成块

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily, their source in human are plenty, own active proliferation and potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful, the standard technology is culture the cell-ball in centrifuge tube, the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer , but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

The Xiaoxinancha copper-gold deposit occurs in the upwarped zone rimming the Mesozoic volcanic basin, being of porphyry type; the Naozhi gold deposit occurs in the lifted block within the Mesozoic volcanic basin, belonging to porphyry epithermal transitional type; the Wufeng and the Ciweigou gold deposit, which are two epithermal deposits, exist in the fault zone within the Mesozoic volcanic basin.

小西南岔金铜矿床产于中生代火山岩盆地边缘的隆起带，属于斑岩型矿床；闹枝金矿床产于中生代火山岩盆地内的断隆块，属于斑岩-浅成热液过渡型矿床；五凤和刺猬沟金矿床产于中生代火山岩盆地内断裂带，属于浅成热液型矿床。

Hou's handle-less rolling pin, break it into pieces, smash them into skins and fill and fold the dumplings.

侯阿姨用手把面团揪成小块，成薄面皮，然后把馅儿放进去。

Formerly Anyang Forging Equipment Factory was established in 1956, is the country's fixed-point production forging hammer manufacturers, science and technology enterprises in Henan Province, Henan Province Association forging units and executive director of the China Association for forging unit Enterprise covers an area of 83 mu, with total assets of 100,000,006 yuan Bai°°, the company has a small and medium-sized pieces of machining, large machining, riveting forging, into equipment, machine repair plant and a total of five in the former Ministry of Machinery three types of planning within the Institute of forging hammer, air hammer country standards drafted by the Anyang Forging companies, enterprises, through the ISO9001 certification, air hammer, riveting machine two products were the only company in Henan Province mechanical products designed to protect the country issued BrandName the "register marks of origin", enterprise products have series of air hammer, electric-hydraulic hammer machine, electric-hydraulic hammer head, CNC Fully Hydraulic Die Forging Hammer, forging manipulator, closed single-point presses, many automatic forging press workers, metal shavings Press, metal balers, precision bar cutting machine, large forging presses, shearing machine, bending machine, such as 13 series, 56 specifications of products, which air hammer, riveting machines have won the "ú" and "provincial excellent" designation, the product best-selling domestic and exported to the United States, Britain, Germany, India, Pakistan, Vietnam, North Korea, the world's more than 50 countries and regions.

安阳锻压机械工业有限公司成立于1956年，是国家生产锻锤的定点生产厂家，河南省科技型企业、河南省锻造协会副会长单位和中国锻协常务理事单位，企业占地面积八十三亩，资产总额一亿零六佰捌拾万元，公司设有中小件机加工、大件加工、铆锻、成装、机修共五个分厂和一个在原机械部规划内的三类锻锤研究所，国家空气锤标准由安阳锻压公司起草，企业通过了ISO9001认证，空气锤、铆接机两种产品获河南省机械产品唯一一家国家颁发的旨在保护名优产品的&原产地标记注册&，企业生产的产品有系列空气锤、电液锤整机、电液锤动力头、数控全液压模锻锤、锻造操作机、闭式单点压力机、多工位自动锻造压力机、金属屑压块机、金属打包机、棒料精密剪断机、大型热锻压力机、剪板机、折弯机等十三个系列，五十六个规格产品，其中空气锤、铆接机曾分获&国优&和&省优&称号，产品畅销国内，远销美国、英国、德国、印度、巴基斯坦、越南、朝鲜等世界五十多个国家和地区。

Fold in the egg whites.Finely chopped shrimp. Mixed with egg white and pork fat.

服：把虾切成均匀的小块，拌上蛋清和猪油制做而成。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。