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After washed, the chromogen substrate was added and incubated for 30 minutes in the dark. Rinsed with distilled water, purple spots were observed and counted using a dissection microscope. The number of spots means the level of cytokine producted by sCTL. Analysis of ability of specific cytotoxic lymphocytes to lyse target cells: CytoTox96 Non-Radioactive Cytotoxicity Assay kit was used.

5特异性CTL裂解靶细胞能力测定:采用CytoTox96 Non-RadioactiveCytotoxicity Assay试剂盒,将经HBV core18-27短肽、重组乙肝病毒核心抗原共刺激培养13日的PBMC与HLA-A2〓的靶细胞按一定比例混合,靶细胞被裂解后,释放的乳酸脱氢酶与底物反应显色,酶标仪测定吸光度,吸光度与裂解程度成正比,按照公式计算百分裂解率。

Using Maxwell equation, Stoke' s law and penetration wave and reflected wave basic theory, research compound materials particle size, proportion of mixture and preview length and others.

再取混合后的粉末,将三种不同重量比的混合粉末与环氧树脂依重量百分率浓度5 wt%、10 wt%、15 wt%和20 wt%混合均匀成四种浓度的试片。我们归纳发现复合材料在粒径变化、混合比例、试片长度等…。

"And she saw that, owing to the peculiar effect of this convex mirror, the scarlet letter was represented in exaggerated and gigantic proportions, so as to be greatly the most prominent feature of her appearance."

由于这一凸面镜的特殊功能,她看到红字的映象极为夸张,显得比例极大,成了她全身最显著的特征。

Hester looked, by way of humouring the child; and she saw that, owing to the peculiar effect of this convex mirror, the scarlet letter was represented in exaggerated and gigantic proportions, so as to be greatly the most prominent feature of her appearance.

海丝特出哄孩子高兴的愿望,往里隔了瞧;由于这一凸面镜的特殊功能,她看到红字的映象极为夸张,显得比例极大,成了她全身最显著的特征。

Hester looked, by way of humouring the child; she saw that, owing to the peculiar effect of this convex mirror, the scarlet letter was represented in exaggerated gigantic proportions, so as to be greatly the most prominent feature of her appearance.

海丝特出哄孩子高兴的愿望,往里隔了瞧;由于 www.ddd§tt.com 这一凸面镜的特殊功能,她看到红字的映象极为夸张,显得比例极大,成了她全身最显著的特征。

A cost-plus contract means a construction contract in which the construction price is ascertained on the basis of the costs stipulated in the contract or costs negotiated otherwise, plus a proportion of these costs or a fixed fee.

成本加成合同,是指以合同约定或其他方式议定的成本为基础,加上该成本的一定比例或定额费用确定工程价款的建造合同。

A dot chart is superimposed on a scale cross-section drawn through the mass; the number of dots which fall within the mass outline multiplied by the anomalous density is proportional to the gravity effect at the origin of the chart.

布点图叠加在由质量绘制而成的相似比例横剖面上;落在质量略图内的点的个数乘于异常密度正比于原图中的重力效应。

The size of the epicondyles in Chinese is significantly smaller than that of the Westerns. The ratio between anterior posterior width of the medial epicondyle and the width of the STEA is 0.84, and is 0.87 between anterior posterior width of the lateral epicondyle and the width of the STEA. The distance from the epicondyles to the joint line is helpful to locate the STEA. The reliability is poor to locate the STEA by touch or PCL.

国人股骨远端上髁解剖大小明显小于欧美国家人群,股骨远端内外上髁的前后径与STEA宽度成一定比例,STEA的骨性标志与膝关节后侧及远端关节线的距离可为定位STEA提供一定帮助,通过术中触摸或PCL定位STEA的可靠性差。

Little Pearl- who was as greatly pleased with the gleaming armour as she had been with the glittering frontispiece of the house- spent some time looking into the polished mirror of the breastplate.

瞧啊!。瞧啊!海丝特出哄孩子高兴的愿望,往里隔了瞧;由于这一凸面镜的特殊功能,她看到红字的映象极为夸张,显得比例极大,成了她全身最显著的特征。

Trade finally or next online finishing, the scale that shops on oriented line directly truly still is less than 3 into, Imagine an user that buys a mobile phone, after if pass,checking a data to obtain enough goods information, can be directed directly a reliable C2C platform, to the client character, before going up to buy with respect to join, mix this two paces in buying.

最后的交易还是在线下完成,真正直接导向线上购物的比例还不到三成,"设想一个购买手机的用户,如果通过查资料获得足够商品信息后,能直接被导向一个可靠的C2C平台,对顾客而言,就连接上了购买前和购买中这两步。"

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?