悬浮体

Embryogenic suspension cell is ideal material of protoplast culture of gramineous plants.

胚性悬浮细胞是禾谷类作物原生质体培养的理想材料。

Through designing of composition and structure of the bioactive graded coating,innerstress and its distribution in the coating were analyzed and calculated, the resultsshowed that when composition distribution coefficient n was 1.5, a reasonable stressdistribution could be got, that was at the beginning of deposition the suspension containingrichly BG granules was used so that a rich BG granules layer, a good transitional layerbetween BG layer at the bottom and the coating could be obtained at the titanium alloy side,the bottom of the coating; the stress value near the interface and surface and its character,pressure stress or tensile stress, were decided by the character of its composition itself.Changing composition distribution coefficient n could only change the variation tendency ofstress in the coating, but did not change the stress distribution rule in the coating. Thethinner the coating is, the sharper stress variation in the coating is, which does not mean thatthicker coating is better because the thicker the coating is, the little the permitteddeformation of coating is, so the coating thickness should be thinner, for example, about50μm for bending applications, but for applications only bearing pure shear stress, such asroot of tooth implant, the coating can be thicker little, for instance, about 80～100μm. The study on electrification characteristic and electrophoresis deposition of HAand BG granules in aqueous and non-aqueous solution system found that EPD almost didn'toccur in aqueous solution system. However, because HA granules take position charges inabsolute alcohol, a homogeneous EPD be carried out on the cathode titanium alloy slice, but taking negative charge in absolute alcohol the BG granules not be deposited on the cathode. A guided HA crystallizing, 100～300nm, on surface of the BG granules be realized by metathetical reaction, which cover BG granules with HA microcrystals and make the covered BG granules taking position charges in absolute alcohol, sequentially realize the EPCD of the BG and HA granules on the cathode, so it is feasible to make a titanium alloy/BG/HA bioactive graded coating by making use of EPCD technology. The corrosion experiment of rich boron bioglass coating and plasma spray coating showed that split phase, rich boron and rich silicon phase, occurred during its preparation. In basic medium the corrosion behavior of 〓 BG coating showed uniformity corrosion, the corrosion mostly occurred at rich boron phase area, therefore batch formula design of BGshould avoid the occurring of split phase. The corrosion appearance of plasma spray coatingappeared a non-uniform corrosion, mostly occurred at the edge of the laminated HA moltendrops, and emerged an accelerated corrosion tendency, which will easyly lead to corrosioncrackles extending to the interface and the happening of osmotic interfacial corrosion, thatmay be one of the major reasons leading to the coating cracking-off in the later period. Thetesting results of thermal expansion coefficient of 〓 and 〓BG showed the thermalexpansion coefficient of 〓 BG matched with that of titanium alloy better, and 〓 BG couldsinter with titanium alloy into densification enamel layer at low temperature (720℃).

将Ti6Al4V合金在1000℃下进行真空热处理会降低其力学性能，且合金内的V元素会向表面富集，因此，钛合金真空热处理和表面涂层的烧结温度不能过高，即应低于其相转变点；通过对生物活性梯度涂层的组成和结构的设计，分析和计算了梯度涂层内的应力大小和分布，结果表明：对于本研究，当成分分布系数n=1.5时，可以获得较合理的涂层力学性能，即在沉积开始时，采用富含BG颗粒的悬浮液，以便在钛合金侧获得同底层BG有良好过渡的富BG涂层；梯度涂层界面和表面的应力大小、性质由材料组成本身的性质决定，改变成分分布系数，只能改变涂层内应力变化的趋势；涂层的厚薄不影响涂层内的应力分布规律，但涂层越薄，涂层内的应力变化越快，但这并不意味着涂层越厚越好，因为涂层越厚，涂层允许的变形越小，对于应用于弯曲受力部位的涂层而言，涂层应薄一点为好(50μm)；而对于仅纯受剪切应力的部位，如牙根种植体，涂层可适当加厚(80～100μm)；通过对HA和BG颗粒在水溶液体系和非水溶液体系中的带电特性和电泳沉积的研究发现，它们在水溶液体系中很难发生电沉积；在无水乙醇溶液中，HA颗粒带正电，可在阴极钛合金片上发生均匀的电泳沉积，而BG颗粒则带负电荷；利用复分解反应法，可以制得100～300nm的HA，通过诱导HA在BG颗粒表面结晶，可对BG颗粒进行表面包覆，获得了被HA包覆的BG颗粒，改变了BG颗粒表面的带电特性，使BG和HA颗粒在无水乙醇中均带上正电荷，从而实现了HA和BG颗粒在阴极上的共沉积。

The highest yield of the two eucalypts from cell suspension cultures differed: 4.7×10^6/mL for E. macarthurii and 6.9×10^6/mL for E. radiata.

二树种由悬浮细胞分离原生质体的产量差异颇大，毛皮桉为4.7×10^5/mL，放射桉为6.9×10^6/mL。

Research results indicate that X FLOUR has notable effects of fluidizing and water reducing.

研究结果表明：X粉具有显著的流化与减水效应，其流化与减水效果与目前市售掺合料的最高水平相当，明显好于硅灰。X粉与硅灰复合后，仍具有流化效应。X粉产生流化效应的机理，一是增加水泥浆悬浮体系的分散性；二是通过填充效应减少填隙水，增加了用于混凝土流动性的水量；三是增加混凝土混合料的浆体体积。X粉流化与减水效应的发挥，以与高效减水剂双掺为前提。

This article studied improved the ZrO2 suspending liquid disperser effect the method, and ZrO2 compound has plated the system relative disperser fluid; After the optimization of the galvanized parameter, surface Galvanizes a ZrO2 coating on the surface of the alloy K17 with Ni on the base heat-resisting, and compares the coating degree of hardness value which the system results in under the different craft; Surveys and calculates its oxidized dynamics curve after passes through 1000℃ carries on 100h the high-temperature oxidation experiment, and analyzes it to the organizational structure through the metallography microscope, the substrate bound state and so on the influence.

本文研究了改善ZrO2悬浮液分散效果的方法，并制得了相对分散均匀的ZrO2复合镀液；通过优化电镀各项参数后，在Ni基高温合金K17表面上电镀一层ZrO2 镀层，并比较在不同工艺下制得的镀层的硬度值；经过在1000℃进行100h的高温氧化实验后，测量并计算其氧化动力学曲线，分析氧化对组织结构、基体结合等状态的影响。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

However, out of the 392 counted cells, no ploidy variated cell was found in the suspension used for the isolation of somatic protoplasts, all of them were diploid with 18 chromosomes.

而起始悬浮细胞系为二倍体，检查的392个细胞，未发现有染色体倍性变异。

The utilization and/or hydrolysis of sorbed or entrapped carbonaceous pollutants is a most important feature of these variants of the activated sludge process that have been designed specially for the treatment of wastewaters rich in colloidal and suspended pollutants.

&被吸附或被夹带的含碳污染物的利用与水解是活性污泥过程变体的一种重要的模式，这种模式是特别为处理富含胶体的悬浮的污染物的废水而设计的。&

Oil-less air compressors are mainly used in areas of medical treatment (oxygen-making equipments, oxygen-chambers, respirators, nebulizers, air-operated dental drill, cleaning equipments), cosmetology equipments, air-powered equipments (car doors, air cushion, air suspension, pressurization), sewage disposal, liquid filling machines, spinning and weaving, and printing.

猜猜系列无油空气压缩机广泛应用于医用制氧设备，医用氧舱，医用呼吸机，医用雾化器，气动牙钻，清洁护理机，美容美体机，汽车气动门，气垫、气悬浮，气动工具，污水处理，饮料灌装，气动加压，印刷机械，纺织机械等。

An idea that a station keeping diving can be used for a suitable reference benchmark had been put forward in the previous research on this topic-based group, and had carried on much research ahead of time, had obtained some results, but need further research.

本课题组提出了用悬浮潜体作参考基准的方法，并进行了先期研究。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失