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METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上,剪开大鼠颅骨,显露位于颅腔前方的嗅球,取出2只嗅球,在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织,保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层,剪成1 mm3小块分离获取单细胞悬浮液,调整细胞密度至1×107 L-1,接种在用poly-l-lysine包被的培养瓶或培养板中,于37 ℃、体积分数为5%的CO2培养箱中培养,第3天用无血清DMEM/F12培养基换液培养。

The combined application of seismic refraction and wide angle reflection methods,prospection of vertical seismic reflec...

地震折射波法和宽角反射波法的联合应用,近年来发展的垂直反射波法的探测,地震层析成像技术,地震面波的三维速度结构,物理模拟和数字模拟技术与波形反演、联合反演的应用,深化了对岩石层本体的认识。

Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology.

背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组,给药组涂抹红鹿制剂,对照组涂抹化妆品基质,连续涂抹60 d;应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。

The relative cell groth rate of L929 cell represented the proliferation of cells on materials with respect to control over time. The result showed that cytotoxicity of thermo-sensitive cell culcure dish was none or little,which can be used for lab use.Additional cell culture was performed with two types of celland cell sheets detachment were observed by reducing the culture tempareture after the confluently growth of the two types of cells.

进一步利用两种不同的细胞永生化的成牙本质细胞系和人牙周膜细胞在接枝有PNIAPm的培养皿上进行培养,观察到细胞可以正常贴壁,生长并增殖,并且在形成连续的细胞层之后,通过降低温度,获得了完整的细胞层结构,结合前两部分实验结果,证实了利用等离子体预处理和紫外线照射接枝制备温敏性培养皿的可行性,为后续研究提供了实验基础。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现:小线角木蠹蛾性信息素分泌腺位于腹部末端8~9节之间,是一个由节间膜特化而成的上皮结构,为一可外翻的腹褶,腺体表面分布着饱满的锥形体,羽化后2天未交尾的雌蛾腺体细胞呈单层排列,腹面中央由密集的柱形上皮细胞组成,细胞排列向两侧延伸至背部,其形状由柱形逐渐变为扁平形,细胞核为椭圆形,细胞与细胞间有明显的胞连接,细胞基底膜基褶较多,质膜上分布着微绒毛,并与内表皮连接,内表皮上有多层几丁质,外角质层染色较深,细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

The author gives the hardware structure of the tomographic imaging system and analyzes its characteristics in details.

论文分析了电容层析成象系统的硬件结构,结合我们建立的10电极电容成象装置,详细分析了电容测量电路的特性以及整个传感器电路在设计、制作中的一些特殊措施。

The liquating of crystal is useful to its shape and size and a thin layer of glass will bring about which is helpful to glaze gloss. Through cross experiment design, the reasonable range of oil-drop glaze chemical composition is studied and the limitation that single special clay is added to glaze batch is broken. It is proved that using ordinary ceramic raw materials to produce oil-drop glaze is feasible if the chemical composition of glaze is reasonable and the technology control is suitable. In addition, through the experiment it is found out that proper firing rule is the critical factor that affects the oil-drop glaze decorating effect. At the same time, other technological factors, for example, the thickness of glaze layer, glazing method, the size of glaze particle, which can affect the quality of glaze surface, must be considered.

晶体的适当熔解促使油滴形状圆整、尺寸大小适中,并在晶体表面形成一层玻璃相,有利于釉面光泽度的提高;通过正交试验方法探讨了油滴釉合理的化学组成范围,并突破了传统油滴釉制品及目前大多数仿制品采用单一特定天然釉土配料的局限,试验证明,只要化学组成合理,工艺控制适当,使用普通制瓷原料生产油滴釉制品是完全可行的;此外,本研究还发现,合理的烧成工艺控制是影响油滴釉装饰效果的关键性因素,同时还应注意釉层厚度、施釉方法、釉料研磨细度等其他工艺条件对釉面质量的影响。

Based on combination weighting analysis, fuse invalidation diagnosis technology consists of rule layer importance analysis technology, which includes establishing recurrent step layer, establishing contrast matrix, calculating weighting and checking consistency; security importance analysis technology, which includes establishing contrast matrix, calculating weighting and checking consistency; system combination vector analysis technology, which includes calculating system combination vector and checking system combination vector analysis technology.

作者:李科伟,王新政作者单位:(河南江河机械有限责任公司产品研究所,河南平顶山 467337)摘要:基于组合权重分析的引信失效诊断技术,包括由构造递阶层次、构造成对比较矩阵、计算权重及一致性检验等步骤组成的准则层重要度分析技术;由通过构造成对比较矩阵、计算权重及一致性检验得以完成的安全性重要度分析技术;以及由计算系统组合权向量和系统组合一致性检验两步构成的系统组合权向量分析技术。

Based on combination weighting analysis, fuse invalidation diagnosis technology consists of rule layer importance analysis technology, which includes establishing recurrent step layer, establishing contrast matrix, calculating weighting and checking consistency; security importance analysis technology, which includes establishing contrast matrix, calculating weighting and checking consistency; system combination vector analysis technology, which includes calculating system combination vector and checking system combination vector analysis technology.

作者:李科伟,王新政分类:装备与武器作者单位:(河南江河机械有限责任公司产品研究所,河南平顶山 467337)摘要:基于组合权重分析的引信失效诊断技术,包括由构造递阶层次、构造成对比较矩阵、计算权重及一致性检验等步骤组成的准则层重要度分析技术;由通过构造成对比较矩阵、计算权重及一致性检验得以完成的安全性重要度分析技术;以及由计算系统组合权向量和系统组合一致性检验两步构成的系统组合权向量分析技术。

Based on combination weighting analysis, fuse invalidation diagnosis technology consists of rule layer importance analysis technology, which includes establishing recurrent step layer, establishing contrast matrix, calculating weighting and checking consistency; security importance analysis technology, which includes establishing contrast matrix, calculating weighting and checking consistency; system combination vector analysis technology, which includes calculating system combination vector and checking system combination vector analysis technology.

作者:李科伟,王新政分类:装备与武器上传者:ZCOM网友作者单位:(河南江河机械有限责任公司产品研究所,河南平顶山 467337)摘要:基于组合权重分析的引信失效诊断技术,包括由构造递阶层次、构造成对比较矩阵、计算权重及一致性检验等步骤组成的准则层重要度分析技术;由通过构造成对比较矩阵、计算权重及一致性检验得以完成的安全性重要度分析技术;以及由计算系统组合权向量和系统组合一致性检验两步构成的系统组合权向量分析技术。

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