惊厥后的

Two hours later, the convulsant mice were anesthetized deeply and fixed by transcardiac perfusion for Immunohistochemistry to detect c-Fos expression.③ Comparison of neurotoxicity induced by CD50 of three agents.

将发生惊厥的小鼠妥善标记，2 h后制作冰冻冠状切片，检测神经元核蛋白c-Fos的表达，镜下计算不同区域表达c-Fos的棕黄色颗粒数目，每个颗粒代表1个神经元。

Days after a convulsant dose of KA, the seizure susceptibility were found in KA+NS group and enhanced in KA+PL017 group, whereas significantly decreased in KA+β-FNA group (p.01).β-FNA prolonged the latency and reduced the stage of seizures in dose-dependent manner.

结果显示，一次给予惊厥剂量KA后7天，KA+NS组的癫癎发作敏感性形成；MORs激动剂组（KA+PL017）动物出现癫癎发作敏感性增强；与上述两组比较，MORs拮抗剂组动物癫癎发作敏感性明显降低（p.01），β-FNA以剂量依赖方式延长癫癎发作的潜伏期、降低发作级别。

The seizure sensitive rats were made by subcutaneous injection of a convulsant dose (10mg/kg) of KA. Normal saline , PL017 or β-FNA were infused constantly into ventral hippocampus of awakened and freely moving animals for 7 days by mini-osmotic pumps. 7 days later, all rats were given subcutaneously with subconvulsant dose of KA.

皮下注射惊厥剂量（10mg/kg）的KA制备颞叶癫癎模型的癫癎发作敏感大鼠，采用微渗透泵技术分别将生理盐水、PL017和β-FNA连续7天恒速注入清醒自由活动的上述动物的腹侧海马内。7天后皮下注射阈下剂量（5mg/kg）KA检测癫癎发作敏感性形成情况。

The epilepsy model induced by kainic acid is a dynamical model condition. 5-7 days after acute seizure episodes induced by a single systemic injection of a convulsant dose (10mg/kg) of KA the rats developed a long-lasting increase in seizure susceptibility and the characteristic histopathological features in the hippocampus similar to human TLE. So the KA model has been a well-known model to study TLE or intractable epilepsy.

颞叶癫癎红藻氨酸（kainic acid，KA）模型具有明确的动态变化过程，一次全身性给予惊厥剂量（10mg/kg）KA诱发SD大鼠急性癫癎发作后5-7天，动物开始出现癫癎发作敏感性长期增强，脑内亦发生与人类颞叶癫癎相似的神经病理、神经生化及神经分子生物学方面的改变，因此被认为是研究颞叶癫癎和难治性癫癎的理想模型之一。

Objective To find out the changes of synaptic density in the periaqueductalgray of the naive and kindled audiogenic seizures-prone rats.

目的 分析正常及点燃后听源性惊厥易感大鼠中脑导水管周围灰质内突触密度的变化情况。

Methods The neuronalstaining method and the immunocytochemical method were used to identify the Fos-postive neurons in the PAG of the audiogenic seizures-prone rats(P77PMC) after audiogenic seizures.

用神经细胞染色和免疫细胞化学技术，观察听源性惊厥发作后易感大鼠(P77PMC)PAG内即早基因c-fos的表达情况。

The main peaks of the EEG power spectra were in delta and theta frequency range. In the stage of ethanol exposure, the rats exhibited much more act of attack than the control, the alpha waves of EEG decreased, while the delta waves increased. The EEG power spectra analysis showed the delta power increased and the theta power decreased. In the stage of ethanol withdrawal, the rats showed some withdrawal behaviors such as wet dog shakes, sniffing, groomingand tonic-clonic seizures for audiogenic stimulus. Spikes and spike-slow waves were found in the EEG. In power spectra analysis, the delta power decreased while the alpha and the theta power increased, the EEG frequency band moved to the high-frequency rang. These changes were found obviously on the sixth day and disappeared on the 1 Oth day after the ethanol was removed.

本研究发现：1、对照组大鼠行为无异常，伏核EEG以α节律为主，散在出现δ和θ节律；功率主峰在δ和θ频段范围，整个过程中未发现手术对伏核EEG及脑电功率谱有影响。2、在慢性饮酒过程中，大鼠攻击性行为增加，伏核 EEG的α节律明显减少，散在出现的高幅慢活动δ波逐渐增多，功率谱分析表明δ频段功率百分比明显增加，而θ频段功率百分比明显减少，总功率减少。3、停饮后，大鼠出现湿狗样抖动、打喷嚏等撤药综合症，并且高频声音刺激能引发惊厥；伏核EEG出现棘波和棘慢综合波，并持续增多，功率谱分析显示δ频段功率百分比 5 南京医科大学硕士学位论文逐渐减少，而0和以频段功率百分比逐渐增加，总功率增加。

Results By univariate analysis 11 risk factors were explored: family poverty, low culture level of mother, history of family on MR or epilepsy in parents, abnormal maternal menstrual cycle before pregnancy, febrile illess during gestation, bleeding in gestation period, chronic disease in gestation period, premature birth, assisted first crying after birth, neonatal febrile illness, convulsion and asphyxiation.

结果单因素分析儿童MR的危险因素有：家庭贫困、母亲文化程度低、父母亲智力低下或癫痫家族史、母亲妊娠前月经周期异常、母孕期发热、母孕期出血、母孕期患有慢性病、早产、出生后第一声啼哭是否需辅助、新生儿发热、惊厥、窒息。

In the current study, we evaluated the behavioral effects of two standard drugs used clinically for neuropathic pain, the anticonvulsant gabapentin and antidepressant imipramine, in rats at different times after peripheral nerve injury.

现在的研究，我们评估了两种神经性头痛临床治疗的一线药物在老鼠周围神经损伤后不同时间段行为效应，即抗惊厥药加巴喷丁和抗抑郁药丙咪嗪。

Methods: After intranasal or intraperitoneal administration of r-HuEPO, the behavioral and electroencephalographic changes were observed in pentylenetetrazol and maximal electroshock induced seizure or electrical amygdaloid-kindled seizure of rats.

另外，大鼠经鼻给r-HuEPO后，观察对最大电休克诱导的惊厥发作级别，前肢或后肢强直性伸展时间的影响；对杏仁核电点燃后阈刺激诱导的行为等级，大发作持续时间及后放电的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。