总合的

We have used GC to follow up the formation of epoxy acid and established the acidify condition; we have done series of parallel experiments to better the pivotal condensation reaction between acid and amine; In the cyclization step, we use cheap and available reagent-Lithium amide , rather than expensive LiHMDS, to proceed the reaction; and at last, we introduced green reagent-ozone to clear the phenyl group of nitrogen to avoid using heavy metal reagent-CAN. The total yield for this method is 29.8%.

通过GC监测，确定环氧酸的最佳酸化条件；对关键的酸和胺的缩合步骤，平行试验了多种条件；在环合一步，用价廉易得的氨基锂及催化量的HMDS代替昂贵的LiHMDS进行反应；氮上苯基的脱除采用臭氧，避免了重金属试剂-硝酸铈铵的使用，总收率为29.8％。

Since most of the large molecules containing phosphorus element have double bonds, it is necessary to consider the special structures of double bonds in order to deal with these large systems more accurately. So, based on density functional theory and electronegativity equalization principle, applying a general atom-bond electronegativity equalization method , using separated program, through regression and least-squares optimization , the parameters of atoms and bonds in molecules are determined . By these parameters , the charge distributions and the energy of some molecules containing phosphorus element, especial the fragment of DNA, RNA, are calculated . The results are quite good.

由于这些含磷生物大分子体系都含有双键，为了明确的处理双键，进而更准确的解释带有双键和共轭双键的含磷生物大分子结构与性质的关系，我们以密度泛函理论~([1-4])和电负性均衡原理~([5-9])为基础，应用和开发原子-键电负性均衡方法中的σπ模型，利用最小二乘法，拟合确定了一些分子（含碳、氮、氧、氢以及磷等原子）的各种类型原子及化学键区域的参数，并利用上述参数计算了一些多聚核苷酸的电荷分布以及分子的总能量，进行了讨论，得到的结果可以和从头算相媲美。

Problems of single footing are classifying about results、determination of dimension of combinational column on combinational foundation、determination of bending moment on superface of combinational foundation and determination of partly press area when there is angel between column and foundation; the problems of strip foundation are determination of torsional moment on some section in checking torsion resist.

独立基础中的问题主要是基础计算结果的归并、双柱合并基础合柱尺寸的确定、双柱合并基础顶面弯矩的求法及柱相对基础有转角时局部受压计算底面积的确定；柱下条形基础的问题是抗扭验算中总扭矩不为0情况下截面扭矩的确定。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Proximal margin of involucral bracts open or folded but not basally connate, base cordate or rounded.

开阔的的或折叠的总苞片但不是基部合生，基部心形或圆形的下部边缘。

The synthesis of 4,5,7-trichloroquinoline makes use of ethyl orthoformate, cyanoacetic ester and 3,5-dichloroaniline as the raw materials. The synthesis is completed by four steps: condensation, cyclization, hydrolyzation and chlorination. Several conditions concerned in the process are studied, the whole yield of 4,5,7-trichloroquinoline is about 53%, and the purity of it is about 96%. All the products are testified by IR, NMR and HPLC.

介绍了以3，5-二氯苯胺、原甲酸三乙酯和氰乙酸乙酯为原料，经过缩合、成环、水解和氯代4步反应制备4，5，7-三氯喹啉的方法，对相关的试验条件进行了考察，通过红外光谱、核磁共振谱和液相色谱对4，5，7-三氯喹啉的结构和纯度进行表征和测定，试验结果为4，5，7-三氯喹啉的纯度大于96%，总收率达到53%。

Therefore can pass to use the cupric sulfate sulfuric acid potassium for the catalyst take sulfuric acid as to carry the body to digest it, make the amino acids chelate match the conversion for dissolve sex easily of ammonium sulfate and white vitriol, then absorb a fixed amount to try the kind to join 40% sodium hydroside combine under the steam heat function make ammonia the spirit swims to leave out, through cool off to respond the born boric acid with boric acid, be got the total nitrogen content by the standard aqua titration of hydrochloric acid immediately after;But the white vitriol can immediately join the hydrochloric acid hydroxylate , fluoride to turn the in order to cover up, carry on the titration but get the content of its zinc with the EDTA mark liquid..

因此可以通过用硫酸铜硫酸钾为催化剂以硫酸为载体将其消化，使氨基酸螯合锌转化为易溶性的硫酸铵和硫酸锌，然后吸取一定量的试样加入40 %的氢氧化钠并在水蒸气高温作用下使氨气游离出来，经冷却与硼酸反应生成硼酸铵，接着由盐酸标准溶液滴定得到总氮含量；而硫酸锌即可加入盐酸羟铵、氟化氢铵为掩蔽剂，用EDTA标液进行滴定而得到其锌的含量。

The chemical analysis result of modified lignin by alkaline-oxygen treatment is as following:The chemical analysis result of modified lignin by alkaline-oxygen treatment showed that the carboxyl group of modified lignin increased, since muconic acid type structure were generated from phenolic structure by ring-open reaction during alkaline-oxygen treatment.

氧碱处理改性木素的化学分析结果见下表；┌────┬────┬─────┬────────────────┬────┐│改性本素│OCH_3│总糖│C_9单元式│COOH │├────┼────┼─────┼────────────────┼────┤│O_4 │8.49 │0.07 │C_9H_(10.45)O_(3.7)(OCH_3)_(053)│10.82 │└────┴────┴─────┴────────────────┴────┘氧碱处理改性木素的化学分析结果表明，改性木素的羧基含量明显增加，在氧碱处理的过程中，由于芳环的开裂，(来源：ABCe6论6d文网www.abclunwen.com)形成了己二烯二酸结构，因此改性木素具有较高的反应活性，易与Al~(3+)形成络合。

The results calculated by the Elovich rate equation showed that K release rates from 1 mol.L-1 HNO3 extraction ＞those from 2 mol.L-1 HNO3 extraction ＞those from H-resin extraction. Elovich equations combining with the data of H-resin and 2 mol.L-1 HNO3 successive extraction could well described the release characteristics of soil K. The correlation coefficients of the equations reached most significant levels (r＝0.956**—0.999**), and standard errors of specific K release capacities between the vaules calculated by the equations and those measured by both successive extractions were smaller(s=1.61 and 3.52). The specific release rates of soil K calculated by the Elovich rate equations were significantly correlated with the indices of soil K availability (the amounts of easily available K, nonexchangeable K, total available K in soils and K absorbed by ryegrass).

研究结果表明：1 mol.L-1 HNO3提取法的释K速率＞2 mol.L-1 HNO3提取法的释K速率＞氢饱和交换树脂提取法的释K速率；氢质交换树脂和2 mol.L-1 HNO3提取法结合Elovich模型较理想地描述了土壤K素的释放特性，拟合方程的相关系数均达极显著水平(r＝0.956**-0.999**)，土壤特征累积释K量的计算值与实测值的标准差较小(s=1.61和3.52)，且通过速率方程计算的土壤特征释K速率与K素有效性指标(速效K、缓效 K、有效K及黑麦草总吸K量)的相关性也较为密切。

Zhou Xunshu overcame a slow start — bogeys on two of his first three holes — to finish with a 1-under 71 in the opening round of the RMB 1 million (US$146,681) Dell Championship Thursday at Orient Golf Club in Xiamen, the first event on the 2009 Omega China Tour.

周训书在克服了开始时的一些不顺后—在前三洞中吞下两个柏忌—最后以71杆低于标准杆一杆的成绩结束了首轮的比赛，这场星期四开始在东方高尔夫俱乐部举办的戴尔锦标赛是2009欧米茄中巡赛的首场赛事，总奖金为人民币一佰万元(约合美金146，681)。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。