成浆

Results It was demonstrated that β-TrCP expressed in oral epithelium, tooth bud, mesenchymal cell cytoplasm of ameloblast and edontoblast of different stage of tooth development.

结果β-TrCP在胚胎10.5、13.5、14.5、16.5、18.5d的小鼠牙胚上皮层与间充质层，以及出生后0、3、6d的小鼠成釉细胞与成牙本质细胞胞浆呈特征性表达。

"In making chocolate, the kernels of fermented and roasted cacao bean s are ground into a paste called chocolate liquor, which may be hardened in molds to form baking chocolate, pressed to reduce the cocoa butter content and then pulverized to make cocoa powder, or mixed with sugar and additional cocoa butter to make sweet chocolate."

巧克力制做工序是将可可豆的种仁发酵和焙烤，再磨成糊状，称巧克力浆，然后在模型内凝固成苦巧克力；加压除去所含的可可脂，随后磨成可可粉；或加糖及可可脂即可制成食用巧克力。

The effect of hemi-cellulose content on fiber horrification and paper performance in recycling is studied concerning soda-AQ reed pulp, SP reed pulp and KP soft wood pulp.

研究了烧碱蒽醌法苇浆、碱性亚钠法苇浆、KP法针叶木浆在回用过程中纤维保水值、纤维聚戊糖含量以及成纸性能的变化，结果表明纸浆在回用中打浆前后聚戊糖含量稍有下降，而保水值增加；随着回用次数的增加，聚戊糖含量变化不大，但保水值却大幅度下降；成纸性能在回用中逐渐下降，而光学性能则向良好的方向发展。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The protein levels of periostin in KFb (91.815±0.961) and HFb (70.166±2.250) were both higher than that in SFb (41.011±1.576, P.01). Conclusion: The expressions of periostin were increased in the fibroblasts of hyperplasic scars, which could be inhibited by hycirocortisone.

Periostin蛋白主要分布在成纤维细胞核周围的胞浆中，它在瘢痕疙瘩和增生性瘢痕的成纤维细胞中的表达（分别为91.815±0.961和70.166±2.250）高于在正常皮肤成纤维细胞中的表达(41.011±1.576， P.01)，两者分别增加55%和42%。

The synthesis of dvysocket luvigel and ofmaize after into mud, luvigel itself is also novosorb prefilming, its specific requirements to the bottle, it basically the same high temperature baking after solid prefilming cannot be yellow, luvigel do not in themselves, usually with yellow paint direct printing the appearance in yellow and kerosen luvigel of heavy are unavailable.

合成增稠剂成糊后与粘合剂配浆，增稠剂本身是高分子材料也会成膜，其具体要求基本上同粘合剂，其高温焙固成膜之后不能泛黄，增稠剂本身不能带有黄色，一般涂料直接印花中所用的外观呈黄色且煤油味重的增稠剂均不可用。

Sulphate pulp: Alkaline pulp made from wood chip s cooked under pressure in a solution of caustic soda and sodium sulphide.

重硫酸盐浆：把木片在苛性钠和硫酸钠溶液内加压蒸煮而成的硷性浆。

Soda pulp: Pulp produce from hardwood chip s cooked in caustic soda.

苏打浆：把硬木片放入苛性钠内蒸煮成的浆。

Soda pulp: Pulp produce from hardwood chips cooked in caustic soda.

苏打浆：把硬木片放入苛性钠内蒸煮成的浆。

In the intestinal and live homogenates,the half-lives of 5\'-L-valyl-ara-C were 46 and 34 min,respectively.

在大鼠胃液、小肠液和胃液中，5\'-L-valyl-ara-C的半衰期分别是28.4 h、7.5 h和84 min.5，-L-valyl-ara-C在小肠匀浆和肝匀浆中的半衰期很短，预示该前药口服吸收后，首过效应应该是其转化成母药的主要方式。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。