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METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.

在差速贴壁+Thy1.1抗体及补体纯化法的基础上,剪开大鼠颅骨,显露位于颅腔前方的嗅球,取出2只嗅球,在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织,保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层,剪成1 mm3小块分离获取单细胞悬浮液,调整细胞密度至1×107 L-1,接种在用poly-l-lysine包被的培养瓶或培养板中,于37 ℃、体积分数为5%的CO2培养箱中培养,第3天用无血清DMEM/F12培养基换液培养。

He was inspired by a classic puzzle of a box made of five plastic pieces that could be assembled in myriad ways.

他受到一款经典拼图游戏的启发,这个游戏是将五块塑料模样的部件拼成一个盒子,而组合的方式是无限的。

On the background of collision between India plate and Eurasia plate and uplifting of Tibet, the viscosity lower crustal flow induced crust thicken extensively, and driven upper middleup crust break into some big and small subblocks. The continental deformation features in SYR, including remotion of rigid subblock through the movement of huge slip faults and extensive crustal thickening, are not fit "Crustal thickening" mode or "Continental extrusion" mode.

川滇地区的大陆变形特征既存在广泛的地壳增厚现象,同时在下地壳塑性流动的驱动下,中、上地壳破碎成大小不一的块体,变形特征表现出整体刚性,因而不能简单地套用"地壳增厚"或"大陆逃逸"模式。

A composed rhomboid prism composed of two isotropic left-handed material prisms with an uniaxially anisotropic LHM plate,there is an angle between the interface of which and the optical axis, was designed.

设计了一种左手材料复合双棱镜,由两块各向同性左手材料棱镜与夹在其间的、其界面与光轴成一定的角度的单轴各向异性左手材料平板构成。

RNC equipment is composed of a number of different function boards, all the boards cooperate together to achieve the function of RNC.

RNC整机是由若干块具有特定功能的单板按照一定配置组合而成的,所有的单板协同起来共同完成RNC自身的功能。

The structure formed by piling up over 2,000 pieces of glass with different size and angle comes from the Chinese traditional roof of tile.

这个由2000多块大小不同、角度不一的玻璃叠加而成的结构,取意于中国传统的瓦片屋顶。

And a new method of image stabilization is presented in order to quickly solve the problem of the translational and rotational motion between image sequences.First,the translational motion is calculated and compensated based on the gray projection algorithm.

针对视频图像序列的非稳特性,研究了电子稳像算法中灰度投影算法和块匹配算法各自的不足之处,提出了一种快速补偿视频图像序列间平移及旋转运动的稳定成像算法。

I block the production of plant products can be used for sand, stone, fly ash, cinder, coal-Shek, slag, mineral sources, such as industrial waste slag processing into the new wall materials.

我厂生产的砌块机产品均可采用砂、石、粉煤灰、煤渣、煤杆石、矿渣、矿源渣等工业废料加工成各新型墙体材料。

He sawed the wood into three pieces.

他把木头锯成三块。

In the next step, the pieces are cleaned, sorted, and sawed into pieces.

在下一步的清理块、分拣、锯成碎片。

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The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因,如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务,你就做你的事好了。

Who is in possession of this?

这是谁的?