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The PCR products were labeled with the fluorescent DNA dye SYBR green. The amount of burden or load was measured by GeneAmp 5700 Sequence Detection System. Using this method, the HIV 1 proviral burdens in PBMC of patient and in cell suspension treated with the compounds AZT, GL and WT were measured.

以病毒感染细胞和培养上清为材料,测定了三种化合物对细胞内的前病毒载量和培养上清中的病毒载量的抑制活性,并与合胞体形成抑制方法测定化合物抗病毒活性的结果进行了比较。

Comparison of different types of stem cutting in rooting induced and analysis the cDNA clones which may be associated with the occurrence of adventitious roots of P.euphratica,the model of ARF were initial establishment of exogenous IBA controlling endogenous IAA occurred threshold and equilibrium,that is,exogenous IBA receiving by receptor of stem cutting,and transfer into the cells,then turn into active IAA by the effects of the POD enzyme.

通过对胡杨3个不定根发生调控培养体系的比较和与胡杨不定根发生可能相关的差异基因的克隆分析,初步建立IBA调控胡杨不定根发生的内源IAA阈值和激素间平衡的模型,即外源IBA通过插穗受体接收,转入细胞内并在POD酶作用下转化成具有活性的IAA,当IAA的水甲在促根的阈值内并保持相对平稳时,则有利于生根;当IAA的浓度增高超过阈值且不稳定时,则抑制不定根的发生。

Setting high speed counter, the changed angle of positioner rotation is converted to pulse number by encoder. Via comparison and judgment, the manipulator and positioner are realized real-time control. Thereby, the intelligent control of welding teaching and playback, seam tracking are achieved.

设置高速计数器,利用编码器将变位机转动的角度变化转换成脉冲数的多少,通过比较和判断,对操作机和变位机进行实时控制,从而实现焊接示教再现和焊缝跟踪的智能控制。

Method]Isolated bMSCs were cultured in vitro. rh Fibroblast Growth Factor 1(rhFGF-1)、rh Transforming Growth Factor-β1(rhTGF-β1)、rh Insulinlike Growth Factor-Ⅰ were utilizated. The proliferation of cells was detected by MTT assay, and the macroscopic histology , HE staining and immunohistochemical examinations were performed to seek the best cell factor. In vivo , to investigate the repair of the articular cartilage, bMSCs combined with fibrin glue and rhTGF-β1、rhIGF-I was compared with control group.

方法]rhFGF1、rhTGF-β1、rhIGF-I单独或联合应用对骨髓基质干细胞进行体外诱导培养,应用常规染色、MTT、免疫组织化学染色的方法筛选诱导骨髓基质干细胞成软骨细胞的最佳细胞因子,并将其与骨髓基质干细胞复合于纤维蛋白凝胶制成凝胶复合物,直接种植到兔膝关节实验性关节软骨缺损处,并与对照组相比较,观察软骨修复效果。

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推荐网络例句

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。