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In this thesis, we developed chiral proton N-O type ligands and employed them in the enantioselective addition of diethylzinc to N-diphenylphosphinoyl imines. The relationship between the structure of the ligands and the enantioselectivity was systematically studied. Furthermore, enantioselective butylation of N-diphenylphosphinoyl imines withBu〓Zn and enantioselective diphenylzinc addition to imine were also examined.

本文致力于亚胺的不对称催化有机锌试剂加成反应的研究:以手性质子型N-O配体为促进剂,对N-二苯基次膦酰亚胺进行不对称乙基锌加成反应而展开研究工作,系统地考查了手性配体的结构对反应的影响;另外,对N-二苯基次膦酰亚胺的不对称催化丁基锌加成进行了研究,并首次对亚胺的不对称苯基锌加成反应进行了初步探索。

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果:(1)在无血清条件下单独培养的DRG,背根神经节的轴突数目众多,外形纤细弯曲,不成束,并且施万细胞和成纤维细胞稀少,所以可以排除两者对轴突生长的影响,为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件;(2)在无血清条件下DRG和变性坐骨神经段联合培养,①先单独培养DRG,4天后待神经元轴突长出,再与坐骨神经段联合培养,观察到神经元的轴突数目减少,外形挺直,部分轴突之间相互粘附成束;②变性坐骨神经段和DRG同时联合培养,神经元的轴突数目明显减少,外形粗壮,轴突之间相互粘附成束;(3)有血清条件下单独培养DRG,轴突数目较多,外形挺直,长短不一,部分神经元的轴突之间相互粘附,施万细胞和成纤维细胞数目众多,观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

In the first step,through using the formula of the conformal transformation,a continuous medium with regulation boundary in original area could be transformed to a homogeneous medium with anomalous boundary in complex area,so the new constant velocity field could be more adapt to the current Stolt migration's conditions.

首先,利用保角变换法可将不规则边界的单连通区域转变成规则边界的原理,将规则边界的连续介质场转变成不规则边界的均匀介质场,使该均匀介质场更适应现有Stolt等偏移方法对速度场的要求;其次,对改变了区域的模型地震记录进行常规的偏移成像处理;再利用推导出的反变换公式对成像结果进行反变换,得出模型的真实偏移成像结果。

In this thesis, we has made research in ScanSAR mechanism and imaging theory, which is the base of studies on imaging radiometric precision and accuracy, including causation and correcting technology of ScanSAR azimuth scalloping distortion.

本文对星载ScanSAR的工作机制和成像原理进行了研究,对ScanSAR成像处理器辐射精度的影响因素进行了理论分析,并研究了影响ScanSAR 方位向成像精度的扇贝效应的成因、对成像辐射精度的影响及其校正技术。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体;2)利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达;3)构建ZNRD1的小干扰RNA载体,并测序鉴定;4)利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞(AGS、SGC7901、MKN28)和小鼠成纤维细胞(NIH3T3),G418筛选后进行鉴定;5)利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞(SGC7901)、正常胃组织上皮细胞(GES-1)、对长春新碱耐药的胃癌细胞(SGC7901/VCR)、药敏白血病细胞(HL-60)、对长春新碱耐药的白血病细胞(HL-60/VCR),G418筛选后进行鉴定;6)利用MTT实验检测ZNRD1高/低表达对细胞(AGS、SGC7901、MKN28、NIH3T3、GES-1)生长的影响;7)通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响;8)通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响;9)通过流式细胞仪分析ZNRD1高/低表达对细胞(AGS、MKN28、NIH3T3、GES-1)的细胞周期的影响;10)通过流式细胞仪分析上调ZNRD1对细胞(AGS、MKN28、NIH3T3)的凋亡的影响;11)通过MTT实验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)体外药物敏感性的影响;12)通过肾包膜下移植法检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR)体内药物敏感性的影响;13)通过流式细胞仪分析ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60、HL-60/VCR)内阿霉素蓄积和泵出的影响;14)通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响;15)通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞(SGC7901、SGC7901/VCR、HL-60)凋亡敏感性的影响;16)通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响;17)利用RT-PCR、Western blot对基因芯片的结果进行鉴定;18)利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用;19)利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用;20)利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响;21)利用反义核酸技术抑制p21的表达;通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响;22)利用反义核酸技术抑制p27的表达;通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响;23)利用脂质体转染法上调Skp2的表达;通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响;24)利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响;25)利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响;26)利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响;27)利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞(SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR)多药耐药表型的影响;利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

It introduced the classical theory of bubble nucleation during the process of foam plastics , and presented the calculation formulas about the radius of critical nucleation , required Gibbs free energy and nucleation rate of some kinds of nucleation. It reviewed the development of classical theory of bubble nucleation , including the influence of free volume and oversaturation on bubble nucleation , and pointed out the existing deficiencies in explanation of classical theory of bubble nucleation to bubble nucleation of dynamic polymer melt.

介绍了泡沫塑料加工过程中气泡成核的经典理论,给出了几种成核方式的临界成核半径、所需克服的吉布斯自由能及成核速率的计算公式;评述了经典成核理论的发展,包括自由体积和气体过饱和度对气泡成核的影响,并分析指出了经典成核理论对动态聚合物熔体气泡成核解释存在的不足。

Shim current calculation When shim coils are excited by appropriate current, they can generate correcting magnetic field to compensate the imaging magnetic field unhomogeneity. Based on the indirect calculation method, we proposed a novel iterative optimization algorithm to calculate shim current. Because there is a direct proportion relationship between FID signal and magnetic field homogeneity, we use the intensity of FID signal as feedback information to the calculation. Shim current can be worked out in less than 3 minutes. This satisfied the requirement that active shimming should be performed in real time.

5匀场电流计算对匀场线圈施加适当的电流后才能产生校正磁场消除成像磁场的非均匀分量,本文在匀场电流间接计算方法的基础上,利用磁共振成像原理中磁场均匀性与系统FID信号强度成正比的关系提出了一种电流迭代优化算法,将系统FID信号强度的变化作为反馈信息,能够快速计算出匀场电流对成像磁场进行补偿校正,平均耗时不到三分钟,满足磁共振成像系统对有源匀场的实时性要求。

Shim current calculationWhen shim coils are excited by appropriate current, they can generate correcting magnetic field to compensate the imaging magnetic field unhomogeneity. Based on the indirect calculation method, we proposed a novel iterative optimization algorithm to calculate shim current. Because there is a direct proportion relationship between FID signal and magnetic field homogeneity, we use the intensity of FID signal as feedback information to the calculation. Shim current can be worked out in less than 3 minutes. This satisfied the requirement that active shimming should be performed in real time.

5匀场电流计算对匀场线圈施加适当的电流后才能产生校正磁场消除成像磁场的非均匀分量,本文在匀场电流间接计算方法的基础上,利用磁共振成像原理中磁场均匀性与系统FID信号强度成正比的关系提出了一种电流迭代优化算法,将系统FID信号强度的变化作为反馈信息,能够快速计算出匀场电流对成像磁场进行补偿校正,平均耗时不到三分钟,满足磁共振成像系统对有源匀场的实时性要求。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

In summary, various key experimental conditions in electron acoustic imaging of ferroelectric domains were systematically studied. The optimum experimental conditions to obtain EAI of domains with good quality and the factors important to image analysis have been presented. The piezoelectric vibrator model and calculation of motion equation are helpful for explaining the imaging mechanism and related experimental phenomena. The sample holder with variable electric field was designed. The domain movement of BaTiO〓 ceramics and crystal under lateral and longitudinal applied electric field and domain movement of PMN-PT crystal under longitudinal poling electric field were observed. The application of SEAM on revealing of ferroelastic domains, imaging of residual stress distribution in ceramics coatings and around the pores on the surface of ferroelectric ceramic composite have been reported respectively. All these works enriched the study of electron acoustic imaging and extended the application of SEAM in the characterization of materials.

综上所述,本文系统地研究了各项关键实验条件对电畴电声成像的影响,指出了获得最佳畴结构电声像的条件和成像分析中应当考虑的各种影响因素;提出了解释电畴电声成像的压电振子模型,在此基础上推导并求解了压电振子运动方程,并对一些实验现象作出了解释;成功地设计了可变电场的样品台,观察了BaTiO〓陶瓷和晶体在横向电场作用下的畴运动,PMN-PT晶体在纵向极化电场作用下的畴运动;探索了电声显微镜在铁弹畴成像,陶瓷涂层应力观察,陶瓷自然表面气孔周围应力成像的应用,这些研究,丰富了电声成像的学术内涵,拓展了电声显微镜的应用领域。

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