感染上...

Expression of CD44V6 can be an early biological indicator of precarcinomatous epithelial cells. Gastric mucosa intestinal metaplasia can induce the expression of CD44V6, moreover, Hp infection can promote it.

CD44V6的表达可能是上皮细胞癌前病变出现的早期生物学信号，肠上皮化生细胞可能诱导CD44V6的表达，而Hp感染则有促进这种诱导表达的作用。

An unusual isolate from watercress mottle,in association withturnip mosaic and cucumber mosaic viruses,produced small white local necrotic lesions on sapinoculated leaves of Nicotiana tabacum,N.rustica,N.glutinosa,Datura stramonium,Solanumnigra,Brassica pekinensis,B.chinensis,B.campestris,Raphanus sativa,Nasturtium officinale,Spinacia oleracea and Amaranthus mangostanus while small brown local necrotic lesions onGomphrena globosa,Phaseolus vulgaris,Dolichos lablab...

在北京香山樱桃沟野生的十字花科豆瓣菜上发现有芜菁花叶病毒、黄瓜花叶病毒以及一种未报导过的&小白斑&病毒的复合病。小白斑病毒在20多种寄主上，包括十字花科，单独汁液接种时只能产生局部坏死病斑，不能系统侵染；单独接种而能系统感染的寄主有百日菊，只产生极轻微的深浅绿花叶，甚至有时几乎无病状。小白斑病毒能在复合病情况下系统存在于豆瓣菜中是值得注意的一种现象。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

Artistically, using the creation method of describing mental activities can expand topics and deepen the characterization of heart and soul to make infection and education. Educationally, using the methods of suggest podia can stimulate one's potential to improve education benefits.

在艺术上，运用暗示心理描写的创作手法，可以扩大题材，深入地刻画人的心灵，给人以感染和教育；在教育上，暗示教学法能调动人的潜在能力，调动人的无意识活动的精神能动性，大大提高教育效益。

Methods 156 subjects were randomized into two groups: treatment group in which 79 cases were treated by aerosol inhalation of Chuan Ke Zhiplus routine therapy and control group in which 77 cases were treated by Pulmicort Res pules plus routine therapy, wit

方法选择急性喘息性支气管炎及哮喘急性发作的患儿156例，随机分为治疗组（79例，在常规抗感染止咳的基础上使用喘可治加可必特氧气驱动雾化吸入）和对照组（77例，在常规治疗的基础上加用普米克令舒及可必特氧气驱动雾化吸入），两组其他治疗相同，疗程均为1周。

The candida albicans and candidas tropicalis was more common in clinical samples, and the positive rate of drug resistant strain against fungus increased gradually. So it is important to culture, isolate and identify the fungus from clinical samples, and do the antifungal sensitivity at the same time.

临床上酵母样真菌感染以白色念珠菌和热带念珠菌较常见；真菌耐药菌株的检出率呈上升趋势，临床上必须重视真菌的检出和药敏试验。

Results: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms （purple-blue, NBP-BCIP.

原位杂交和免疫组化结果显示：支气管上皮细胞、Ⅱ型肺泡上皮细胞、血管内皮细胞、巨噬细胞、纤维母细胞及T淋巴细胞在所有SARS病例中都受到了病毒感染。

Abstract］ Objective To investigate the change of bronchial mucosa cilia epithelium structure on children with recurrent respiratory tract infection in acute stage and convalescence stage.Methods Choose 38 cases of patients with RRTI as study group,respiratory ciliated cells were collected by bronchial fiberscope in acute stage and convalescence stage respectively,observe the change of cilia structure by electron microscopy and compare the incidence of cilia cylindrical epithelium structure abnormality.

摘要］ 目的研究反复呼吸道感染患儿急性期与恢复期支气管黏膜纤毛上皮结构的特点与变化，临床治疗的有效性提供客观依据方法选择符合儿童RRTI诊断标准的患儿38例作为研究对象，分别于急性期及恢复期在纤维支气管镜下夹取支气管黏膜组织，电镜下观察纤毛上皮结构的特点并比较纤毛异常的发病率，探讨RRTI患儿治疗后纤毛的恢复情况。

The PCR products were labeled with the fluorescent DNA dye SYBR green. The amount of burden or load was measured by GeneAmp 5700 Sequence Detection System. Using this method, the HIV 1 proviral burdens in PBMC of patient and in cell suspension treated with the compounds AZT, GL and WT were measured.

以病毒感染细胞和培养上清为材料，测定了三种化合物对细胞内的前病毒载量和培养上清中的病毒载量的抑制活性，并与合胞体形成抑制方法测定化合物抗病毒活性的结果进行了比较。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。