情况
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Cells were immediately fixed and processed for immuno?uorescence and tested for translocation ofβ-catenin.β-catenin and target genes of Wnt/β-catenin signaling COX-2, cyclinD1, c-fos, c-Jun mRNA expression were examined by RT-PCR. Saos-2 osteoblastic cells were co-transfected with a wild type or mutant Tcf luciferase reporter gene and Renilla luciferase plasmid. Posttransfection cells were subjected to 1 hour of 12% elongation.
作用1h、2h和4h后,用免疫荧光的方法检测β-catenin的表达和在细胞上定位的变化;用半定量RT-PCR检测β-catenin mRNA表达的变化;用质粒转染的方法检测荧光素酶报告基因Tcf转录的情况;用半定量RT-PCR检测Wnt/β-catenin通路下游基因COX-2,cyclinD1, c-fos, c-Jun mRNA表达的情况;用免疫共沉淀的方法检测β-catenin和E-Cadherin的结合情况。
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Methods Patients with nasopharyngeal who had survived ten years after radiotherapy were investigated by mesns of questionaire on difficulty in opening mouth,dysacusis,dysopia,open rhinolalia,cariogenesis and sexuality.Results 108 cases wereinvestigated.
以随访信的方式,填表调查生存十年以上的患者的生活情况,其中包括张口困难,听力障碍,视力障碍,口咽干燥,龋齿情况,语音情况,性生活状况等及其是否影响生活,由病人自我评价其生活质量。
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Among them,a strain saccharomycete Dfjm-1 with high flocc ulating activity was obtained.The microbial flocculant of Dfjm-1 produce is na med Dtjm-1.In this paper,time course of flocculants production including growt h,flocculating activity,and pH were studied.
本文研究了&Dfjm-1&在不同培养时间的生长情况、培养液中pH值变化情况及絮凝活性变化情况,得出絮凝活性与菌生长量呈正相关关系,絮凝剂&Dtjm-1&在菌生长稳定后期达到稳定活性的最高值。
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Much data such as indoor and outdoor temperature, the solar radiation, the core and surface temperature of Trombe wall , the temperature and velocity of vent airflow were recorded every ten minutes and saved in computer by a computerized data-logger device.
本研究在新建成的被动式太阳房内,利用多通道计算机巡回检测系统,对室内外温度、太阳辐射强度、集热蓄热墙体温度以及通风口风速、温度进行了长期实测,研究含有 Trombe墙体的新建被动式太阳房采暖季节的热性能,采暖季节的室内温度随室外气象条件以及太阳辐射强度的变化情况、室内温度在集热蓄热墙体作用下的波动情况以及在不同天气情况下 Trombe墙对室内温度的影响。
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The experiments were carried out on a new passive solar test cell. Much data such as indoor and outdoor temperature, the solar radiation, the core and surface temperature of Trombe wall, the temperature and velocity of vent airflow were recorded every ten minutes and saved in computer by a computerized data-logger device. Based on these data, this paper analyzed thermal performance of passive solar house under different weather situations. Meanwhile the effect of Trombe wall on the indoor thermal environment in heating season was also examined.
本研究在新建成的被动式太阳房内,利用多通道计算机巡回检测系统,对室内外温度、太阳辐射强度、集热蓄热墙体温度以及通风口风速、温度进行了长期实测,研究含有Trombe墙体的新建被动式太阳房采暖季节的热性能,采暖季节的室内温度随室外气象条件以及太阳辐射强度的变化情况、室内温度在集热蓄热墙体作用下的波动情况以及在不同天气情况下Trombe墙对室内温度的影响。
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P19 differentiation was induced by retinoic acid, and P19 cells cultured on gelatin were used as control. Differentiated cells were observed with microscope. TURNEL method and DNA fragmentation analysis were used for apoptosis confirmation. Survival cells were detected by AlamarBlueTM method.
视黄酸(retinoic acid,RA)诱导P19细胞分化,培养于明胶包被培养板上的P19细胞为对照组,普通光学显微镜观察其神经分化情况,TURNEL法和DNA片段化分析法检测细胞凋亡情况,alamarBlueTM染色法检测细胞存活情况。
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Assessment completed, the applicant had to borrow again to the evaluation report management center, a comprehensive assessment of the agency staff to further review the loan applications. For unexecuted formal purchase contract applicants, the loan agency personnel agreed that loans issued by credit pledge, loan applicants had to developers Department of Housing Land Authority executed Beijing uniform standard purchase contract printed text, persons must apply for the purchase of pre-sale auction registration.
申请人到其交存住房公积金的北京市住房资金管理中心或所属分中心提出贷款要求,向贷款经办人员如实介绍其所购房屋情况,本人及配偶的工作和收入情况,希望申请的金额和期限,其住房公积金的交存情况,可以提供什么样的担保,同时领取借款申请表及有关材料,由经办人员给申请人初步建议,告诉其需要准备的材料。
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ABSTRACT Power electronics devices are developed rapidly. There are some factors which influence the protective properties of the devices, including such as electrical characteristics of protection materials, the interface conditions of protective materials-pn junction, the surface effective charge density, the polarizable or unpolarizable properties of organic protective materials, etc.
电力半导体器件是近期发展起来的一类功率电子器件,其表面保护与其它电气元件不同,一方面要考虑器件表面绝缘保护材料的电性能和含杂等因素;另一方面,器件的表面钝化保护材料与器件接触后,器件的界面情况、材料与器件接触带电情况、以及材料所含官能团的极性等情况,也是影响器件性能的主要因素。
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Now consider a theory of perception according to which we have direct perceptual access to ordinary objects and to many ordinary properties (properties of external objects, not sense-data, etc.). In fact, as we saw above in the analogous case of "outside-a-room scepticism," we have to make this assumption if we are to have unproblematic access to things in the counter-domain. In such a case, and in contrast to the case of house arrest, the Humean argument does apply and does, apparently, go through.
现在考虑一个感知理论,据其我们直接感知性可达常识对象和可达许多常识属性(——关于外在对象的属性,而不是感觉材料的,等),事实上,如我们上面看到的,在屋外怀疑论的类似情况中,如果我们要不成问题地可达在反区域的事物,我们不得不做这个预设,而在这样的情况中,和屋子情况比较,Hume式论证显然被使用和执行。
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The fourth part, the students are carried on the questionnaire survey on zetetic experiment knowledge and the skill grasping situation, the formation situation, emotion manner and the values formation situation.
第四部分,对学生探究性实验知识与技能的掌握情况、学生实验探究能力的形成情况、学生对化学实验情感态度与价值观的形成情况进行问卷调查。
- 推荐网络例句
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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.
在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。
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In fact,I've got him on the satellite mobile right now.
实际上 我们已接通卫星可视电话了
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The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.
世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。