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Methods: During the April 2003 to December 2004, from the patients with different position colorectal cancer beyond Dukes C stage who were performed laparoscopic radical operation random draw 20 patients as experiment group, and from patients with different position colorectal cancer beyond Dukes C patients who were performed open radical operation 20 patients took randomly as contrast group. We draw thepatients blood about 5 ml from antecubital vein at the time before and after the operation about 30 minutes respectively, preserved the blood in deep hypothermia circumstance,cytrokeratin-20(CK-20) as the micrometastasis maker, using the RT-PCR technique,G3PDH as the intra-contrast gene, quantitatively detect the amounts of CK-20 in the blood with the gelatum imagery system and the computer analyzing software on the gelatum imagery system ,in order to compare the influence of the laparoscopic and the open operation on the micrometastasis.

在我院2003年4月—2004年12月间不同部位的临床分期在Dukes C期之内的接受了腹腔镜结直肠癌根治术结直肠癌患者中随机抽取20例作为试验组,另外在同期的接受开腹手术患者中随机抽取20例作为对照组,分别抽取术前30min和术后30min的肘前静脉血5ml,集中低温保存,以细胞角蛋白(cytekeratin-20)CK-20为微转移的标志物,应用RT-PCR技术,以G3PDH为内对照基因,应用凝胶成像系统及其计算机凝胶成像系统分析软件来定量检测手术前、后患者外周静脉血中CK-20含量,比较腹腔镜和开腹的手术方式对肿瘤微转移的影响。

I will compress them within the narrowest compass they will bear, staling the general principle, but not all its limitations. Equal and exact justice to all men, of whatever state or persuasion, religious or political; peace, commerce, and honest friendship with all nations, entangling alliances with none; the support of the State governments in all their rights, as the most competent administrations for our domestic concerns and the surest bulwarks against antirepublican tendencies; the preservation of the General Government in its whole constitutional vigor, as the sheet anchor of our peace at home and safety abroad; a jealous care of the right of election by the people--a mild and safe corrective of abuses which are lopped by the sword of revolution where peaceable remedies are unprovided; absolute acquiescence in the decisions of the majority, the vital principle of republics, from which is no appeal but to force, the vital principle and immediate parent of despotism; a well-disciplined militia, our best reliance in peace and for the first moments of war, till regulars may relieve them; the supremacy of the civil over the military authority; economy in the public expense, that labor may be lightly burthened; the honest payment of our debts and sacred preservation of the public faith; encouragement of agriculture, and of commerce as its handmaid; the diffusion of information and arraignment of all abuses at the bar of the public reason; freedom of religion; freedom of the press, and freedom of person under the protection of the habeas corpus, and trial by juries impartially selected.

我打算尽量简略地加以陈述,只讲一般原则,而不讲其全部范畴:不管人们的地位、宗教信仰或政治主张有何不同,人人都应得到平等和绝对公正的待遇;与所有国家和平相处,互相通商,并保持诚挚的友谊,但不与任何国家结盟,以免纠缠不清;维护各州政府的一切权利,使它们成爲处理我们内政最合适的机构,以及抵制反共和趋势的最有力的屏障;维护全国的政府,使之能充分行使宪法赋予的权力,从而成爲对内和平和对外安全的最后堡垒;要十分注意维护人民的选举权,因爲革命留下的弊端,一时没有和平的补救办法,而人民选举权乃是对那些弊端的一种温和而安全的矫正手段;要绝对服从多数的决定,这是共和政体的主要原则,离开这个原则,便只好诉诸武力,而这就是专制的主要原则和直接起源;要维持一支纪律严明的民团,以作爲和平时期和战争初期的最好依仗,直至正规军来接替;实行文权高于军校的政制;节省政府开支,减轻劳动人民的负担;如实偿还我们的债务,把维护政府的信用看作神圣的义务;促进农业发展,并鼓励以商辅农;传播知识并以公衆理智爲依据谴责一切弊端;保障宗教自由及出版自由,并以人身保护令以及由公平选出的陪审团进行审判来保障人身自由。

Objective To investigate the expression and significance of basophilic fibroblast growth factor during hepatocarcinigenesis in the rats.

目的 探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)在肝癌发生过程中的表达及意义方法采用免疫组织化学ABC法对二乙基亚硝胺诱发大鼠肝癌发生过程中碱性成纤维细胞生长因子表达情况进行观察。

Discussion on membrane formation mechanismBased on the Flory-Huggins theory, Phase equilibrium equations of PVDF/DMAc/water ternary system were wrote out, and the binodal and spinodal lines in the phase diagrams of the system were obtained via numerical calculation. Combined the kinetic description and the thermodynamic phase diagram, membrane formation process were analyzed, which prepared the theoretical foundation for the further membrane formation mechanism research.

论文主要从如下四个方面展开:(1)PVDF疏水膜成膜分相机理探讨基于Flory-Huggins理论推导了三元制膜体系凝胶分相组成的线性表达式,并通过&浊点法&测定了PVDF/N,N-二甲基乙酰胺/水/成膜体系的凝胶边界线组成,通过对实验数据的拟合,得到了简捷直观的线性表达式,ln(φ_1)/(φ_3)=bln(φ_2)/(φ_3)+a,误差小于3%。

On the other hand, extract of cemental matrix was separated and clarified from healthy teeth which were extracted because of orthodontic treatment.

从因正畸需拔除的健康牙体表面获得牙骨质基质提取物,分别观察不同作用时间、不同浓度的牙骨质基质提取物对牙龈成纤维细胞和牙周膜成纤维细胞在牙体表面附着的影响。

Results: The extract of cemental matrix could enhance the initial attachment of human gingival fibroblasts and human periodontal cells to the surface of root, and such effects were strengthened with increasing density and time.

且牙骨质基质提取物促牙周膜成纤维细胞附着的作用高于对牙龈成纤维细胞的作用。

Analyse and compare what adopt a variety of pair of methods, the Michael addition reaction of ester of acerbity second of second of hydroxyl of protection of benzyl of the response of close nucleus addition that uses second nitrile and ester of formic acid second certainly, chloridize, cyanogen, reaction that take off carboxyl generates 3- benzyl oxygen radical the fifth of the ten Heavenly Stem the method of 2 nitrile, detailed discussion the action that wait and the by-product that arise possibly reach the reactant in each pace reaction, activator, dissolvent avoid a method.

通过对多种方法的分析和比较,确定采用乙腈和甲酸乙酯的亲核加成反应、氯化苄保护羟基、氰乙酸乙酯的Michael加成反应、脱羧反应生成3-苄氧基戊二腈的方法,并具体的讨论了每一步反应中反应物、催化剂、溶剂等的功能和可能产生的副产物及避免方法。

Objective To compare the growth and proliferative characteristic of cultured fibroblast cells taken from pterygial and normal conjunctival specimens.

目的 对比观察相同的培养条件下翼状胬肉与正常结膜成纤维细胞的生长增殖状况,对两种成纤维细胞的生长增殖特性作出评价。

AIM: To investigate the effects of dibairen decoctum on the proliferation of keratinocytes and fibroblasts as well as the potential effects of DBRD on fibroblast contraction.

目的:观察地白忍煎剂对角朊细胞和成纤维细胞增殖的影响以及对成纤维细胞收缩功能的潜在作用。

Through the magnetic separation,acidwashing,sieving,reducing bleaching deferrization ,the whiteness of product can be improved,and the reaction mechanism and charging system discussed.

本文中采用的是烧成白度为80.1%的产品为研究对象,通过磁选、酸洗、过筛、还原漂白除铁等方式提高产品的烧成白度,并对不同的试验研究和反应机理进行探讨。

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