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All the recombined suicide plasmids were successfully obtained and sequenced, and 24 unique sequences were identified. Bioinformatics analysis showed that there were 51 ORFs in the 24 operons.

对插入的随机片段进行测序后进行生物信息学分析,共证实有24个不同的基因片段,在TIGR4全基因组里面,这24个基因片段所处的操纵元结构中,总共包括51个开放阅读框,其中多数为含有多个ORFs的操纵元结构。

There are six major types of openers.

总共有六大种的开启者。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy(32/120; 26.7%). The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders(22/120; 18.3%). Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found(12/120; 10%). Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters(8.3%). Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters(4.2%)had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现,在总共搜集的120个病例中,其中因年老所导致的产精力不佳为最多,占38例(31.7%),於镜下可见大量精虫黏附於Sertoli cell的表面,并可见Sertoli cell数量明显增多而Leydig cell明显减少,且其细胞核呈现浓染的现象,而在其副睪中仍可见到成熟精虫蓄留於管腔中,但在其输精管内却只有少量精虫存在;其次为睪丸萎缩,占32例(26.7%),镜下可见中度至重度无造精作用,其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在,但Leydig cell数量明显减少且其细胞质内的脂质也明显减少;睪丸发育不全,占22例(18.3%),於生精小管内只见到精母细胞及少量Sertoli cell存在,不见造精细胞分化,於生精小管间质可见Leydig cell减少并伴随结缔组织增生;副睪结石,占12例(10%),肉眼下可在副睪及输精管前段见到黄白色大小不一的结节,触感坚硬,於镜下可见副睪管扩张并有大量成熟精虫蓄积,严重时可见钙化现象,以Von Kossa及茜素红染色均呈阳性反应;类淀粉沉著症,占10例(8.3%),镜下在睪丸间质及血管周围可见粉红均质样的物质沉积,以刚果红染色成阳性反应;马立克病,占5例(4.2%),镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润;睪丸坏死,占1例(0.8%),镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

Gross 6 words, but environmental protection belt designs a contest this word appears in TITLE, appear in content, and related to its content is done not have, this also is Baidu considers as likely cogged.

总共6行字,但环保带设计大赛这个词在TITLE中出现,并在内容中出现一次,而与其相关内容没有,这也有可能是百度认为是作弊。

Altogether, ten million hectares of wheat and rape seed have been badly affected.

总共有一千万公顷的小麦和油菜种子受到严重影响。

Now, my sister is a master at saving; at our recent family reunion, she hosted two separate meals, a picnic and cookout, for more than 40 people for a total cost of less than $250, by shopping at Wal-Mart and using her well-practiced eye forbargains.

如今,我的妹妹擅长储蓄;在我们最近的家庭聚会中,她请40多人吃了两顿饭,凭借老道的购买低价商品的本事和在沃尔玛采购,总共只花了不到250美元。

Now, my sister is a master at saving; at our recent family reunion, she hosted two separate meals, a picnic and cookout, for more than 40 people for a total cost of less than $250, by shopping at Wal-Mart and using her well-practiced eye for bargains.

如今,我的妹妹擅长储蓄;在我们最近的家庭聚会中,她请40多人吃了两顿饭,凭借老道的购买低价商品的本事和在沃尔玛采购,总共只花了不到250美元。

Now, my sister is a master at saving ; at our recent family reunion, she host ed two separate meals, a picnic and cookout, for more than 40 people for a total cost of less than $250, by shopping at Wal-Mart and using her well-practiced eye for bargains.

如今,我的妹妹擅长储蓄;在我们最近的家庭聚会中,她请40多人吃了两顿饭,凭借老道的购买低价商品的本事和在沃尔玛采购,总共只花了不到250美元。

Now, my sister is a master at saving; at our recent family reunion,she hosted two separate meals, a picnic and cookout, for more than 40people for a total cost of less than $250, by shopping at Wal-Mart andusing her well-practiced eye for bargains.

如今,我的妹妹擅长储蓄;在我们最近的家庭聚会中,她请40多人吃了两顿饭,凭借老道的购买低价商品的本事和在沃尔玛采购,总共只花了不到250美元。

The course is made up of 12 two-hour sessions.

这门课总共上 12 次,每次两小时。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。