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Study on the Crystallization Process of Labeled GL-7-ACA Acylase CA130 Complex 7β-bromoacetyl amino cephalosporanic acid (BA-7-ACA), an analog of glutaryl-7-amino cephalosporanic acid (GL-7-ACA), can inhibit and specifically alkylate GL-7-ACA acylase (CA130) from Pseudomonas sp. 130, forming a carbon-carbon bond between BA-7-ACA and the C-2 on indole ring of Trp-β4 residue of CA130.Here we reported that BA-7-ACA labeled CA130 (BA-C130) could self-catalyze the hydrolysis of BA-7-ACA during crystallization process. The hydrolysis was confirmed to be a reaction analogous to the one of GL-7-ACA by comparative MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) spectrometry analysis.

二、GL-7-AcA酰化酶CA130标记复合物的结晶过程研究溴乙酰氨基头孢烷酸(7β-bromoacetyl amino cephalosporanic acid,BA-7-ACA)作为戊二酰-7-氨基头孢烷酸(GL-7-ACA)的类似物,不仅能够抑制GL-7-ACA酰化酶CA130的活力,而且能通过在BA-7-ACA和CA130的β亚基第四位色氨酸吲哚环二位碳原子之间形成碳-碳共价键而将CA130特异的烷基化。

By the theories of cavitations and free volume, it analyses the factors on lubricant low-temperature property in view of molecule structure, brings up effective ways to improve the low-temperature properties, conducts plenty of testing with even designs, and then investigates and tests the lubricant's low-temperature properties with computerized system; it analyses the data by regressi on method and optimizes the solving, and studies on the base oil of environment friendly lubricants and selects the composition of colza oil, esters and PAO2, and discusses on low-temperature flowability of vegetable-base lubricants.

应用空穴理论及自由体积理论,从分子结构角度分析了影响润滑油低温性能的因素,提出了改善对润滑油低温性能的有效途径,组织了大量试验,通过润滑油的计算机检测系统对润滑油的低温性能进行了分析检测,采用均匀设计来组织实验;用逐步回归对数据进行分析并且进行最优化求解,对环境兼容润滑油的基础油进行了分析研究,选择菜籽油、季戊四醇酯和聚α-烯烃作为基础油,并从理论上对植物基润滑油的低温流动性进行了探讨。

By the theories of cavitations and free volume, it analyses the factors on lubricant low-temperature property in view of molecule structure, brings up effective ways to improve the low-temperature properties, conducts plenty of testing with even designs, and then investigates and tests the lubricants low-temperature properties with computerized system; it analyses the data by regression method and optimizes the solving, and studies on the base oil of environment friendly lubricants and selects the composition of colza oil, esters and PAO2, and discusses on low-temperature flowability of vegetable-base lubricants.

应用空穴理论及自由体积理论,从分子结构角度分析了影响润滑油低温性能的因素,提出了改善对润滑油低温性能的有效途径,组织了大量试验,通过润滑油的计算机检测系统对润滑油的低温性能进行了分析检测,采用均匀设计来组织实验;用逐步回归对数据进行分析并且进行最优化求解,对环境兼容润滑油的基础油进行了分析研究,选择菜籽油、季戊四醇酯和聚α-烯烃作为基础油,并从理论上对植物基润滑油的低温流动性进行了探讨。

By the theories of cavitations and free volume, it analyses the factors on lubricant low-temperature property in view of molecule structure, brings up effective ways to improve the low-temperature properties, conducts plenty of testing with even designs, and then investigates and tests the lubricant's low-temperature properties with computerized system; it analyses the data by regression method and optimizes the solving, and studies on the base oil of environment friendly lubricants and selects the composition of colza oil, esters and PA02, and discusses on low-temperature flowability of vegetable-base lubricants.

应用空穴理论及自由体积理论,从分子结构角度分析了影响润滑油低温性能的因素,提出了改善对润滑油低温性能的有效途径,组织了大量试验,通过润滑油的计算机检测系统对润滑油的低温性能进行了分析检测,采用均匀设计来组织实验;用逐步回归对数据进行分析并且进行最优化求解,对环境兼容润滑油的基础油进行了分析研究,选择菜籽油、季戊四醇酯和聚α-烯烃作为基础油,并从理论上对植物基润滑油的低温流动性进行了探讨。

Furthermore, the important fragment which is composed of pentenyl cinnamic acid and D-Tyrosine, and the ester which is composed of L-N-methylated phenyl amino acid and diketopiprazine was synthesized with considerable yield The conjugation of the new DOPA analogue into the peptide in the total synthesis of Pepticinnamin E was investigatited

另外,本文作者还进一步合成了高收率的戊烯基肉桂酸衍生物与D-酪氨酸的缩合片段及L-N-甲基苯丙氨酸与哌嗪二酮的酯两个重要的结构组成部分;对新多巴衍生物片段嵌入肽链中的工作亦进行了有益的尝试。

The specific properties of materials, such as the palate adhesion, dynamic cruor, anti-bacterial and the degradability of lysozyme, were studied, which provide valuable data for further application. The main creative ideas are listed as follows:(1) The water-retention chitosan/poly vinyl alcohol blend fiber was obtained by coagulating in 10% NaOH/ethanol solution, and the blend and crosslink due to GA can enhance the mechanical properties of fiber;(2) The controlled degradable chitosan/gelatin blend fiber was obtained by solution filature, and the mechanical properties were improved;(3) The N-acylchitosan with the different degree of substitution were successfully prepared, and the relationship between structure and properties was estabilished;(4) By a new solvent system of 6% NaOH/4% urea for cellulose, the blood anti-coagulant function films were prepared by blending cellulose with chitin;(5) In the same solvent system above mentioned, the anti-bacterial blend films based on cellulose and carboxymethyl-chitosan were prepared, and the relationship of DS and the anti-bacterial property was concluded;(6) The controlled degradable chitosan films was obtained by the chemical crosslink.

本论文的主要创新点:(1)以NaOH水溶液/无水乙醇为凝固液,采用溶液纺丝法制备出保水性壳聚糖/聚乙烯醇纤维,共混和戊二醛交联极大提高了壳聚糖纤维力学性能;(2)用壳聚糖和明胶共混,以溶液纺丝法制备出可控降解的壳聚糖/明胶纤维,并大幅度提高了壳聚糖共混纤维的干、湿态抗张强度;(3)确立了不同取代度N-酰基壳聚糖的合成方法及其膜的结构与性能的关系;(4)利用纤维素新溶剂6%NaOH/4%尿素制备出抗凝血性纤维素/甲壳素共混膜;(5)利用上述纤维素新溶剂制备出抗菌性纤维素/羧甲基壳聚糖共混膜,揭示了羧甲基壳聚糖取代度与抗菌性能的关系;(6)制备出可控降解壳聚糖交联膜。

Analyse and compare what adopt a variety of pair of methods, the Michael addition reaction of ester of acerbity second of second of hydroxyl of protection of benzyl of the response of close nucleus addition that uses second nitrile and ester of formic acid second certainly, chloridize, cyanogen, reaction that take off carboxyl generates 3- benzyl oxygen radical the fifth of the ten Heavenly Stem the method of 2 nitrile, detailed discussion the action that wait and the by-product that arise possibly reach the reactant in each pace reaction, activator, dissolvent avoid a method.

通过对多种方法的分析和比较,确定采用乙腈和甲酸乙酯的亲核加成反应、氯化苄保护羟基、氰乙酸乙酯的Michael加成反应、脱羧反应生成3-苄氧基戊二腈的方法,并具体的讨论了每一步反应中反应物、催化剂、溶剂等的功能和可能产生的副产物及避免方法。

The contact angle of glassfiber epoxy resin fluoridized by 3-(1H, 1H, 5H-Octafluoropentyloxy)-1, 2-Propenoxide hasincreased from 51.3°to 75.3°, and the surface tension has reduced from 36.5mN/m to25.5mN/m accordingly.

同时经过环氧丙基八氟戊醚处理的环氧玻璃钢表面对水的接触角也由原来的51.3°增大到75.3°,其表面张力由36.5mN/m减小到25.5mN/m,达到一定的疏水疏油效果。

It is found that compared with the untreated glass fiber reinforced unsaturated polyester, the fluoridized FRP"s property of anti-water and anti infirm-acid has enhanced, while theproperty of anti- strong acid, anti-strong alkali and anti-organic solvent hasn"t changed much.The property of anti-acid and anti-alkali of the glass fiber reinforced epoxy resin fluoridizedby 3-(1H, 1H, 5H-Octafluoropentyloxy)-1, 2-Propenoxide has a corresponding increase, but theproperty of anti-organic solvent hasnt changed much.

研究发现,经G-02、G-04处理后的聚酯玻璃钢的与未处理的聚酯玻璃钢相比,其耐水性增强,耐弱酸性也有所增强,但耐强酸性、耐碱性、耐有机溶剂的腐蚀性变化不大;而经环氧丙基八氟戊醚处理后的环氧玻璃钢其耐酸耐碱性都有相应的增强,但是其耐有机溶剂的腐蚀性无太大变化。

The results of the TG and DTG curves show that, in the whole intumescing process, APP, PER and MEL cooperate with each other and synergism is generated; The kind of base resin and its contents, the ratio of the component of fire retardant system and their contents all have great influence on the thermal degradation behaviors of fire retardant coating; The concentration of oxygen has no influence on the thermal degradation of fire retardant coating below 345℃, while the influence can not be neglected above 345℃, but compare with the factors mentioned previously, the influence is small.

研究表明,聚磷酸铵-三聚氰胺-季戊四醇三者能密切配合,对阻燃起到协同作用;基料树脂的种类及含量、阻燃体系配比及含量对防火涂料的热分解行为有较大的影响;氧气浓度在低温阶段(345℃以前)对热解行为几乎没影响,而在高温阶段(345℃以后)则对热解行为有影响,但相对于前面的因素对热解的影响来说,氧气浓度对防火涂料的热解行为影响较小。

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