成块

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

Cut the fish fillet into the small chunks, marinate with salt for 25 minutes.

将鱼肉改切成小块，放入碗内，加盐腌上25分钟

Also, calculate the distance of a line, calculate the mid point of a line, calculate the mid point of an arc, convert radians to degrees and vice versa, send characters to the AutoCAD command prompt, add single and multiple-line text to a drawing, add a new layer, calculate the slope and angle of a line, switch to model and paper space, check for existence of a block, see if a block has attributes, search and replace block attributes/single-line text/multiple-line text, count/erase/highlight/de-highlight specified number of a specified object, etc, etc. Doing each function with straight Visual Basic would require several lines of code, but with CAD.OCX only a minimal amount of code is required!

此外，计算出的距离为限，计算中的点线，计算中点的一个弧形的，转换成弧度，以度，反之亦然，送字给AutoCAD命令提示中，增加了单剂量和多行文本，一绘画中，增加一个新层，计算出坡度和角度的一条线，开关，以模型和图纸空间，检查是否存在著一个座，看有没有座了属性，搜索和替换块属性/单线text/multiple-行文本，计数/擦除/高/解突出指定数量的指定对象，等，等做了每个函数与直Visual Basic中，将需要几行代码，但与cad.ocx只有极小量的代码需要！

Any two or more walls which are monolithic will be referred to as wall assembly.

任何两个或多个成整块式的墙称为墙的部件。

Also get online later addiction bubble Internet bar is commonplace, delayed many kongfu, expended many money, by accident not little learning, go to school graduated go to school computer, learn computer to feel pretty good at that time, the teacher tells us to still can learn what Windows Xp to feel at that time to our newcome is what thing so mysterious, just knowing later is an operating system, play those who play to be met casually, can be imagined so this is broken groom the class has much rubbish what also was not learning is type rate rose, became in the Internet bar later a small network canal, at that time 300 a month, because need not want money to get online,work very happily still..

后来也就上网成瘾泡网吧是常事了，耽误了不少功夫，费了不少钱，误了不少的学习，上学毕业了就学了电脑，学电脑当时觉得不错，老师给我们新来的讲我们还会学什么windows xp 当时觉得是啥东西这么神秘，后来才知道就是一个操作系统，随便玩玩就会的，所以可想而知这破培训班有多垃圾了啥也没学着就是把打字速度提高了，以后就在网吧当了个小网管，那个时候三百块一个月，干得还非常开心因为可以不要钱上网。。

The grisly relics are being sold at the horror attraction as part of a new feature on the 17th century Newgate Gaol.

这惊人 e5遗骸块共贩卖，用号这恐怖 e5吸引力，成做一款新花样 e5一部分，伫17世纪新门监狱遮。

Now I will try to partition our framework into functional pieces and get down to the nitty-gritty.

现在，我会尝试的功能块分割成我们的框架，并着手进行的事情的本质。

Though she's not so noble-minded like you, at least, the heart she gives me is complete and the only one, not can be cut into pieces to sell.

虽然她没你那么高尚，至少她给我的心事完整的，唯一的，而不是切割成好几块来卖的。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

In this experiment we find a rounded hypoecho tumor in arat liver and elastography is green and the score is 1. The pathologic outcome ispurulence of liver cells. It seen that obtusely damnify liver tissue in the surgery andthen infect local.

研究中发现一例肝组织中有一圆形低回声包块，弹性成像显示为绿色，评分为1分，病理结果为肝细胞化脓性变，考虑为手术过程中钝性损伤肝组织，继而局限性感染所致。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。