见肿瘤细胞空泡变性,细胞核皱缩,边集甚至消失,仅残留少量肿瘤细胞,并可见散在的成纤维细胞,大量淋巴细胞和嗜酸性粒细胞浸润;3组裸鼠正常肝组织、胃、肺、胰腺、小肠、大肠组织均呈正常形态学,无病理性损伤改变。

It contains clusters of lymphocytes and also filters the blood of old or worn-out cells.

它包含有成簇的淋巴细胞,筛除血液中衰老或破损的细胞。

The relationship of DNA ploidy level and SP-F with age,clinical stage, ascites, histologic type,pathological grade,lymphocyte infiltration and psammoma bodies was analyzed.

作者采用流式细胞技术,对24例新鲜速冻卵巢癌组织的细胞DNA含量及S期比例(S-phasefraction,SPF)进行了定量测定,分析了它们与患者年龄、临床分期、有无腹水、组织学分类、病理分级、肿瘤组织淋巴细胞浸润及砂粒体形成之间的关系。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后,利用SABC法检测CD80的表达;MTT法测定肿瘤细胞体外增殖能力;将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度;在同源淋巴细胞肿瘤细胞混合培养后,通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18,mChIL-18)的cDNA基因序列,设计一对特异性引物,应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因,对扩增片段进行T-A克隆,经PCR、酶切鉴定及测序验证,成功获得ChIL-18成熟蛋白完整基因的克隆。

The results display that the thymus-index, spleen-nidex, immune globulins of IgA, IgG, IgM and alexins of C3, C4 in the experiment groups were significantly higher than those of the control group (P.05); the spleen's lymphocytes translation stimulate indexes in the experiment groups were higher than that of the control group at a significance level(P.01). The peritoneal macrophages can enhance the ability of swallowing. The ability of licking up neutral red was depended upon the dosage of drug.

与对照组相比,试验组的胸腺指数、脾脏指数、免疫球蛋白和补体C3、C4都升高且显著水平为差异显著(P.05);脾脏淋巴细胞转移刺激指数均极显著地高于对照组(P.01), 75μg/mL组的脾脏淋巴细胞转移刺激指数达最高;腹腔巨噬细胞具有一定的吞噬能力,吞噬中性红能力与剂量成依赖关系。

Jude Children's Research Hospital in Memphis, Tenn., for acute lymphoblastic leukemia.

Jude 小孩研究医院里2169名接受治疗的患急性成淋巴细胞白血病的儿童和青少年。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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