总设计

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

Yet class after class,I watched boys dressed in blue blazers and gray pants head toward girls in flowered shifts whose perfect ponytails swung back and forth like metronomes.

一堂又一堂的舞蹈课，我看着穿蓝色运动茄克和灰色裤子的男孩总创意名片设计欣赏是把目标瞄向穿花衬衫的女孩子，这些女孩有节奏地前后甩动着漂亮的马尾辫。

The method was simple and quick, so it worthed popularizing and applying. Taking content of total hydroxybenzene as parameters, the extractive process was investigated by orthogonal design.

以总酚含量为考察指标，分别以水、35%、65%和95%乙醇作溶剂，利用正交设计法对苏木的渗漉法、索氏法、超声波法提取工艺进行了优化。

Total RNA was isolated using the method designed by ourselves. Full-length fragments of Apple sear skin viroid have been amplified by one specific pair of primer from different pear samples.

利用自行设计的方法分离提取总RNA，通过一对特异引物，分别扩增出不同梨树品种上苹果锈果类病毒的特异片段，通过对库尔勒香梨品种上获得的特异片段回收、克隆和测序。

Branches, leaves and phloem of apple collected from an old orchard in Yanji, Xinjiang were used for total RNA isolation specific fragments of Apple fruit crinkle viroid were amplified by RT-PCR using the primers disigned according to the sequence AB104558 on GenBank. Ten sequences were obtained through recovering, cloning and sequencing, and also registered in GenBank accession No.

采集新疆焉耆地区的老果园中的苹果树枝条、叶片和韧皮部，提取总RNA，根据GenBank中的AB104558序列设计引物，利用RT-PCR技术扩增出苹果皱果类病毒（Apple fruit crinkle viroid ，AFCVd）特异条带，通过回收、克隆和测序，结果表明，获得的10条序列，已在GenBank中登录（登录号：EU031507～EU031516）。

A varifocal f-theta lens was designed for Nd:YAG laser making scan focusing system, so that it apply to differ making area but change a lens necessarily. the system consists of four lens ,made by glass BK7, SF18 and SF11. The total length of the system is 122.96mm, working at 1064m.

为Nd：YAG激光打标机设计了变焦距f-theta镜扫描系统，以使它适用于不同的打标面积而不必更换镜头，系统由四片透镜组成，材料分别为BK7，SF18和SF11，系统总长度为122.96mm，工作波长1064nm。

Our products have been selected as source in OEM Form by many local and foreign famous mosquito killing incense plants ,aerosol companies and daily chemical companies,40% output for foreign market and 60% for domestic. At present our company is the leading manufacturer of electric vaporizers for mosquito killing in China with its quality up to international level.

几年来公司本着：&团结，求实，开拓，进取&的企业精神和追求专业打造&高品质，高品位&产品的质量理念，先后自行设计，开发出造型高雅，美观，实用的电热蚊香器等系列产品，品种达四十余种：以OEM形式为国内外各大蚊香，气雾剂，日化公司定牌、贴牌生产；出口占总销量的40％；目前电热蚊香器的产量已名列国内前矛，质量达到国际水平，产品深受国内外客商的青睐和好评。

In the society where trend is fast changing, fashion always exists by different ways. SHAENNA is the best present that DAHONGYING devoted to customers because of its trendiness and imagination.

在信息万变的时代，时尚总有它的不同存在方式，莎恩纳以其特有的潮流触角，充满想象力的设计风格，成为为顾客呈现的最好的礼物。

Each one an individually numbered watches are set with 698 Diamond, with a total weight of about 13.5 karats, a perfect performance watch the beautiful curves and angles, Piaget Polo Tourbillon Diamond is the perfect tabulation process and outstanding jewelry harmonious combination of artistic design.

每一个独立编号手表设置698钻石约13.5克拉，一个完美的表现看美丽的曲线和角度的总重量，伯爵波罗飞轮钻石是完美的制表工艺和杰出的珠宝设计艺术的和谐结合。

Filmmakers listed in any local film's roller credit in the following positions: Film Producer, Executive Producer, Production coordinator, Associate Producer, Director, Deputy Director, First Assistant Director, Line Producer, Scriptwriter, Actor/Actress, Dubbing Artist, Cinematographer, Assistant Cameraman, Lighting Designer, Film Editor, Assistant Film Editor, Art Director, Assistant Art Director, Costume Designer, Assistant Costume Designer, Action Choreographer, Assistant Stunt Coordinator, Effect Designer, Soundman, Post- Production recording and mixing technician in chief, Computer Effect Designer, Music Score Composer and Lyricist / Composer for movie theme song.

一 曾参与香港电影工作的电影从业员(名字出现於片头、片尾字幕之内)，职位包括：电影出品人、监制、统筹、策划、导演、执行导演、第一副导演、制片、编剧、演员、配音员、摄影师、助理摄影、灯光师、剪接师、副剪接师、美术指导及助理、服装造型设计师及助理、动作指导、副动作指导、音效设计师、收音师、后期制作混录总收音师、电脑效果设计，以及曾为电影配乐、作曲及填词的音乐工作者。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。