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戊醛

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Results: The autofluorescence of M. furfur and M. japonica standard trainsrespectively in 4th and 6th day are stronger than that in other days, andautofluorescence of colonies become weaker in an hour. Theautofluorescence of Malassezia colonies put in 3% glutaraldehyde, pH4.0HCL, 1280μg/mL and 64μg/mL fluconazol solution, 0.3% methylene blueseparately get stronger, and that in 10% KOH get stronger at beginning thenbecome weaker 2 days later. C. albicans, S. schenchii, T. rubrum, T.tonsurans, A. terreus, A. fumigatus cultured on SDA all haveautofluorescence, and which are weaker than that of Malassezia.

结果:糠秕马拉色菌和日本马拉色菌2株标准株的自发荧光强度分别在培养第4和8天时最强,菌落的自发荧光在1小时内逐渐减弱;3%戊二醛溶液、pH4.0盐酸溶液、1280μg/mL和64μg/mL氟康唑溶液、3%美蓝溶液处理后马拉色菌自发荧光都增强,10%氢氧化钾溶液中的马拉色菌在开始时荧光增强,2天后减弱;白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等在培养条件下有自发荧光,但都较马拉色菌弱。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌,接种在Dixon培养基32℃培养,分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光;分别用3%戊二醛溶液、pH4.0盐酸溶液、10%氢氧化钾溶液、1280μg/mL和64μg/mL氟康唑溶液、0.3%美蓝溶液处理后观察马拉色菌自发荧光的变化;选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

M ethods 96 used gastro scope were average to 2 group s and were sterilized by be marinated in 2%glutaradehyde fo r 20 m inutesand acidic oxidized electro lyzed water fo r 5 m inutes,respectively.Compare the sterilizing effect of the 2 group s.

临床污染的96条胃镜分为两组,分别采用2%戊二醛浸泡20 min、酸性氧化电位水浸泡5 min的方法消毒,比较2种消毒液对胃镜表面和内腔的消毒效果。

Methods Prepare conjugated antigen BSA-Aβ by coupling hapten Aβ1-42 to vector protein BSA with glutaraldehyde method.

用戊二醛法将半抗原Aβ1-42偶联于载体蛋白BSA,形成结合抗原BSA-Aβ。

Penicillinase, hematein and nano gold colloid were immobilized,as a modified membrane,on a plane Pt electrode by glutaraldehyde-bovine serum albumin.

用戊二醛-牛血清白蛋白交联法将青霉素酶、纳米金胶和氧化苏木精同时修饰在铂电极上,制成电流型电化学生物传感器。

The results indicated that Fructose 1,6-bisphosphate aldolase,succinate dehydrogenase ,and hexokinase were very sensitive to temperatures .Citrate-synthase was similar to that of mesophiles in temperature properties .

结果表明Y18细胞中1,6-二磷酸果糖醛缩酶,琥珀酸脱氢酶和己糖激酶对温度很敏感,柠檬酸合成酶的温度效应类似于中温酶,α-酮戊二酸脱氢酶和异柠檬酸脱氢酶存在不同温度特性的同功酶。

If glutaraldehyde is used, all immersible internal and external surfaces should be in contact with the dis- infectant for not less than 20 minutes to achieve high-level disinfection.

如果使用戊二醛,所有能浸泡的内外表面应与消毒剂接触至少20分钟以达到高水平消毒。

The experimental group was given an operation of cutting infundibulum and the contol group was given an exposure of not cutting the infundibulum.Both groups were raised under similar condition.After surviving for 30 days,the animals were killed and specimens from aorta were taken and observed under light and electron microscope.

实验组行手术切断漏斗,对照组行手术暴露但不切断漏斗,手术后2d,两组动物移至同一条件下饲养,存活30d后用1%多聚甲醛+1.25%戊二醛液从心脏灌流固定,取主动脉作光、电镜观察。

Result The ironrusty size on ironwork is biggest in water, secondly in glutaral, last in 75% alcohol.

结果 铁制品组锈斑从轻到重依次为75%酒精、戊二醛、水。

Ironwork was immersed in water, 75% alcohol, JianZhiXu and liquid glutaral for 40 days. Endoscope' skin and Supply-water-pipe were immersed in 150 days.

用水、75%酒精、健之素、戊二醛溶液持续浸泡铁制品40d,外皮和送水管各150d,与未经浸泡者进行比较。

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