成核

Results The small regenerating fibers, central location of nucelus and basophilia endochylema were observed under HE stain both in the patients with DMD and PM. Anachromasis of antiNCAM monoclonal immunohistochemistry stain was found in the regenerating muscle fibers. Positive Ezrin expression was also detected in the regenerating fibers. However, this expression tapered gradually as the mature process of skeletal muscle. The expression of Ezrin was negative in the mature fibers.

结果 DMD、PM患者被检肌HE染色所见再生肌纤维直径较小、核位于中央、胞浆嗜碱性；NCAM染色再生肌纤维深染；再生肌纤维Ezrin呈阳性表达，伴随肌纤维成熟Ezrin表达逐渐减弱，成熟肌纤维无Ezrin表达；成肌细胞Ezrin呈阳性表达。

Could be observed on 10th day after pollination, archesporial cell on 20thday, megasporocyte on 24th day, the functional megaspore on 30th day; and 8-nucleate embryo sac on 41th day. And then double fertilization finish and early globular embryo emerg.

授粉10 d出现指状突起，20 d形成胚珠原基，24 d大孢子母细胞发生，30 d功能大孢子形成，41 d发育为成熟的八核胚囊，而后完成双受精发育成早期球形胚。

A malignant transformant line 3T3 (T24) was obtained by transfection of mouse fibroblast NIH3T3 cells with pMAMneo-T24-ras plasmid. 3T3 (T24) cells were cultured continuously in the presence of benzamide , an NAD site inhibitor of poly polymerase .

将12位密码子突变的活化ras癌基因全cDNA序列克隆到真核表达载体pSV2neo和pMAMneo上，获得了相应的重组质粒pSV2neo-T24-ras和pMAMneo-T24-ras，将pMAMneo-T24-ras质粒利用基因转移方法导入小鼠成纤维细胞NIH3T3中，活化的ras癌基因可使受体细胞发生恶性转化。

Grasp the electron delocolization theory; be capable of judging the relative stability of allylic and benzylic free radicals and carbocations, know the relative stability of dienes and other multiple unsaturated hydrocarbons; grasp the characteristics for the nucleophilic substitution reactions of allyl halides and benzyl halides, and the addition reactions of conjugate dienes.

掌握电子非定域化理论；能够分析判断烯丙基、苄基自由基和正离子的稳定性和二烯及多重不饱和碳氢化合物的稳定性；掌握烯丙基、苄基卤代物的亲核取代反应特性和共轭二烯的加成反应。

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

The reaction of carbodiimide 14 with various neucleophiles was investigated. The selectivity of cyclization of the carbodiimide 14 with NH_3, RNH_2, R_2NH and NH_2NH_2 were studied, some of the gualidene-type intermediates were isolated and the possible mechanism was suggested.

探讨了碳二亚胺14与各种亲核试剂如NH_3、RNH_2、R_2NH以及水合肼的成环反应的选择性，并分离得到了部分胍中间体，提出了反应可能的机理，对反应的选择性进行了合理的解释。

These excellent properties of 9E-BHVC all mentioned above imply that it would be a true two-photon nucleic acid fluorescent probe if it can specially recognize the DNA in complex cellulous environment.So this dissertation has investigated the ability of 9E-BHVC on the cell nucleus imaging detaily.

与BMVC相比，9E-BHVC表现出作为双光子荧光探针更好的特性，例如具有更大的活性吸收截面和更高的结合常数，且具有更好的水溶性。9E-BHVC这些优良的性质预示着该分子如果能够在复杂的细胞环境中特异性地识别DNA，那么它将是真正意义的双光子核成像探针。

Compared to BMVC,9E-BHVC has many better features needed by a two-photon probe,such as larger two-photon action cross sections,stronger affinity to DNA and better water-solubility.These excellent properties of 9E-BHVC all mentioned above imply that it would be a true two-photon nucleic acid fluorescent probe if it can specially recognize the DNA in complex cellulous environment.So this dissertation has investigated the ability of 9E-BHVC on the cell nucleus imaging detaily.

与BMVC相比，9E-BHVC表现出作为双光子荧光探针更好的特性，例如具有更大的活性吸收截面和更高的结合常数，且具有更好的水溶性。9E-BHVC这些优良的性质预示着该分子如果能够在复杂的细胞环境中特异性地识别DNA，那么它将是真正意义的双光子核成像探针。

This phenomenon warrants further study. 4. Phylogeny study on Croomia and Stemona The relationship of the genus and the taxonomic placement of Stemonaceae The phylogenetic tree of matK, trnL-F, atpB-rbcL and ITS sequences data indicated that it was natural to place Stemona and Croomia in one family.

黄精叶钩吻属和百部属的分子系统学研究百部科属间关系和科的系统地位叶绿体DNA(matK、trnL-F和atpB-rbcL)及核DNA序列构建的系统树显示Stemona和Croomia形成稳定的单系类群，表明将这两个属放在同一个科是自然的，不支持将Croomia独立成科。

From the temperature (612℃) slightly under its liquidus, the nucleation number was most in the melt, the primary grains were fine and global, the number of grains per area was more, and the optimum holding time was near 30min at 612℃. From the temperature (600℃) below its liquidus, the nucleation number was more, but some nucleuses congregated and grew dendritically.

其中 ，液相线铸造法〔4〕是我们开发的一种新的制浆方法，该法使金属熔体在液相线温度附近保温一段时间并形核，然后浇铸成具有细小球形组织的适合半固态成形的铸坯。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。